E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Genotypic molecular detection of certain genes encoding virulence determinates and antibiotic resistance in Staphylococcus aureus isolates from mastitic cows

Ahmed Lotfy.

Abstract
A total of 10Staphylococcusaureusisolates were recoveredfrom 117 milk samples whichwere obtained from 93 dairy cows indifferent farms suffered fromclinical mastitis, theseisolateswere phenotypically characterized and identified based on colony morphology andbiochemical tests. Themolecular detection of S. aureus specific sequence gene(Sa),genes encoding clumpingfactor A(clfA), (spa) gene encoding S.aureus protein A and the class 1 integron using PolymeraseChain Reaction were carriedout. All isolateswere negative for the presence of class 1 integronbut yielded an amplicon size of about107 bp in case of S. aureus specific sequence gene (Sa),an amplicon size of about 638bp in case ofclfA gene. Amplification of thespa gene encoding S. aureus protein Awas detected in 9 strains out of 10 (90%)and yielded an amplicon size of about 226bp.

Key words: Molecular detection, Staphylococcus aureus isolates, mastitic cows


 
ARTICLE TOOLS
Abstract
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Ahmed Lotfy
on Google
on Google Scholar

How to Cite this Article
Pubmed Style

Ahmed Lotfy. Genotypic molecular detection of certain genes encoding virulence determinates and antibiotic resistance in Staphylococcus aureus isolates from mastitic cows. AJVS. 0; 0(0): -.


Web Style

Ahmed Lotfy. Genotypic molecular detection of certain genes encoding virulence determinates and antibiotic resistance in Staphylococcus aureus isolates from mastitic cows. https://www.alexjvs.com/?mno=216450 [Access: December 03, 2023].


AMA (American Medical Association) Style

Ahmed Lotfy. Genotypic molecular detection of certain genes encoding virulence determinates and antibiotic resistance in Staphylococcus aureus isolates from mastitic cows. AJVS. 0; 0(0): -.



Vancouver/ICMJE Style

Ahmed Lotfy. Genotypic molecular detection of certain genes encoding virulence determinates and antibiotic resistance in Staphylococcus aureus isolates from mastitic cows. AJVS. (0), [cited December 03, 2023]; 0(0): -.



Harvard Style

Ahmed Lotfy (0) Genotypic molecular detection of certain genes encoding virulence determinates and antibiotic resistance in Staphylococcus aureus isolates from mastitic cows. AJVS, 0 (0), -.



Turabian Style

Ahmed Lotfy. 0. Genotypic molecular detection of certain genes encoding virulence determinates and antibiotic resistance in Staphylococcus aureus isolates from mastitic cows. Alexandria Journal of Veterinary Sciences, 0 (0), -.



Chicago Style

Ahmed Lotfy. "Genotypic molecular detection of certain genes encoding virulence determinates and antibiotic resistance in Staphylococcus aureus isolates from mastitic cows." Alexandria Journal of Veterinary Sciences 0 (0), -.



MLA (The Modern Language Association) Style

Ahmed Lotfy. "Genotypic molecular detection of certain genes encoding virulence determinates and antibiotic resistance in Staphylococcus aureus isolates from mastitic cows." Alexandria Journal of Veterinary Sciences 0.0 (0), -. Print.



APA (American Psychological Association) Style

Ahmed Lotfy (0) Genotypic molecular detection of certain genes encoding virulence determinates and antibiotic resistance in Staphylococcus aureus isolates from mastitic cows. Alexandria Journal of Veterinary Sciences, 0 (0), -.





Most Viewed Articles
Most Accessed Articles

  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Most Downloaded
    Top Downloaded Articles

  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Effect of Excess Fluoride on Reproductive Potentials in Farm Animals (Ovine)
    Ghada H. Abdel-Rahman, Hanaa A. El-Hallawany, Dohreig RA.
    AJVS. 2018; 57(2): 41-57
    » Abstract » doi: 10.5455/ajvs.296364

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract » doi: 10.5455/ajvs.47290

  • Most Cited Articles
    Most Cited Articles