E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Molecular Identification of Virulence Genes of Staphylococcus Aureus Isolated from Milk Products

Manal S. Elmaghraby, Eman E. Abdeen, Nahla A. Abou-Elros, Abdel Rahman El Bagory.

Abstract
Food intoxication by S.aureus was listed as the third etiology of food intoxication all over the world. This study was conducted to investigate the prevalence rate of S.aureus in skim milk soft cheese( Kariesh cheese) and ice cream samples from local markets in Menoufia province, Egypt. Two-hundred samples (100 for each) were examined bacteriologically for prevalence of Staphylococcus aureus through culturing on specific media and biochemical identification. In addition to the molecular characterization of some virulence and antibiotic resistance genes in isolated strains. The obtained results revealed that the prevalence rate of S. aureus was 24% in kariesh cheese with an average count of 1.22x 103 1.39 x 102 CFU/g and 16% in ice-cream samples with an average count of 1.04 x103 ± 8.89x10 CFU/ml .Furthermore, the molecular characterization using specific primers sets for detection of Coa, Spa and blaZ genes of S. aureus was performed by amplification of these primers at single amplicon at 630 bp, 226 bp, and 173bp respectively. The molecular results confirmed the detection of these genes in all tested isolates of S.aureus. The obtained results proved that PCR technique is more sensitive and specific in detection of these genes than other conventional techniques and help to understanding the role of these genes in pathogenicity pathway in different disease conditions and construction of effective control measure.

Key words: S.aureus, food, PCR, Coa, SpA, blaz genes.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Manal S. Elmaghraby
Articles by Eman E. Abdeen
Articles by Nahla A. Abou-Elros
Articles by Abdel Rahman El Bagory
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Elmaghraby MS, Abdeen EE, Abou-Elros NA, Bagory ARE. Molecular Identification of Virulence Genes of Staphylococcus Aureus Isolated from Milk Products. AJVS. 2018; 58(1): 57-64. doi:10.5455/ajvs.299828


Web Style

Elmaghraby MS, Abdeen EE, Abou-Elros NA, Bagory ARE. Molecular Identification of Virulence Genes of Staphylococcus Aureus Isolated from Milk Products. https://www.alexjvs.com/?mno=299828 [Access: December 03, 2023]. doi:10.5455/ajvs.299828


AMA (American Medical Association) Style

Elmaghraby MS, Abdeen EE, Abou-Elros NA, Bagory ARE. Molecular Identification of Virulence Genes of Staphylococcus Aureus Isolated from Milk Products. AJVS. 2018; 58(1): 57-64. doi:10.5455/ajvs.299828



Vancouver/ICMJE Style

Elmaghraby MS, Abdeen EE, Abou-Elros NA, Bagory ARE. Molecular Identification of Virulence Genes of Staphylococcus Aureus Isolated from Milk Products. AJVS. (2018), [cited December 03, 2023]; 58(1): 57-64. doi:10.5455/ajvs.299828



Harvard Style

Elmaghraby, M. S., Abdeen, . E. E., Abou-Elros, . N. A. & Bagory, . A. R. E. (2018) Molecular Identification of Virulence Genes of Staphylococcus Aureus Isolated from Milk Products. AJVS, 58 (1), 57-64. doi:10.5455/ajvs.299828



Turabian Style

Elmaghraby, Manal S., Eman E. Abdeen, Nahla A. Abou-Elros, and Abdel Rahman El Bagory. 2018. Molecular Identification of Virulence Genes of Staphylococcus Aureus Isolated from Milk Products. Alexandria Journal of Veterinary Sciences, 58 (1), 57-64. doi:10.5455/ajvs.299828



Chicago Style

Elmaghraby, Manal S., Eman E. Abdeen, Nahla A. Abou-Elros, and Abdel Rahman El Bagory. "Molecular Identification of Virulence Genes of Staphylococcus Aureus Isolated from Milk Products." Alexandria Journal of Veterinary Sciences 58 (2018), 57-64. doi:10.5455/ajvs.299828



MLA (The Modern Language Association) Style

Elmaghraby, Manal S., Eman E. Abdeen, Nahla A. Abou-Elros, and Abdel Rahman El Bagory. "Molecular Identification of Virulence Genes of Staphylococcus Aureus Isolated from Milk Products." Alexandria Journal of Veterinary Sciences 58.1 (2018), 57-64. Print. doi:10.5455/ajvs.299828



APA (American Psychological Association) Style

Elmaghraby, M. S., Abdeen, . E. E., Abou-Elros, . N. A. & Bagory, . A. R. E. (2018) Molecular Identification of Virulence Genes of Staphylococcus Aureus Isolated from Milk Products. Alexandria Journal of Veterinary Sciences, 58 (1), 57-64. doi:10.5455/ajvs.299828





Most Viewed Articles
Most Accessed Articles

  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Most Downloaded
    Top Downloaded Articles

  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Effect of Excess Fluoride on Reproductive Potentials in Farm Animals (Ovine)
    Ghada H. Abdel-Rahman, Hanaa A. El-Hallawany, Dohreig RA.
    AJVS. 2018; 57(2): 41-57
    » Abstract » doi: 10.5455/ajvs.296364

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract » doi: 10.5455/ajvs.47290

  • Most Cited Articles
    Most Cited Articles