E-ISSN 2536-9520 | ISSN 1110-2047
 
2024, Vol: 80, Issue: 1
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Original Article 


Detection the Difference in Growth of Equine Herpesvirus-1 and 4 in Vitro By Using Microscopic Examinations and Flowcytometric Analysis

Hassan Y.A.H. Mahmoud.

Abstract
Equine herpesvirus - 1 (EHV-1) and Equine herpesvirus - 4 (EHV-4) are endemic in horse over the entire world, in the study we compared between the replication of EHV-1 and 4 in an equine-derived cell line (FHK-Tcl3.1) by using microscopic examination and flowcytometric analysis. The result of EHV-1 showed that the CPE effect in the FHK-Tcl3.1 cells appeared after 24 hr from infection, but in case of EHV-4 cytopathic effect (CPE) appeared after 48 hr from infection. The flowcytometric analysis result for detection the percent of infected cells by both viruses, were (19.7 %) after four days from infection in EHV-4, compared to EHV-1 (50.3%), which the cells harvest in the second day from infection. These data suggested that the difference in cellular entry might be playing the decisive role in determining pathogenicity of EHV- 1 and EHV-4.

Key words: EHV-1 and EHV-4


 
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Pubmed Style

Hassan Y.A.H. Mahmoud. Detection the Difference in Growth of Equine Herpesvirus-1 and 4 in Vitro By Using Microscopic Examinations and Flowcytometric Analysis. AJVS. 2017; 52(1): 97-102. doi:10.5455/ajvs.254249

Web Style

Hassan Y.A.H. Mahmoud. Detection the Difference in Growth of Equine Herpesvirus-1 and 4 in Vitro By Using Microscopic Examinations and Flowcytometric Analysis. https://www.alexjvs.com/?mno=254249 [Access: December 07, 2023]. doi:10.5455/ajvs.254249

AMA (American Medical Association) Style

Hassan Y.A.H. Mahmoud. Detection the Difference in Growth of Equine Herpesvirus-1 and 4 in Vitro By Using Microscopic Examinations and Flowcytometric Analysis. AJVS. 2017; 52(1): 97-102. doi:10.5455/ajvs.254249


Vancouver/ICMJE Style

Hassan Y.A.H. Mahmoud. Detection the Difference in Growth of Equine Herpesvirus-1 and 4 in Vitro By Using Microscopic Examinations and Flowcytometric Analysis. AJVS. (2017), [cited December 07, 2023]; 52(1): 97-102. doi:10.5455/ajvs.254249


Harvard Style

Hassan Y.A.H. Mahmoud (2017) Detection the Difference in Growth of Equine Herpesvirus-1 and 4 in Vitro By Using Microscopic Examinations and Flowcytometric Analysis. AJVS, 52 (1), 97-102. doi:10.5455/ajvs.254249


Turabian Style

Hassan Y.A.H. Mahmoud. 2017. Detection the Difference in Growth of Equine Herpesvirus-1 and 4 in Vitro By Using Microscopic Examinations and Flowcytometric Analysis. Alexandria Journal of Veterinary Sciences, 52 (1), 97-102. doi:10.5455/ajvs.254249


Chicago Style

Hassan Y.A.H. Mahmoud. "Detection the Difference in Growth of Equine Herpesvirus-1 and 4 in Vitro By Using Microscopic Examinations and Flowcytometric Analysis." Alexandria Journal of Veterinary Sciences 52 (2017), 97-102. doi:10.5455/ajvs.254249


MLA (The Modern Language Association) Style

Hassan Y.A.H. Mahmoud. "Detection the Difference in Growth of Equine Herpesvirus-1 and 4 in Vitro By Using Microscopic Examinations and Flowcytometric Analysis." Alexandria Journal of Veterinary Sciences 52.1 (2017), 97-102. Print. doi:10.5455/ajvs.254249


APA (American Psychological Association) Style

Hassan Y.A.H. Mahmoud (2017) Detection the Difference in Growth of Equine Herpesvirus-1 and 4 in Vitro By Using Microscopic Examinations and Flowcytometric Analysis. Alexandria Journal of Veterinary Sciences, 52 (1), 97-102. doi:10.5455/ajvs.254249




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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