E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Research (Original Article) 


Phylogenetic Characterization of Infectious Bursal Disease (IBD) Viruses Isolated From Field Outbreaks in Chickens From Behera And Alexandria Governorates, Egypt

Nahed A. El-shall, Mahmoud E. Sedeek, Muhammed M. El-badawy, Eid G. Hussein, Ashraf M. Awad.

Abstract
Infectious bursal disease continue to be happened in Egypt causing a great economic losses in
chickens. Thirty chicken flocks showing clinical signs suspected to be due to IBDV infection
have been investigated from Behera and Alexandria governorates. Twelve flocks are confirmed
to be infected with IBDV by RT/PCR showing a band of 620 bp on agar gel using a specific
primer of VP2 gene of IBDV. Eight positive PCR samples were chosen for further
characterization by sequencing analysis. Seven isolates were characterized as vvIBDV and one
as classical IBDV. The molecular characterization of isolated IBD field strains in the present
study indicated a continuous circulation of vvIBDV although of intensive vaccination programs.

Key words: IBDV-Chickens-RT/PCR-Sequencing-VP2


 
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Pubmed Style

Nahed A. El-shall, Mahmoud E. Sedeek, Muhammed M. El-badawy, Eid G. Hussein, Ashraf M. Awad. Phylogenetic Characterization of Infectious Bursal Disease (IBD) Viruses Isolated From Field Outbreaks in Chickens From Behera And Alexandria Governorates, Egypt. AJVS. 2018; 56(1): 153-161. doi:10.5455/ajvs.284794


Web Style

Nahed A. El-shall, Mahmoud E. Sedeek, Muhammed M. El-badawy, Eid G. Hussein, Ashraf M. Awad. Phylogenetic Characterization of Infectious Bursal Disease (IBD) Viruses Isolated From Field Outbreaks in Chickens From Behera And Alexandria Governorates, Egypt. http://www.alexjvs.com/?mno=284794 [Access: March 22, 2019]. doi:10.5455/ajvs.284794


AMA (American Medical Association) Style

Nahed A. El-shall, Mahmoud E. Sedeek, Muhammed M. El-badawy, Eid G. Hussein, Ashraf M. Awad. Phylogenetic Characterization of Infectious Bursal Disease (IBD) Viruses Isolated From Field Outbreaks in Chickens From Behera And Alexandria Governorates, Egypt. AJVS. 2018; 56(1): 153-161. doi:10.5455/ajvs.284794



Vancouver/ICMJE Style

Nahed A. El-shall, Mahmoud E. Sedeek, Muhammed M. El-badawy, Eid G. Hussein, Ashraf M. Awad. Phylogenetic Characterization of Infectious Bursal Disease (IBD) Viruses Isolated From Field Outbreaks in Chickens From Behera And Alexandria Governorates, Egypt. AJVS. (2018), [cited March 22, 2019]; 56(1): 153-161. doi:10.5455/ajvs.284794



Harvard Style

Nahed A. El-shall, Mahmoud E. Sedeek, Muhammed M. El-badawy, Eid G. Hussein, Ashraf M. Awad (2018) Phylogenetic Characterization of Infectious Bursal Disease (IBD) Viruses Isolated From Field Outbreaks in Chickens From Behera And Alexandria Governorates, Egypt. AJVS, 56 (1), 153-161. doi:10.5455/ajvs.284794



Turabian Style

Nahed A. El-shall, Mahmoud E. Sedeek, Muhammed M. El-badawy, Eid G. Hussein, Ashraf M. Awad. 2018. Phylogenetic Characterization of Infectious Bursal Disease (IBD) Viruses Isolated From Field Outbreaks in Chickens From Behera And Alexandria Governorates, Egypt. Alexandria Journal of Veterinary Sciences, 56 (1), 153-161. doi:10.5455/ajvs.284794



Chicago Style

Nahed A. El-shall, Mahmoud E. Sedeek, Muhammed M. El-badawy, Eid G. Hussein, Ashraf M. Awad. "Phylogenetic Characterization of Infectious Bursal Disease (IBD) Viruses Isolated From Field Outbreaks in Chickens From Behera And Alexandria Governorates, Egypt." Alexandria Journal of Veterinary Sciences 56 (2018), 153-161. doi:10.5455/ajvs.284794



MLA (The Modern Language Association) Style

Nahed A. El-shall, Mahmoud E. Sedeek, Muhammed M. El-badawy, Eid G. Hussein, Ashraf M. Awad. "Phylogenetic Characterization of Infectious Bursal Disease (IBD) Viruses Isolated From Field Outbreaks in Chickens From Behera And Alexandria Governorates, Egypt." Alexandria Journal of Veterinary Sciences 56.1 (2018), 153-161. Print. doi:10.5455/ajvs.284794



APA (American Psychological Association) Style

Nahed A. El-shall, Mahmoud E. Sedeek, Muhammed M. El-badawy, Eid G. Hussein, Ashraf M. Awad (2018) Phylogenetic Characterization of Infectious Bursal Disease (IBD) Viruses Isolated From Field Outbreaks in Chickens From Behera And Alexandria Governorates, Egypt. Alexandria Journal of Veterinary Sciences, 56 (1), 153-161. doi:10.5455/ajvs.284794





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