E-ISSN 2536-9520 | ISSN 1110-2047
 
2024, Vol: 80, Issue: 1
80 / 1Current Issue Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Original Article 


Development and Validation of Protein G Based Indirect ELISA Versus Lateral Flow Assay As Screening Immunoassays for Brucellosis in Camels (Camelus Dromedarius)

Nour H. Abdel-Hamid, Rania I. Ismail, Eman I.M. Beleta, Hazem M. Ghobashy.

Abstract
Two hundred selected positive and negative serum samples were collected from dromedary she-camels aged from 1.5-3 years during the period of January 2015 to February 2016 from the market and abattoirs in Halayeb-Shalateen and Abu-Simbel districts and with a history of Brucella melitensis biovar 3 isolation. This was aimed for validation of lateral flow assay (LFA) and in-house indirect enzyme-linked immunosorbent assay (iELISA) to be used in the diagnosis of camel brucellosis. The highest relative sensitivity (98%) was achieved by in-house iELISA coated with lipopolysaccharide antigen and with protein G conjugate -for the first time ever- in the diagnosis of camel brucellosis. LFA offered better performance in terms of relative sensitivity (92%), relative specificity (92.5%) and performance index (184.5). The overall performance of the LFA and in-house iELISA in camels based on both receiver operating characteristic curves (ROCs) and area under ROCs (AUCs) was very good being equal or closer to 0.9. LFA revealed better accuracy than other screening immunoassays tried. The highest positive predictive value was achieved by LFA (0.95), while the corresponding highest negative predictive value (NPV) was attained by in-house iELISA (0.97). All the screening serological tests agreed significantly with LFA and in-house iELISA (LPS). Based on the large association between both LFA, in-house iELISA and the other immunoassays, along with their better diagnostic performance characteristics, authors concluded that both tests were fit for their purpose and valid to be used as rapid screening tests for camel brucellosis as well as useful additions tools to the control and eradication programs in such animal species.

Key words: brucellosis; LFA; iELISA; camel; ROCs; protein G


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Nour H. Abdel-Hamid
Articles by Rania I. Ismail
Articles by Eman I.M. Beleta
Articles by Hazem M. Ghobashy
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Nour H. Abdel-Hamid, Rania I. Ismail, Eman I.M. Beleta, Hazem M. Ghobashy. Development and Validation of Protein G Based Indirect ELISA Versus Lateral Flow Assay As Screening Immunoassays for Brucellosis in Camels (Camelus Dromedarius). AJVS. 2017; 53(1): 11-20. doi:10.5455/ajvs.256953

Web Style

Nour H. Abdel-Hamid, Rania I. Ismail, Eman I.M. Beleta, Hazem M. Ghobashy. Development and Validation of Protein G Based Indirect ELISA Versus Lateral Flow Assay As Screening Immunoassays for Brucellosis in Camels (Camelus Dromedarius). https://www.alexjvs.com/?mno=256953 [Access: December 03, 2023]. doi:10.5455/ajvs.256953

AMA (American Medical Association) Style

Nour H. Abdel-Hamid, Rania I. Ismail, Eman I.M. Beleta, Hazem M. Ghobashy. Development and Validation of Protein G Based Indirect ELISA Versus Lateral Flow Assay As Screening Immunoassays for Brucellosis in Camels (Camelus Dromedarius). AJVS. 2017; 53(1): 11-20. doi:10.5455/ajvs.256953


Vancouver/ICMJE Style

Nour H. Abdel-Hamid, Rania I. Ismail, Eman I.M. Beleta, Hazem M. Ghobashy. Development and Validation of Protein G Based Indirect ELISA Versus Lateral Flow Assay As Screening Immunoassays for Brucellosis in Camels (Camelus Dromedarius). AJVS. (2017), [cited December 03, 2023]; 53(1): 11-20. doi:10.5455/ajvs.256953


Harvard Style

Nour H. Abdel-Hamid, Rania I. Ismail, Eman I.M. Beleta, Hazem M. Ghobashy (2017) Development and Validation of Protein G Based Indirect ELISA Versus Lateral Flow Assay As Screening Immunoassays for Brucellosis in Camels (Camelus Dromedarius). AJVS, 53 (1), 11-20. doi:10.5455/ajvs.256953


Turabian Style

Nour H. Abdel-Hamid, Rania I. Ismail, Eman I.M. Beleta, Hazem M. Ghobashy. 2017. Development and Validation of Protein G Based Indirect ELISA Versus Lateral Flow Assay As Screening Immunoassays for Brucellosis in Camels (Camelus Dromedarius). Alexandria Journal of Veterinary Sciences, 53 (1), 11-20. doi:10.5455/ajvs.256953


Chicago Style

Nour H. Abdel-Hamid, Rania I. Ismail, Eman I.M. Beleta, Hazem M. Ghobashy. "Development and Validation of Protein G Based Indirect ELISA Versus Lateral Flow Assay As Screening Immunoassays for Brucellosis in Camels (Camelus Dromedarius)." Alexandria Journal of Veterinary Sciences 53 (2017), 11-20. doi:10.5455/ajvs.256953


MLA (The Modern Language Association) Style

Nour H. Abdel-Hamid, Rania I. Ismail, Eman I.M. Beleta, Hazem M. Ghobashy. "Development and Validation of Protein G Based Indirect ELISA Versus Lateral Flow Assay As Screening Immunoassays for Brucellosis in Camels (Camelus Dromedarius)." Alexandria Journal of Veterinary Sciences 53.1 (2017), 11-20. Print. doi:10.5455/ajvs.256953


APA (American Psychological Association) Style

Nour H. Abdel-Hamid, Rania I. Ismail, Eman I.M. Beleta, Hazem M. Ghobashy (2017) Development and Validation of Protein G Based Indirect ELISA Versus Lateral Flow Assay As Screening Immunoassays for Brucellosis in Camels (Camelus Dromedarius). Alexandria Journal of Veterinary Sciences, 53 (1), 11-20. doi:10.5455/ajvs.256953




Most Viewed Articles
Most Accessed Articles

  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

    •
    Most Downloaded
    Top Downloaded Articles

  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Effect of Excess Fluoride on Reproductive Potentials in Farm Animals (Ovine)
    Ghada H. Abdel-Rahman, Hanaa A. El-Hallawany, Dohreig RA.
    AJVS. 2018; 57(2): 41-57
    » Abstract » doi: 10.5455/ajvs.296364

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract » doi: 10.5455/ajvs.47290

    •
    Most Cited Articles
    Most Cited Articles

    About Alexandria Journal of Veterinary Sciences

    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



    For best results, please use Internet Explorer or Google Chrome.

    Contact Information

    Faculty of Veterinary Medicine, Alexandria University
    Edfina, Behaira, Egypt.
    PO.Box: 22758
    Telefax: +20 45 2963526
    POLICIES & JOURNAL LINKS
    Author Login
    Reviewer Login
    About Publisher
    Advertising Policy
    Author's Rights and Obligations
    Conflict of Interest Policy
    Copyright Information
    Digital Archiving & Preservation Policies
    Editorial Policies
    Peer Review Policy
    Editorial & Peer Review Process
    License Information
    Open Access Policy
    Plagiarism Policy
    Privacy Policy
    Protection of Research Participants (Statement On Human And Animal Rights)
    Publication Ethics and Publication Malpractice Statement
    Corrections, Retractions & Expressions of Concern
    Self-Archiving Policies
    Statement of Informed Consent
    Terms of Use