E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Research (Original Article) 


Evaluation of Extraction Procedures Efficiency for Determination Of Aflatoxins B1 and M1 in Raw Cow’s Milk Collected from Dairy Farms in Qassim Region, Saudi Arabia

Bassam Alharbi, Abdullah Alsayeqh, Abdelmajed Draz, Shawkat Fat’hi.

Abstract
A comparison between the efficiency of the solid-phase extraction (SPE) and dispersive-solid phase extraction (d-SPE) was conducted using ultra-high-performance liquid chromatography equipped with tandem mass spectrometry UPLC-MS/MS, in order to have a simple and sensitive method for the simultaneous determination of aflatoxin B1 (AFB1) and aflatoxin M1 (AFM1) in cow’s milk samples. This method was used to analyze 61 of cow’s milk samples collected from different dairy farms in Qassim region, Saudi Arabia. It was found that the concentrations of AFM1 in 5 (8.2 %) samples were higher than the International levels as recommended by European Commission (EC, 2006). Seven (11.5%) of the samples were contaminated by AFM1 with a concentration lower than the international limits, while only one sample (1.6%) was contaminated with AFB1. The d-SPE procedure has many advantages, such as high recovery and lower value of Limit of Quantitation (LOQ). Therefore, the d-SPE procedure is recommended for analyzing the AFB1 and AFM1 in milk.

Key words: Aflatoxins,dispersive-solid phase extraction, Qassim region, Cow’s milk


 
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Pubmed Style

Alharbi B, Alsayeqh A, Draz A, Fat'hi S. Evaluation of Extraction Procedures Efficiency for Determination Of Aflatoxins B1 and M1 in Raw Cow’s Milk Collected from Dairy Farms in Qassim Region, Saudi Arabia. AJVS. 2019; 60(2): 34-41. doi:10.5455/ajvs.29439


Web Style

Alharbi B, Alsayeqh A, Draz A, Fat'hi S. Evaluation of Extraction Procedures Efficiency for Determination Of Aflatoxins B1 and M1 in Raw Cow’s Milk Collected from Dairy Farms in Qassim Region, Saudi Arabia. http://www.alexjvs.com/?mno=29439 [Access: December 13, 2019]. doi:10.5455/ajvs.29439


AMA (American Medical Association) Style

Alharbi B, Alsayeqh A, Draz A, Fat'hi S. Evaluation of Extraction Procedures Efficiency for Determination Of Aflatoxins B1 and M1 in Raw Cow’s Milk Collected from Dairy Farms in Qassim Region, Saudi Arabia. AJVS. 2019; 60(2): 34-41. doi:10.5455/ajvs.29439



Vancouver/ICMJE Style

Alharbi B, Alsayeqh A, Draz A, Fat'hi S. Evaluation of Extraction Procedures Efficiency for Determination Of Aflatoxins B1 and M1 in Raw Cow’s Milk Collected from Dairy Farms in Qassim Region, Saudi Arabia. AJVS. (2019), [cited December 13, 2019]; 60(2): 34-41. doi:10.5455/ajvs.29439



Harvard Style

Alharbi, B., Alsayeqh, . A., Draz, . A. & Fat'hi, . S. (2019) Evaluation of Extraction Procedures Efficiency for Determination Of Aflatoxins B1 and M1 in Raw Cow’s Milk Collected from Dairy Farms in Qassim Region, Saudi Arabia. AJVS, 60 (2), 34-41. doi:10.5455/ajvs.29439



Turabian Style

Alharbi, Bassam, Abdullah Alsayeqh, Abdelmajed Draz, and Shawkat Fat'hi. 2019. Evaluation of Extraction Procedures Efficiency for Determination Of Aflatoxins B1 and M1 in Raw Cow’s Milk Collected from Dairy Farms in Qassim Region, Saudi Arabia. Alexandria Journal of Veterinary Sciences, 60 (2), 34-41. doi:10.5455/ajvs.29439



Chicago Style

Alharbi, Bassam, Abdullah Alsayeqh, Abdelmajed Draz, and Shawkat Fat'hi. "Evaluation of Extraction Procedures Efficiency for Determination Of Aflatoxins B1 and M1 in Raw Cow’s Milk Collected from Dairy Farms in Qassim Region, Saudi Arabia." Alexandria Journal of Veterinary Sciences 60 (2019), 34-41. doi:10.5455/ajvs.29439



MLA (The Modern Language Association) Style

Alharbi, Bassam, Abdullah Alsayeqh, Abdelmajed Draz, and Shawkat Fat'hi. "Evaluation of Extraction Procedures Efficiency for Determination Of Aflatoxins B1 and M1 in Raw Cow’s Milk Collected from Dairy Farms in Qassim Region, Saudi Arabia." Alexandria Journal of Veterinary Sciences 60.2 (2019), 34-41. Print. doi:10.5455/ajvs.29439



APA (American Psychological Association) Style

Alharbi, B., Alsayeqh, . A., Draz, . A. & Fat'hi, . S. (2019) Evaluation of Extraction Procedures Efficiency for Determination Of Aflatoxins B1 and M1 in Raw Cow’s Milk Collected from Dairy Farms in Qassim Region, Saudi Arabia. Alexandria Journal of Veterinary Sciences, 60 (2), 34-41. doi:10.5455/ajvs.29439





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