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Assessment of Genotoxic Effects of Pesticide Residues and Related Haemato-Biochemical Parameters on Farmed Nile Tilapia (Oreochromis Niloticus L.) in Kafrelsheikh Governorate, Egypt

Zizy I. Elbialy, Tamer Ismail, Doaa H. Abdelhady, Amel M. Elasely.

Abstract
Fish genotoxicity biomarkers are valuable parameters for environmental risk assessment. The aim of the current study was to determine the levels of organochlorine and organophosphorus pesticide residues in water and Nile tilapia fish samples collected from two fish farms in Kafrelsheikh governorate, Egypt. To detect the genotoxic effect on different fish tissues using comet assay and DNA fragmentation, as well as the effect on the haemato-biochemical parameters of the sampled fish. A large number of pesticide residues were detected both in the water and fish samples with high incidence of organochlorine compounds especially endrin, heptachlor epoxide, hexachlorobenzene (HCB), and p,p′-DDE. While the haematological parameters revealed a significant decrease in RBCs, PCV, HB, neutrophilia, lymphopenia and monocytosis. A Significant increase in the ALT, AST, urea and creatinine indicating hepato-toxicity and kidney damage. The phagocytic assay revealed a significant decrease in both phagocytic activity (PA) and phagocytic index (PI) (P<0.05). The comet assay showed higher tail moments in the gills. Our results indicated that continuous exposure of the farmed Nile tilapia to different pesticide residues resulted in significant haemato- biochemical alterations as well as significant damage of the DNA in vital organs like gills and liver.

Key words: Nile tilapia fish, Pesticide residues, haematological parameters, comet assay, DNA damage.


 
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How to Cite this Article
Pubmed Style

Elbialy ZI, Ismail T, Abdelhady DH, Elasely AM. Assessment of Genotoxic Effects of Pesticide Residues and Related Haemato-Biochemical Parameters on Farmed Nile Tilapia (Oreochromis Niloticus L.) in Kafrelsheikh Governorate, Egypt. AJVS. 2015; 44(1): 136-146. doi:10.5455/ajvs.176708


Web Style

Elbialy ZI, Ismail T, Abdelhady DH, Elasely AM. Assessment of Genotoxic Effects of Pesticide Residues and Related Haemato-Biochemical Parameters on Farmed Nile Tilapia (Oreochromis Niloticus L.) in Kafrelsheikh Governorate, Egypt. http://www.alexjvs.com/?mno=176708 [Access: August 21, 2018]. doi:10.5455/ajvs.176708


AMA (American Medical Association) Style

Elbialy ZI, Ismail T, Abdelhady DH, Elasely AM. Assessment of Genotoxic Effects of Pesticide Residues and Related Haemato-Biochemical Parameters on Farmed Nile Tilapia (Oreochromis Niloticus L.) in Kafrelsheikh Governorate, Egypt. AJVS. 2015; 44(1): 136-146. doi:10.5455/ajvs.176708



Vancouver/ICMJE Style

Elbialy ZI, Ismail T, Abdelhady DH, Elasely AM. Assessment of Genotoxic Effects of Pesticide Residues and Related Haemato-Biochemical Parameters on Farmed Nile Tilapia (Oreochromis Niloticus L.) in Kafrelsheikh Governorate, Egypt. AJVS. (2015), [cited August 21, 2018]; 44(1): 136-146. doi:10.5455/ajvs.176708



Harvard Style

Elbialy, Z. I., Ismail, T., Abdelhady, D. H. & Elasely, A. M. (2015) Assessment of Genotoxic Effects of Pesticide Residues and Related Haemato-Biochemical Parameters on Farmed Nile Tilapia (Oreochromis Niloticus L.) in Kafrelsheikh Governorate, Egypt. AJVS, 44 (1), 136-146. doi:10.5455/ajvs.176708



Turabian Style

Elbialy, Zizy I., Tamer Ismail, Doaa H. Abdelhady, and Amel M. Elasely. 2015. Assessment of Genotoxic Effects of Pesticide Residues and Related Haemato-Biochemical Parameters on Farmed Nile Tilapia (Oreochromis Niloticus L.) in Kafrelsheikh Governorate, Egypt. Alexandria Journal of Veterinary Sciences, 44 (1), 136-146. doi:10.5455/ajvs.176708



Chicago Style

Elbialy, Zizy I., Tamer Ismail, Doaa H. Abdelhady, and Amel M. Elasely. "Assessment of Genotoxic Effects of Pesticide Residues and Related Haemato-Biochemical Parameters on Farmed Nile Tilapia (Oreochromis Niloticus L.) in Kafrelsheikh Governorate, Egypt." Alexandria Journal of Veterinary Sciences 44 (2015), 136-146. doi:10.5455/ajvs.176708



MLA (The Modern Language Association) Style

Elbialy, Zizy I., Tamer Ismail, Doaa H. Abdelhady, and Amel M. Elasely. "Assessment of Genotoxic Effects of Pesticide Residues and Related Haemato-Biochemical Parameters on Farmed Nile Tilapia (Oreochromis Niloticus L.) in Kafrelsheikh Governorate, Egypt." Alexandria Journal of Veterinary Sciences 44.1 (2015), 136-146. Print. doi:10.5455/ajvs.176708



APA (American Psychological Association) Style

Elbialy, Z. I., Ismail, T., Abdelhady, D. H. & Elasely, A. M. (2015) Assessment of Genotoxic Effects of Pesticide Residues and Related Haemato-Biochemical Parameters on Farmed Nile Tilapia (Oreochromis Niloticus L.) in Kafrelsheikh Governorate, Egypt. Alexandria Journal of Veterinary Sciences, 44 (1), 136-146. doi:10.5455/ajvs.176708





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