E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Association of Hsp70 Gene Polymorphism And Bull Semen Quality In Winter And Summer Seasons

Jehan A. Gafer, Ghada H. Abd El-Rahman, Rawash Z. M..

Abstract
Objectives of current study were to identify single nucleotide polymorphisms (SNP) in heat shock protein70 (Hsp70) promoter gene of bulls (cattle and buffalo) and its association with semen quality traits and some enzymatic, hormonal and lipid parameters during winter and summer seasons. Semen samples of 37 animals (Cattle n=21 and buffalo n=16), were used in the study. Biochemical Parameters and hormonal profile were estimated. Qualities of fresh semen and frozen-thaw semen were evaluated for motility, plasma membrane and acrosome integrities. PCR-SSCP was used to detect SNPs and different patterns were sequenced. The study demonstrate all the measured biochemical parameters and hormonal profile of cattle and buffalo were significantly different (P<0.05) in winter as compared to summer. Results of PCR-SSCP and sequencing detected a new synonymous SNP 812 T>C transition was the same in both species and could clear two genotypes TT and TC. Association analysis revealed significance (P<0.001) with fresh and some of frozen-thawed semen traits in cattle population and T allele seemed to be the favorable allele. Regarding to buffalo population different alleles has no significance on any of semen traits. By study the association with biochemical and hormonal parameters there were no significance except for T3 in buffalo population and C allele appeared to be the favorable allele for T3 level even in summer season. This the first study concerned with the association of Hsp70 polymorphisms and semen quality, biochemical and hormonal parameters of semen in winter and summer seasons in buffalo population. Conclusion: High temperature has negative impact on biochemical and hormonal parameter consequently semen quality. Biochemical and hormonal profile can be used as good indicator for semen quality. polymorphisms in HSP70 gene can affect semen quality and suggest to be used in artificial insemination programs as marker assisted selection for anti-heat stress and good reproductive bulls.

Key words: HSP70, Polymorphism, Biochemical parameter, Hormonal profile, Semen quality


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Jehan A. Gafer
Articles by Ghada H. Abd El-Rahman
Articles by Rawash Z. M.
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Jehan A. Gafer, Ghada H. Abd El-Rahman, Rawash Z. M.. Association of Hsp70 Gene Polymorphism And Bull Semen Quality In Winter And Summer Seasons. AJVS. 2015; 46(1): 1-14. doi:10.5455/ajvs.186038


Web Style

Jehan A. Gafer, Ghada H. Abd El-Rahman, Rawash Z. M.. Association of Hsp70 Gene Polymorphism And Bull Semen Quality In Winter And Summer Seasons. https://www.alexjvs.com/?mno=186038 [Access: December 04, 2023]. doi:10.5455/ajvs.186038


AMA (American Medical Association) Style

Jehan A. Gafer, Ghada H. Abd El-Rahman, Rawash Z. M.. Association of Hsp70 Gene Polymorphism And Bull Semen Quality In Winter And Summer Seasons. AJVS. 2015; 46(1): 1-14. doi:10.5455/ajvs.186038



Vancouver/ICMJE Style

Jehan A. Gafer, Ghada H. Abd El-Rahman, Rawash Z. M.. Association of Hsp70 Gene Polymorphism And Bull Semen Quality In Winter And Summer Seasons. AJVS. (2015), [cited December 04, 2023]; 46(1): 1-14. doi:10.5455/ajvs.186038



Harvard Style

Jehan A. Gafer, Ghada H. Abd El-Rahman, Rawash Z. M. (2015) Association of Hsp70 Gene Polymorphism And Bull Semen Quality In Winter And Summer Seasons. AJVS, 46 (1), 1-14. doi:10.5455/ajvs.186038



Turabian Style

Jehan A. Gafer, Ghada H. Abd El-Rahman, Rawash Z. M.. 2015. Association of Hsp70 Gene Polymorphism And Bull Semen Quality In Winter And Summer Seasons. Alexandria Journal of Veterinary Sciences, 46 (1), 1-14. doi:10.5455/ajvs.186038



Chicago Style

Jehan A. Gafer, Ghada H. Abd El-Rahman, Rawash Z. M.. "Association of Hsp70 Gene Polymorphism And Bull Semen Quality In Winter And Summer Seasons." Alexandria Journal of Veterinary Sciences 46 (2015), 1-14. doi:10.5455/ajvs.186038



MLA (The Modern Language Association) Style

Jehan A. Gafer, Ghada H. Abd El-Rahman, Rawash Z. M.. "Association of Hsp70 Gene Polymorphism And Bull Semen Quality In Winter And Summer Seasons." Alexandria Journal of Veterinary Sciences 46.1 (2015), 1-14. Print. doi:10.5455/ajvs.186038



APA (American Psychological Association) Style

Jehan A. Gafer, Ghada H. Abd El-Rahman, Rawash Z. M. (2015) Association of Hsp70 Gene Polymorphism And Bull Semen Quality In Winter And Summer Seasons. Alexandria Journal of Veterinary Sciences, 46 (1), 1-14. doi:10.5455/ajvs.186038





Most Viewed Articles
Most Accessed Articles

  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Most Downloaded
    Top Downloaded Articles

  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Effect of Excess Fluoride on Reproductive Potentials in Farm Animals (Ovine)
    Ghada H. Abdel-Rahman, Hanaa A. El-Hallawany, Dohreig RA.
    AJVS. 2018; 57(2): 41-57
    » Abstract » doi: 10.5455/ajvs.296364

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract » doi: 10.5455/ajvs.47290

  • Most Cited Articles
    Most Cited Articles