E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Research (Original Article) 


Molecular Characterization of Diarrheagenic E. Coli Isolated From Newly Born Calves and Humans

Eman Khalifa, Mohammad A. Nossair, Mousa A. Ayoub, Ghada A. Hadad.

Abstract
The current study was conducted to study the molecular characterization of diarrheagenic Escherichia coli in neonatal calves and human. A total of 100 faecal samples were collected from diarrheic calves up to 3-months old. Also, 100 stool specimens were gathered from diarrheic humans during the period between April and July, 2016. Samples were screened for isolation of E. coli using standard laboratory procedures. 17 isolates of E. coli were recovered from calves (9) and human (8). Serological identification of E. coli recovered from calves revealed the presence of O26 (2), O118 (1), O111 (2), O157:H7 (3) and O103 (1) while that of E. coli isolates recovered from humans clarified the presence of O91:H21 (2), O121:H7 (1), O114:H2 (3) and O44:H18 (2). The antimicrobial resistance of the identified serogroups clarified that E. coli O26 serotype was sensitive to Enrofloxacin, Spectinomycin and Neomycin, E. coli O118 serotype was sensitive to Tetracycline, Spectinomycin and Neomycin, E. coli O111 serotype was moderately susceptible to Enrofloxacin, Amoxicillin, Spectinomycin and Neomycin, E. coli O 157 serotype was sensitive to Amoxicillin while moderately susceptible to Cefotaxime and Tetracycline and finally E. coli O 103 serotype was sensitive to Enrofloxacin only. On the other side, results of antimicrobial resistance of Enteropathogenic E. coli serogroups recovered from diarrheic humans revealed that E. coli O91:H21 was sensitive to Amoxicillin and Erythromycin and moderately sensitive to Ciprofloxacin, Enrofloxacin and Oxytetracycline while it was resistant to Flumequine and Doxycycline. Also, E. coli O121:H7 was sensitive to Ciprofloxacin and Amoxicillin while it was resistant to Flumequine, Enrofloxacin and Doxycycline. In addition, E. coli O114:H2 was resistant to Amoxicillin only. Lastly, E. coli O44:H18 was found to sensitive and moderately sensitive to the used antibiotic. Finally, molecular characterization of the obtained isolated using multiplex PCR for stx1, stx2 and eaeA genes for characterization of E. coli was performed. The obtained results indicated that the application of multiplex PCR assay was important to develop a highly sensitive and specific diagnostic method for rapid detection of E. coli in diarrheic calves and humans.

Key words: Diarrheic calves, human, E. coli, Isolation, Multiplex PCR


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Eman Khalifa
Articles by Mohammad A. Nossair
Articles by Mousa A. Ayoub
Articles by Ghada A. Hadad
on Google
on Google Scholar
Article Statistics
 Viewed: 1713
Downloaded: 259
Cited: 0


How to Cite this Article
Pubmed Style

Khalifa E, Nossair MA, Ayoub MA, Hadad GA. Molecular Characterization of Diarrheagenic E. Coli Isolated From Newly Born Calves and Humans. AJVS. 2019; 60(1): 55-62. doi:10.5455/ajvs.22828


Web Style

Khalifa E, Nossair MA, Ayoub MA, Hadad GA. Molecular Characterization of Diarrheagenic E. Coli Isolated From Newly Born Calves and Humans. http://www.alexjvs.com/?mno=22828 [Access: July 22, 2019]. doi:10.5455/ajvs.22828


AMA (American Medical Association) Style

Khalifa E, Nossair MA, Ayoub MA, Hadad GA. Molecular Characterization of Diarrheagenic E. Coli Isolated From Newly Born Calves and Humans. AJVS. 2019; 60(1): 55-62. doi:10.5455/ajvs.22828



Vancouver/ICMJE Style

Khalifa E, Nossair MA, Ayoub MA, Hadad GA. Molecular Characterization of Diarrheagenic E. Coli Isolated From Newly Born Calves and Humans. AJVS. (2019), [cited July 22, 2019]; 60(1): 55-62. doi:10.5455/ajvs.22828



Harvard Style

Khalifa, E., Nossair, . M. A., Ayoub, . M. A. & Hadad, . G. A. (2019) Molecular Characterization of Diarrheagenic E. Coli Isolated From Newly Born Calves and Humans. AJVS, 60 (1), 55-62. doi:10.5455/ajvs.22828



Turabian Style

Khalifa, Eman, Mohammad A. Nossair, Mousa A. Ayoub, and Ghada A. Hadad. 2019. Molecular Characterization of Diarrheagenic E. Coli Isolated From Newly Born Calves and Humans. Alexandria Journal of Veterinary Sciences, 60 (1), 55-62. doi:10.5455/ajvs.22828



Chicago Style

Khalifa, Eman, Mohammad A. Nossair, Mousa A. Ayoub, and Ghada A. Hadad. "Molecular Characterization of Diarrheagenic E. Coli Isolated From Newly Born Calves and Humans." Alexandria Journal of Veterinary Sciences 60 (2019), 55-62. doi:10.5455/ajvs.22828



MLA (The Modern Language Association) Style

Khalifa, Eman, Mohammad A. Nossair, Mousa A. Ayoub, and Ghada A. Hadad. "Molecular Characterization of Diarrheagenic E. Coli Isolated From Newly Born Calves and Humans." Alexandria Journal of Veterinary Sciences 60.1 (2019), 55-62. Print. doi:10.5455/ajvs.22828



APA (American Psychological Association) Style

Khalifa, E., Nossair, . M. A., Ayoub, . M. A. & Hadad, . G. A. (2019) Molecular Characterization of Diarrheagenic E. Coli Isolated From Newly Born Calves and Humans. Alexandria Journal of Veterinary Sciences, 60 (1), 55-62. doi:10.5455/ajvs.22828





Most Viewed Articles
  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract & References

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract & References » doi: 10.5455/ajvs.45443

  • Evaluation of a Rapid Immunochromatographic Test for Detection of Brucella Abortus Antibodies in Egyptian Cattle Sera and Milk
    Tharwat M. Elshemey, Amir H. Abd-Elrahman
    AJVS. 2014; 40(1): 24-28
    » Abstract & References » doi: 10.5455/ajvs.47307

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Surgical and Histological Evaluation of the Effectiveness of Propolis on Wound Healing
    Ramadan El Sayed Abd Elwahed, Mahmoud Hussein El Kammar, Islam Reda Edrees
    AJVS. 2013; 39(1): 52-63
    » Abstract & References

  • Effect of Lead Toxicity on Mineral Metabolism and Immunological Factors in Rats
    Nabil M Taha, Mahdy S. Korshom, abd El-Wahab M Mandour, Mohamed A Lebdah, Eman S aladham
    AJVS. 2013; 39(1): 64-73
    » Abstract & References

  • Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate
    Sarah E. AlBeltagi, Helmy A. Torky, Mahmoud E. Seddeek
    AJVS. 2014; 40(1): 44-51
    » Abstract & References » doi: 10.5455/ajvs.46652

  • Improving Production Traits for El-salam and Mandarah Chicken Strains by Crossing I- Estimation of Crossbreeding Effects for Growth Production Traits
    Ayman El-Sayed Taha, Fawzy Ali Abd El-Ghany
    AJVS. 2013; 39(1): 18-30
    » Abstract & References

  • Most Downloaded
  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Effect of Sex, Age and Time of the Day on Vital Parameters of Apparently Healthy West African Dwarf Goats in Abeokuta, Nigeria
    Bello, S.A., Akintunde, O. G., Sonibare, A. O., Otesile, E. B.
    AJVS. 2016; 49(2): 18-23
    » Abstract & References » doi: 10.5455/ajvs.209718

  • Growth Performance, Immune Response, Blood serum parameters, Nutrient Digestibility and Carcass Traits of Broiler Chicken as Affected by Dietary Supplementation of Garlic Extract (Allicin)
    Mohamed I. El-katcha, Mosad.A. Soltan, Mohamed M. Sharaf and Adel Hasen
    AJVS. 2016; 49(2): 50-64
    » Abstract & References » doi: 10.5455/ajvs.219261

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Detection of Some Pathogenic Organisms From Dairy Farm Milk
    Ahlam A. El-Leboudy, Amr A. Amer and Sarah Abd El Mohsen
    AJVS. 2015; 44(1): 111-118
    » Abstract & References » doi: 10.5455/ajvs.47762

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract & References » doi: 10.5455/ajvs.47290

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract & References » doi: 10.5455/ajvs.45443

  • Evaluation of a Rapid Immunochromatographic Test for Detection of Brucella Abortus Antibodies in Egyptian Cattle Sera and Milk
    Tharwat M. Elshemey, Amir H. Abd-Elrahman
    AJVS. 2014; 40(1): 24-28
    » Abstract & References » doi: 10.5455/ajvs.47307

  • Most Cited Articles
  • Antifibrotic Effect of Curcumin on Thioacetamide Induced Liver Fibrosis
    Suzan E. Abdou, Nabil M. Taha, Abd El-Wahab A. Mandour, Mohamed A. Lebda, Hanaa R. El Hofi, Amany MSE El-Morshedy
    AJVS. 2015; 45(1): 43-50
    » Abstract & References » doi: 10.5455/ajvs.179458
    Cited : 4 times [Click to see citing articles]

  • Adverse Effects of Ivermectin in comparison with Rafoxanide on Male Rats
    Abd El-Salam F. El-Sawy, Zeynab Kh. El-Maddawy and Soaad A. Seed
    AJVS. 2015; 47(1): 119-128
    » Abstract & References » doi: 10.5455/ajvs.198997
    Cited : 4 times [Click to see citing articles]

  • Enumeration and Characterization of Aeromonas spp. Isolated from Milk and Some Dairy Products in Sharkia Governorate, Egypt
    Nagwa I. ElBalat, Salah F. Abd-ElAal, Madeha A. Ayoub, Magdy S. Elsayed
    AJVS. 2014; 40(1): 52-64
    » Abstract & References » doi: 10.5455/ajvs.49073
    Cited : 3 times [Click to see citing articles]

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333
    Cited : 3 times [Click to see citing articles]

  • Detection of Molecular Characterization of Salmonella enterica isolated from Chicken Meat and Its Products by Using Multiplex PCR
    Heba S. Amin and Abd El-Rahman A. Abd El-Rahman
    AJVS. 2015; 46(1): 155-160
    » Abstract & References » doi: 10.5455/ajvs.196302
    Cited : 3 times [Click to see citing articles]

  • The Growth Promoting Effect of Beta-glucan in Comparison with Sodium Butyrate in Broiler Chicks
    Abd El-Salam F. El-Sawy, Zeynab Kh. El-Maddawy, Hawary S. Ibrahiem and El-Sadeq Bo-Ghazel
    AJVS. 2015; 44(1): 23-37
    » Abstract & References » doi: 10.5455/ajvs.163992
    Cited : 3 times [Click to see citing articles]

  • The Effect of Different Intervention Strategies to Alleviate Heat Stress on Behavior, Performance and Some Blood Parameters of Growing Muscovy Ducks
    Hebatallah E. El-Shafaei, Mohamed M. Sharaf, Rashed R. Rashed
    AJVS. 2016; 48(2): 69-76
    » Abstract & References » doi: 10.5455/ajvs.209177
    Cited : 3 times [Click to see citing articles]

  • Effectiveness of prebiotic as an alternative to the antimicrobial growth promoter on growth performance, blood constituents, intestinal healthiness and immunity of broilers.
    Mohamed S. Helal, Fatma M. Youssef, Mohamed K. Moursi, Waleed F. Khalil, Mohamed M. Abdel-Daim
    AJVS. 2015; 45(1): 13-25
    » Abstract & References » doi: 10.5455/ajvs.179869
    Cited : 3 times [Click to see citing articles]

  • Garlic Powder Attenuates Apoptosis Associated with Lead Acetate-Induced Testicular Damage in Adult Male Rats
    Nasr E. Nasr, Mostafa A. Elmadawy, Essam A. Almadaly, Walied Abdo, Mona M. Zamel
    AJVS. 2017; 54(1): 70-78
    » Abstract » doi: 10.5455/ajvs.268149
    Cited : 3 times [Click to see citing articles]

  • Evaluation of a Rapid Immunochromatographic Test for Detection of Brucella Abortus Antibodies in Egyptian Cattle Sera and Milk
    Tharwat M. Elshemey, Amir H. Abd-Elrahman
    AJVS. 2014; 40(1): 24-28
    » Abstract & References » doi: 10.5455/ajvs.47307
    Cited : 2 times [Click to see citing articles]