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Original Article 


Effect of different extenders on freezability and mitochondrial activity of Arab stallion spermatozoa

Aly A. El-Sharkawy, Sayed A Hattab, Ismail I. Abo-Ghaniema, El-Garhy N El-Garhy, Magdy kh Soliman.

Abstract
This study aimed to compare the efficiency of four cryodiluents on freezability and mitochondrial activity of Arab stallion spermatozoa. Semen samples were collected from 10 Arab stallions, diluted with modified INRA-82 (m-INRA), modified Kenney’s (m-Kenney) and or Eqi-Pro® diluents and subjected to cryopreservation. Post-thaw motility, membrane, acrosomal and mitochondrial integrities were evaluated. The results revealed the superiority of m-INRA over m-Kenney and or Eqi-Pro® in terms of post-thaw motility (52.50 ± 2.27 vs. 43.00 ± 2.13 and 41.50 ± 2.59%, respectively), viability index (134.25 ± 5.99 vs. 97.50 ± 6.40 and 94.75 ± 7.33, respectively), sperm membrane (46.60 ± 2.05 vs. 36.70 ± 1.73 and 36.60 ± 2.09%, respectively) and acrosome integrities (54.50 ± 1.30 vs. 43.00 ± 1.86 and 43.5 ±1.86%). Activities of stallion sperm mitochondria were better preserved using m-INRA as cryodiluent. Using Kenney’s extender resulted in the lowest sperm motility and integrities of acrosome, membranes and mitochondria. In conclusion, the motility as well as functional integrities of Arab stallion spermatozoa could be enhanced using m-INRA as a cryodiluent.

Key words: Extenders, mitochondrial activity, Arab stallion, spermatozoa


 
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Pubmed Style

Aly A. El-Sharkawy, Sayed A Hattab, Ismail I. Abo-Ghaniema, El-Garhy N El-Garhy, Magdy kh Soliman. Effect of different extenders on freezability and mitochondrial activity of Arab stallion spermatozoa. AJVS. 0; 0(0): -.

Web Style

Aly A. El-Sharkawy, Sayed A Hattab, Ismail I. Abo-Ghaniema, El-Garhy N El-Garhy, Magdy kh Soliman. Effect of different extenders on freezability and mitochondrial activity of Arab stallion spermatozoa. https://www.alexjvs.com/?mno=240986 [Access: December 03, 2023].

AMA (American Medical Association) Style

Aly A. El-Sharkawy, Sayed A Hattab, Ismail I. Abo-Ghaniema, El-Garhy N El-Garhy, Magdy kh Soliman. Effect of different extenders on freezability and mitochondrial activity of Arab stallion spermatozoa. AJVS. 0; 0(0): -.


Vancouver/ICMJE Style

Aly A. El-Sharkawy, Sayed A Hattab, Ismail I. Abo-Ghaniema, El-Garhy N El-Garhy, Magdy kh Soliman. Effect of different extenders on freezability and mitochondrial activity of Arab stallion spermatozoa. AJVS. (0), [cited December 03, 2023]; 0(0): -.


Harvard Style

Aly A. El-Sharkawy, Sayed A Hattab, Ismail I. Abo-Ghaniema, El-Garhy N El-Garhy, Magdy kh Soliman (0) Effect of different extenders on freezability and mitochondrial activity of Arab stallion spermatozoa. AJVS, 0 (0), -.


Turabian Style

Aly A. El-Sharkawy, Sayed A Hattab, Ismail I. Abo-Ghaniema, El-Garhy N El-Garhy, Magdy kh Soliman. 0. Effect of different extenders on freezability and mitochondrial activity of Arab stallion spermatozoa. Alexandria Journal of Veterinary Sciences, 0 (0), -.


Chicago Style

Aly A. El-Sharkawy, Sayed A Hattab, Ismail I. Abo-Ghaniema, El-Garhy N El-Garhy, Magdy kh Soliman. "Effect of different extenders on freezability and mitochondrial activity of Arab stallion spermatozoa." Alexandria Journal of Veterinary Sciences 0 (0), -.


MLA (The Modern Language Association) Style

Aly A. El-Sharkawy, Sayed A Hattab, Ismail I. Abo-Ghaniema, El-Garhy N El-Garhy, Magdy kh Soliman. "Effect of different extenders on freezability and mitochondrial activity of Arab stallion spermatozoa." Alexandria Journal of Veterinary Sciences 0.0 (0), -. Print.


APA (American Psychological Association) Style

Aly A. El-Sharkawy, Sayed A Hattab, Ismail I. Abo-Ghaniema, El-Garhy N El-Garhy, Magdy kh Soliman (0) Effect of different extenders on freezability and mitochondrial activity of Arab stallion spermatozoa. Alexandria Journal of Veterinary Sciences, 0 (0), -.




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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