E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Research (Original Article) 


Silver Nanoparticles Preparation and their Effect on Full-thickness Skin Wound Healing in Rabbit Model

Somaya A. Amer, Samir R. Nouh, Mahmoud H. Elkammar, Thanaa I. Shalaby, Ahmed S. Korittum.

Abstract
With the increased prevalence of antibiotic-resistant bacteria infections, there is a pressed need for innovative antimicrobial agent. Metal nanoparticle, especially those made of noble metals such as silver, show excellent properties such as signaling the regeneration of collagen, low toxicity in vivo, bacteriostatic and bactericidal activities, and inhibiting the targeting of bacteria in wound. Therefore, this study aims to prepare silver nanoparticles then investigate its antimicrobial effect in wound healing in animal model. Silver nanoparticles were successfully prepared by chemical reduction of silver nitrate, then characterized by UV-Visible absorption spectroscopy. The in-vivo study was done by application of colloidal solution of silver nanoparticles on excisional full- thickness skin defect on the dorsum of New Zeeland rabbit as an animal model. The results indicated significant reduction of the sepsis in two infected cases after the 1st week followed by complete recovery after the 2nd dose of (SNPs drops). While the other cases showed wound closure without any sepsis which demonstrated the high efficiency of silver nanoparticles as antimicrobial agent when compared with the control group which showed persistent signs of sepsis in the all cases. The histological investigation in the control group
proved that the quality of the healing process was very poor. The lengths of the newly formed epithelium, collagen deposition, and the granulation thickness are considered the positive signs of the wound healing process which obviously observed with the animal treated with SNPs.it could be concluded that silver nanoparticles can be used as an effective antimicrobial agent as promising step instead of using systemic the antibiotics.

Key words: Silver nanoparticles, wound healing, rabbit model, anti-microbial agent, characterization.


 
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Pubmed Style

Amer SA, Nouh SR, Elkammar MH, Shalaby TI, Korittum AS. Silver Nanoparticles Preparation and their Effect on Full-thickness Skin Wound Healing in Rabbit Model. AJVS. 2018; 57(2): 79-86. doi:10.5455/ajvs.286067


Web Style

Amer SA, Nouh SR, Elkammar MH, Shalaby TI, Korittum AS. Silver Nanoparticles Preparation and their Effect on Full-thickness Skin Wound Healing in Rabbit Model. http://www.alexjvs.com/?mno=286067 [Access: October 15, 2018]. doi:10.5455/ajvs.286067


AMA (American Medical Association) Style

Amer SA, Nouh SR, Elkammar MH, Shalaby TI, Korittum AS. Silver Nanoparticles Preparation and their Effect on Full-thickness Skin Wound Healing in Rabbit Model. AJVS. 2018; 57(2): 79-86. doi:10.5455/ajvs.286067



Vancouver/ICMJE Style

Amer SA, Nouh SR, Elkammar MH, Shalaby TI, Korittum AS. Silver Nanoparticles Preparation and their Effect on Full-thickness Skin Wound Healing in Rabbit Model. AJVS. (2018), [cited October 15, 2018]; 57(2): 79-86. doi:10.5455/ajvs.286067



Harvard Style

Amer, S. A., Nouh, S. R., Elkammar, M. H., Shalaby, T. I. & Korittum, A. S. (2018) Silver Nanoparticles Preparation and their Effect on Full-thickness Skin Wound Healing in Rabbit Model. AJVS, 57 (2), 79-86. doi:10.5455/ajvs.286067



Turabian Style

Amer, Somaya A., Samir R. Nouh, Mahmoud H. Elkammar, Thanaa I. Shalaby, and Ahmed S. Korittum. 2018. Silver Nanoparticles Preparation and their Effect on Full-thickness Skin Wound Healing in Rabbit Model. Alexandria Journal of Veterinary Sciences, 57 (2), 79-86. doi:10.5455/ajvs.286067



Chicago Style

Amer, Somaya A., Samir R. Nouh, Mahmoud H. Elkammar, Thanaa I. Shalaby, and Ahmed S. Korittum. "Silver Nanoparticles Preparation and their Effect on Full-thickness Skin Wound Healing in Rabbit Model." Alexandria Journal of Veterinary Sciences 57 (2018), 79-86. doi:10.5455/ajvs.286067



MLA (The Modern Language Association) Style

Amer, Somaya A., Samir R. Nouh, Mahmoud H. Elkammar, Thanaa I. Shalaby, and Ahmed S. Korittum. "Silver Nanoparticles Preparation and their Effect on Full-thickness Skin Wound Healing in Rabbit Model." Alexandria Journal of Veterinary Sciences 57.2 (2018), 79-86. Print. doi:10.5455/ajvs.286067



APA (American Psychological Association) Style

Amer, S. A., Nouh, S. R., Elkammar, M. H., Shalaby, T. I. & Korittum, A. S. (2018) Silver Nanoparticles Preparation and their Effect on Full-thickness Skin Wound Healing in Rabbit Model. Alexandria Journal of Veterinary Sciences, 57 (2), 79-86. doi:10.5455/ajvs.286067





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