E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Research (Original Article) 


Verifying parentage and genetic variability among Arabian horse using ISSR markers

Rania A. Tahseen, Karam A. Amein, Dalia H. Samak, Yasser S. EL-Sayed, Sherif M. Nasr.

Abstract
The study aimed to verify parentage and genetic variability among twelve Arabian horses using five ISSR markers. Forty-five DNA loci, which extracted for the sire, mare and foal were amplified and replicated using the tested primers with an average of 9.0 loci per primer, and every marker is compared. The total number of polymorphic alleles was 33 (73.33% polymorphism), with an average of 6.6 alleles per primer. The markers (GAG)6G-ISSR (12 loci), (AGC)6C-ISSR (11 loci) and (AGC)6G-ISSR (9 loci) amplified the highest number of loci and possessed the highest PIC values indicating that they are the most promising for analysis of genetic variety and paternity in the Arabian horse. The highest similarity and shortest genetic distance (Si=0.926, Gd=0.077) were between Abeer and her daughter Karma. While, the lowest similarity and longest genetic distance (Si=0.353, Gd=1.041) between Sary and Farhan. The UPGMA cluster analysis supported the genetic relatedness as expected among the 12 Arabian horses. The dendrogram grouped the 12 Arabian horses according to their paternity, i.e. pedigree levels, into three main clusters within a branched-off 0.439 genetic similarity.

Key words: Arabian horse; Paternity testing; DNA polymorphism; ISSR markers


 
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Pubmed Style

Tahseen RA, Amein KA, Samak DH, EL-Sayed YS, Nasr SM. Verifying parentage and genetic variability among Arabian horse using ISSR markers. AJVS. 2018; 59(1): 125-133. doi:10.5455/ajvs.7699


Web Style

Tahseen RA, Amein KA, Samak DH, EL-Sayed YS, Nasr SM. Verifying parentage and genetic variability among Arabian horse using ISSR markers. http://www.alexjvs.com/?mno=7699 [Access: November 17, 2018]. doi:10.5455/ajvs.7699


AMA (American Medical Association) Style

Tahseen RA, Amein KA, Samak DH, EL-Sayed YS, Nasr SM. Verifying parentage and genetic variability among Arabian horse using ISSR markers. AJVS. 2018; 59(1): 125-133. doi:10.5455/ajvs.7699



Vancouver/ICMJE Style

Tahseen RA, Amein KA, Samak DH, EL-Sayed YS, Nasr SM. Verifying parentage and genetic variability among Arabian horse using ISSR markers. AJVS. (2018), [cited November 17, 2018]; 59(1): 125-133. doi:10.5455/ajvs.7699



Harvard Style

Tahseen, R. A., Amein, . K. A., Samak, . D. H., EL-Sayed, . Y. S. & Nasr, . S. M. (2018) Verifying parentage and genetic variability among Arabian horse using ISSR markers. AJVS, 59 (1), 125-133. doi:10.5455/ajvs.7699



Turabian Style

Tahseen, Rania A., Karam A. Amein, Dalia H. Samak, Yasser S. EL-Sayed, and Sherif M. Nasr. 2018. Verifying parentage and genetic variability among Arabian horse using ISSR markers. Alexandria Journal of Veterinary Sciences, 59 (1), 125-133. doi:10.5455/ajvs.7699



Chicago Style

Tahseen, Rania A., Karam A. Amein, Dalia H. Samak, Yasser S. EL-Sayed, and Sherif M. Nasr. "Verifying parentage and genetic variability among Arabian horse using ISSR markers." Alexandria Journal of Veterinary Sciences 59 (2018), 125-133. doi:10.5455/ajvs.7699



MLA (The Modern Language Association) Style

Tahseen, Rania A., Karam A. Amein, Dalia H. Samak, Yasser S. EL-Sayed, and Sherif M. Nasr. "Verifying parentage and genetic variability among Arabian horse using ISSR markers." Alexandria Journal of Veterinary Sciences 59.1 (2018), 125-133. Print. doi:10.5455/ajvs.7699



APA (American Psychological Association) Style

Tahseen, R. A., Amein, . K. A., Samak, . D. H., EL-Sayed, . Y. S. & Nasr, . S. M. (2018) Verifying parentage and genetic variability among Arabian horse using ISSR markers. Alexandria Journal of Veterinary Sciences, 59 (1), 125-133. doi:10.5455/ajvs.7699





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