E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Research (Original Article) 


Prevalence of Staphylococcus aureus In Broiler Chickens with Special Reference to Beta-Lactam Resistance Genes in the Isolated Strains

Aml A. Bakheet, Omar Amen, Sayed H.AL. Habaty, Samah F. Darwish.

Abstract
The dramatic increase in the prevalence of antimicrobial-resistant Staphylococcus aureus (S. aureus) is receiving wide spread attention, due to multi-resistant strains, diminishing the usefulness of antibiotics in human and animal medicine and, thereby limiting therapeutic options. In this study, 250 samples of chicken swabs were collected from five different poultry farms (50 samples from each farm) within Upper Egypt area. 157 staphylococci strains were detected with an isolation rate of (62.8%). isolates of S. aureus were confirmed using standard microbiological methods. Eighty one (55.9%) were coagulase positive staphylococci, and 76(48.4%) were coagulase negative staphylococci, phenotypic prediction of ╬▓-lactam resistance among the 81 isolated coagulase positive staphylococci was declared using disk diffusion test against, cefoxitin and oxicillin which were used for detection of mecA mediated ╬▓-lactam resistance (methicillin resistance) while penicillin and amoxicillin/clavulinic acid (AMC) antibiotic discs were used to monitor blaZ mediated resistance. Out of 81 isolates ,all isolates were 100% resist to oxacillin and 93.8 % were resist to cefoxitin, while 90.1 % were resist to penicillin and 87.6% were resist to AMC. The antibiotic susceptibility results were judged by PCR, the mecA ,and blaZ genes were tested for the evaluation of the susceptibility of coagulase positive Staphylococci against oxacillin and ▀-lactams, the thirty one reprsentative coagulase positive staphylococci isolates confirmed that all the isolates were Staphylococci by successful amplification of the 228 bp PCR product of the Staphylococcal specific 16S rRNA gene. While , 20 isolates were confirmed to be S.aureus by successful amplification of 279 bp PCR product of the S.aureus specific thermonuclease gene .On using the same multiplex PCR assay, detection of blaZ and/or mecA genes were performed through successful amplification of 517 bp and /or 147 bp specific products, respectively. Out of the 31 reprsentative coagulase positive Staphylococci isolated from broiler chickens, 20 (64.5%) were mecA gene positive while 23 (74%) were ╬▓- blaZ gene positive. Also, 15 (48.3%) of the isolates carried both mecA and blaZ genes while only 3 isolates (9.6%) were negative for both genes. Concerning MIC of 12 representative coagulase positive staphylococci isolates against 12 selected antibiotics commonly used in poultry farms, 100% of isolates were resistant to streptomycin, spectinomycin ,ampicillin, Cefotax, Trimethoprim-sulphamethazole, gentamycin, and Methicillin with MIC >128, while 100% of isolates were sensitive to tylosin, lincomycine, enrofloxacin, ciprofloxacin and oxytetracyclin.

Key words: Staphylococcus aureus, broiler chickens, PCR , mecA, blaZ genes


 
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Pubmed Style

Bakheet AA, Amen O, Habaty SH, Darwish SF. Prevalence of Staphylococcus aureus In Broiler Chickens with Special Reference to Beta-Lactam Resistance Genes in the Isolated Strains. AJVS. 2018; 57(2): 25-33. doi:10.5455/ajvs.297627


Web Style

Bakheet AA, Amen O, Habaty SH, Darwish SF. Prevalence of Staphylococcus aureus In Broiler Chickens with Special Reference to Beta-Lactam Resistance Genes in the Isolated Strains. http://www.alexjvs.com/?mno=297627 [Access: August 21, 2018]. doi:10.5455/ajvs.297627


AMA (American Medical Association) Style

Bakheet AA, Amen O, Habaty SH, Darwish SF. Prevalence of Staphylococcus aureus In Broiler Chickens with Special Reference to Beta-Lactam Resistance Genes in the Isolated Strains. AJVS. 2018; 57(2): 25-33. doi:10.5455/ajvs.297627



Vancouver/ICMJE Style

Bakheet AA, Amen O, Habaty SH, Darwish SF. Prevalence of Staphylococcus aureus In Broiler Chickens with Special Reference to Beta-Lactam Resistance Genes in the Isolated Strains. AJVS. (2018), [cited August 21, 2018]; 57(2): 25-33. doi:10.5455/ajvs.297627



Harvard Style

Bakheet, A. A., Amen, O., Habaty, S. H. & Darwish, S. F. (2018) Prevalence of Staphylococcus aureus In Broiler Chickens with Special Reference to Beta-Lactam Resistance Genes in the Isolated Strains. AJVS, 57 (2), 25-33. doi:10.5455/ajvs.297627



Turabian Style

Bakheet, Aml A., Omar Amen, Sayed H.AL. Habaty, and Samah F. Darwish. 2018. Prevalence of Staphylococcus aureus In Broiler Chickens with Special Reference to Beta-Lactam Resistance Genes in the Isolated Strains. Alexandria Journal of Veterinary Sciences, 57 (2), 25-33. doi:10.5455/ajvs.297627



Chicago Style

Bakheet, Aml A., Omar Amen, Sayed H.AL. Habaty, and Samah F. Darwish. "Prevalence of Staphylococcus aureus In Broiler Chickens with Special Reference to Beta-Lactam Resistance Genes in the Isolated Strains." Alexandria Journal of Veterinary Sciences 57 (2018), 25-33. doi:10.5455/ajvs.297627



MLA (The Modern Language Association) Style

Bakheet, Aml A., Omar Amen, Sayed H.AL. Habaty, and Samah F. Darwish. "Prevalence of Staphylococcus aureus In Broiler Chickens with Special Reference to Beta-Lactam Resistance Genes in the Isolated Strains." Alexandria Journal of Veterinary Sciences 57.2 (2018), 25-33. Print. doi:10.5455/ajvs.297627



APA (American Psychological Association) Style

Bakheet, A. A., Amen, O., Habaty, S. H. & Darwish, S. F. (2018) Prevalence of Staphylococcus aureus In Broiler Chickens with Special Reference to Beta-Lactam Resistance Genes in the Isolated Strains. Alexandria Journal of Veterinary Sciences, 57 (2), 25-33. doi:10.5455/ajvs.297627





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