E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Molecular Characterization of Yersinia Enterocolitica Isolated From Chicken Meat Samples

Safaa M. Shabana, Samy A. Khalil, Abd-El-Halem M. Hegazy.

Abstract
The present study was carried out for identification and molecular analysis of Yersinia enterocolitica isolated from fresh raw chicken meat samples. A total of 114 chicken meat samples were collected randomly from different chicken shops in the Kafr El- Sheikh city and tested by culturing and PCR methods. Twenty isolates out of 114 fresh raw chicken meat samples (17.5%) were found to be positive for Yersinia enterocolitica. Out of 57 chicken breast muscle samples 12 were positive (10.5% ) and in the same time only 8 positive samples detected in 57 chicken thigh muscle samples (7%) for isolation of Yersinia enterocolitica. A PCR-based assay was developed to detect the prevalence of Yersinia enterocolitica in chicken meat samples and to evaluate plasmid- and chromosome-borne virulence genes. The result of PCR examination revealed that, only 8 isolates out of 12 randomly selected isolates (66.6%) were positive as Yersinia enterocolitica by the specific gene of Yersinia enterocolitica (16S ribosomal RNA ). The results of detection of the virulence genes (ail, ystA and virF) were 75%, 50% and 37.5% respectively. These virulence genes were not detected in 2 examined isolates. The yadA gene was not detected in any of the 8 examined isolates by multiplex PCR technique. This study highlighted the importance of chicken meat as potential sources of pathogenic Yersinia enterocolitica.

Key words: Yersinia enterocolitica, chicken meat, PCR techniques, virulence genes


 
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Pubmed Style

Safaa M. Shabana, Samy A. Khalil, Abd-El-Halem M. Hegazy. Molecular Characterization of Yersinia Enterocolitica Isolated From Chicken Meat Samples. AJVS. 2015; 46(1): 124-129. doi:10.5455/ajvs.184190


Web Style

Safaa M. Shabana, Samy A. Khalil, Abd-El-Halem M. Hegazy. Molecular Characterization of Yersinia Enterocolitica Isolated From Chicken Meat Samples. https://www.alexjvs.com/?mno=184190 [Access: December 03, 2023]. doi:10.5455/ajvs.184190


AMA (American Medical Association) Style

Safaa M. Shabana, Samy A. Khalil, Abd-El-Halem M. Hegazy. Molecular Characterization of Yersinia Enterocolitica Isolated From Chicken Meat Samples. AJVS. 2015; 46(1): 124-129. doi:10.5455/ajvs.184190



Vancouver/ICMJE Style

Safaa M. Shabana, Samy A. Khalil, Abd-El-Halem M. Hegazy. Molecular Characterization of Yersinia Enterocolitica Isolated From Chicken Meat Samples. AJVS. (2015), [cited December 03, 2023]; 46(1): 124-129. doi:10.5455/ajvs.184190



Harvard Style

Safaa M. Shabana, Samy A. Khalil, Abd-El-Halem M. Hegazy (2015) Molecular Characterization of Yersinia Enterocolitica Isolated From Chicken Meat Samples. AJVS, 46 (1), 124-129. doi:10.5455/ajvs.184190



Turabian Style

Safaa M. Shabana, Samy A. Khalil, Abd-El-Halem M. Hegazy. 2015. Molecular Characterization of Yersinia Enterocolitica Isolated From Chicken Meat Samples. Alexandria Journal of Veterinary Sciences, 46 (1), 124-129. doi:10.5455/ajvs.184190



Chicago Style

Safaa M. Shabana, Samy A. Khalil, Abd-El-Halem M. Hegazy. "Molecular Characterization of Yersinia Enterocolitica Isolated From Chicken Meat Samples." Alexandria Journal of Veterinary Sciences 46 (2015), 124-129. doi:10.5455/ajvs.184190



MLA (The Modern Language Association) Style

Safaa M. Shabana, Samy A. Khalil, Abd-El-Halem M. Hegazy. "Molecular Characterization of Yersinia Enterocolitica Isolated From Chicken Meat Samples." Alexandria Journal of Veterinary Sciences 46.1 (2015), 124-129. Print. doi:10.5455/ajvs.184190



APA (American Psychological Association) Style

Safaa M. Shabana, Samy A. Khalil, Abd-El-Halem M. Hegazy (2015) Molecular Characterization of Yersinia Enterocolitica Isolated From Chicken Meat Samples. Alexandria Journal of Veterinary Sciences, 46 (1), 124-129. doi:10.5455/ajvs.184190





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