E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Research (Original Article) 


Molecular Characterization of Two Selected Pigeon Paramyxovirus-1 Isolates Reveals Two Different Cleavage Site Amino Acid Motifs

Asmaa Magouz, Ali Etman, Abdelnaby Metwally, Gabr Elbagoury, Abdelrazek Desouky.

Abstract
The aim of this study was the isolation, identification and molecular characterization of Avian (Pigeon) Paramyxovirus-1 (APMV-1) isolated from clinically affected pigeons suspected to be infected with PPMV-1 in Egypt between 2016 and 2017. Twenty-five field samples were collected and inoculated into the allantoic cavity of ECE. Allantoic fluids were tested for haemagglutinating (HA) activity followed by haemagglutination inhibition (HI) test for virus identification. Molecular confirmation was done by reverse transcription polymerase chain reaction (RT-PCR) using primers specific to the fusion (F) gene. Results revealed 12 out of 25 positive samples. Two samples were selected for sequencing and phylogenetic analysis which revealed that two different amino acid motifs were found at the cleavage site of F protein: 112 KRQKRF117 (associated with virulent strains) and 112GRQGRL117 (associated with lentogenic strains).To our knowledge, this is the first reported PPMV-1 isolates that possess the sequences of 112GRQGRL117 within the F0 protein.

Key words: Pigeon Paramyxovirus-1, F gene, Phylogenetic analysis, Amino acid motifs


 
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Pubmed Style

Magouz A, Etman A, Metwally A, Elbagoury G, Desouky A. Molecular Characterization of Two Selected Pigeon Paramyxovirus-1 Isolates Reveals Two Different Cleavage Site Amino Acid Motifs. AJVS. 2018; 59(1): 1-10. doi:10.5455/ajvs.302643191


Web Style

Magouz A, Etman A, Metwally A, Elbagoury G, Desouky A. Molecular Characterization of Two Selected Pigeon Paramyxovirus-1 Isolates Reveals Two Different Cleavage Site Amino Acid Motifs. http://www.alexjvs.com/?mno=302643191 [Access: November 17, 2018]. doi:10.5455/ajvs.302643191


AMA (American Medical Association) Style

Magouz A, Etman A, Metwally A, Elbagoury G, Desouky A. Molecular Characterization of Two Selected Pigeon Paramyxovirus-1 Isolates Reveals Two Different Cleavage Site Amino Acid Motifs. AJVS. 2018; 59(1): 1-10. doi:10.5455/ajvs.302643191



Vancouver/ICMJE Style

Magouz A, Etman A, Metwally A, Elbagoury G, Desouky A. Molecular Characterization of Two Selected Pigeon Paramyxovirus-1 Isolates Reveals Two Different Cleavage Site Amino Acid Motifs. AJVS. (2018), [cited November 17, 2018]; 59(1): 1-10. doi:10.5455/ajvs.302643191



Harvard Style

Magouz, A., Etman, . A., Metwally, . A., Elbagoury, . G. & Desouky, . A. (2018) Molecular Characterization of Two Selected Pigeon Paramyxovirus-1 Isolates Reveals Two Different Cleavage Site Amino Acid Motifs. AJVS, 59 (1), 1-10. doi:10.5455/ajvs.302643191



Turabian Style

Magouz, Asmaa, Ali Etman, Abdelnaby Metwally, Gabr Elbagoury, and Abdelrazek Desouky. 2018. Molecular Characterization of Two Selected Pigeon Paramyxovirus-1 Isolates Reveals Two Different Cleavage Site Amino Acid Motifs. Alexandria Journal of Veterinary Sciences, 59 (1), 1-10. doi:10.5455/ajvs.302643191



Chicago Style

Magouz, Asmaa, Ali Etman, Abdelnaby Metwally, Gabr Elbagoury, and Abdelrazek Desouky. "Molecular Characterization of Two Selected Pigeon Paramyxovirus-1 Isolates Reveals Two Different Cleavage Site Amino Acid Motifs." Alexandria Journal of Veterinary Sciences 59 (2018), 1-10. doi:10.5455/ajvs.302643191



MLA (The Modern Language Association) Style

Magouz, Asmaa, Ali Etman, Abdelnaby Metwally, Gabr Elbagoury, and Abdelrazek Desouky. "Molecular Characterization of Two Selected Pigeon Paramyxovirus-1 Isolates Reveals Two Different Cleavage Site Amino Acid Motifs." Alexandria Journal of Veterinary Sciences 59.1 (2018), 1-10. Print. doi:10.5455/ajvs.302643191



APA (American Psychological Association) Style

Magouz, A., Etman, . A., Metwally, . A., Elbagoury, . G. & Desouky, . A. (2018) Molecular Characterization of Two Selected Pigeon Paramyxovirus-1 Isolates Reveals Two Different Cleavage Site Amino Acid Motifs. Alexandria Journal of Veterinary Sciences, 59 (1), 1-10. doi:10.5455/ajvs.302643191





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