Genetic Detection of Listeria monocytogenes Recovered from Fillet Fish Samples
Ola H. Harb, Gehad A. Fath-Elbab, Reyad R. Shawish, Walid S. Mousa, Eman E. Abdeen. | | | | Abstract Listeria monocytogenes is categorized a major foodborne pathogen responsible for number of serious diseases in both human and veterinary field. The current study was carried out to spotlight on the bacteriological and genetic detection of L.monocytogenes obtained from fillet fish samples. A total of one-hundred fillet fish samples were collected from Menoufia governorate and subjected to bacteriological analysis onto three specific and selective medium. The over prevalence rate was 7% (7/100). The typical colony characters of L.monocytogenes on Oxford medium appear as colonies of gray and or black color encompassed with a black halo, while, on CHROMagar medium, it give blue/green colonies. On blood ager medium it exhibit narrow zone of β hemolysis. The use of recent modern molecular techniques such as mPCR was detected as effective alternative approach for the identification of 16SrRNA of L.monocytogenes at 938 bp and efficient amplification the hlyA gene at 702 bp. In conclusion, this study demonstrated the importance of 16SrRNA for specific identification L.monocytogenes in fillet fish samples and recommended the importance of excellent quality cooking for food particular fillet fish to avoid infection with L. monocytogenes for human consumers. Key words: L.monocytogenes, fillet fish, mPCR, hlyA gene
| |
|
Most Viewed Articles
Most Accessed Articles Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib AJVS. 2013; 39(1): 82-90 » Abstract
Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed AJVS. 2014; 40(1): 29-43 » Abstract » doi: 10.5455/ajvs.48333
Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda AJVS. 2014; 41(1): 19-27 » Abstract » doi: 10.5455/ajvs.154650
Molecular Characterization of Listeria Species Isolated from Frozen Fish Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy AJVS. 2014; 40(1): 1-15 » Abstract » doi: 10.5455/ajvs.45443
Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie AJVS. 2014; 40(1): 95-111 » Abstract » doi: 10.5455/ajvs.48232
Most Downloaded
Top Downloaded Articles Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky AJVS. 2016; 51(2): 367-373 » Abstract
Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie AJVS. 2014; 40(1): 95-111 » Abstract » doi: 10.5455/ajvs.48232
Effect of Excess Fluoride on Reproductive Potentials in Farm Animals (Ovine) Ghada H. Abdel-Rahman, Hanaa A. El-Hallawany, Dohreig RA. AJVS. 2018; 57(2): 41-57 » Abstract » doi: 10.5455/ajvs.296364
Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed AJVS. 2014; 40(1): 29-43 » Abstract » doi: 10.5455/ajvs.48333
Using of Polypropylene Mesh for Hernioplasty in Calves Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed AJVS. 2014; 40(1): 112-117 » Abstract » doi: 10.5455/ajvs.47290
Most Cited Articles
Most Cited Articles
|