E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Molecular characterization of vibrio species isolated from fish using PCR technique

Eslam A. Asran, Samy A. Khalil, Yasr N. Awad, Mona M. Hussein.

Abstract
A total of 100 fish samples (Oreochromis niloticus) were collected as 25 fish apparently health and 75 fish appeared to be clinically diseased fish. Samples of kidney, heart, liver, gills and skin lesions are collected and cultured on TCBS medium. Eighty three samples show biochemically positive to vibrio species.10 random samples amplified to 16srRNA gene by PCR technique for more accurate identification resulting in 8 isolates were positive to 16srRNA.The 8 isolates amplified to suspected species specific primers. Resulted in 4 V.parahaemolyticus (50%), 2 V. harveyi (25%) ,1 V. anguillarum (12.5%) and 1 V. alginolyticus (12.5%). Each species amplified to PCR technique for more identification to virulence genes. V.parahaemolyticus amplified to tdh and trh; V. harveyi to Partial hly and Vhh; V. alginolyticus amplified to trh and tdh and V.anguillarum amplified to virA and angM. This study proved that PCR protocols could be useful tools for rapid and simultaneous detection of different Vibrio species virulence factors. Also using of 16srRNA gene in molecular characterization (PCR) of Vibrio is the fastest and most accurate method to identify all isolates of Vibrio.

Key words: Vibriosis, vibrio species, PCR, 16srRNA and Virulence factors.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Eslam A. Asran
Articles by Samy A. Khalil
Articles by Yasr N. Awad
Articles by Mona M. Hussein
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Asran EA, Khalil SA, Awad YN, Hussein MM. Molecular characterization of vibrio species isolated from fish using PCR technique. AJVS. 2020; 67(2): 9-19. doi:10.5455/ajvs.117363


Web Style

Asran EA, Khalil SA, Awad YN, Hussein MM. Molecular characterization of vibrio species isolated from fish using PCR technique. https://www.alexjvs.com/?mno=117363 [Access: December 03, 2023]. doi:10.5455/ajvs.117363


AMA (American Medical Association) Style

Asran EA, Khalil SA, Awad YN, Hussein MM. Molecular characterization of vibrio species isolated from fish using PCR technique. AJVS. 2020; 67(2): 9-19. doi:10.5455/ajvs.117363



Vancouver/ICMJE Style

Asran EA, Khalil SA, Awad YN, Hussein MM. Molecular characterization of vibrio species isolated from fish using PCR technique. AJVS. (2020), [cited December 03, 2023]; 67(2): 9-19. doi:10.5455/ajvs.117363



Harvard Style

Asran, E. A., Khalil, . S. A., Awad, . Y. N. & Hussein, . M. M. (2020) Molecular characterization of vibrio species isolated from fish using PCR technique. AJVS, 67 (2), 9-19. doi:10.5455/ajvs.117363



Turabian Style

Asran, Eslam A., Samy A. Khalil, Yasr N. Awad, and Mona M. Hussein. 2020. Molecular characterization of vibrio species isolated from fish using PCR technique. Alexandria Journal of Veterinary Sciences, 67 (2), 9-19. doi:10.5455/ajvs.117363



Chicago Style

Asran, Eslam A., Samy A. Khalil, Yasr N. Awad, and Mona M. Hussein. "Molecular characterization of vibrio species isolated from fish using PCR technique." Alexandria Journal of Veterinary Sciences 67 (2020), 9-19. doi:10.5455/ajvs.117363



MLA (The Modern Language Association) Style

Asran, Eslam A., Samy A. Khalil, Yasr N. Awad, and Mona M. Hussein. "Molecular characterization of vibrio species isolated from fish using PCR technique." Alexandria Journal of Veterinary Sciences 67.2 (2020), 9-19. Print. doi:10.5455/ajvs.117363



APA (American Psychological Association) Style

Asran, E. A., Khalil, . S. A., Awad, . Y. N. & Hussein, . M. M. (2020) Molecular characterization of vibrio species isolated from fish using PCR technique. Alexandria Journal of Veterinary Sciences, 67 (2), 9-19. doi:10.5455/ajvs.117363





Most Viewed Articles
Most Accessed Articles

  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Most Downloaded
    Top Downloaded Articles

  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Effect of Excess Fluoride on Reproductive Potentials in Farm Animals (Ovine)
    Ghada H. Abdel-Rahman, Hanaa A. El-Hallawany, Dohreig RA.
    AJVS. 2018; 57(2): 41-57
    » Abstract » doi: 10.5455/ajvs.296364

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract » doi: 10.5455/ajvs.47290

  • Most Cited Articles
    Most Cited Articles