E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate

Sarah E. AlBeltagi, Helmy A. Torky, Mahmoud E. Seddeek.

Abstract
Seventeen avian infectious bronchitis virus (IBV) isolates were isolated from broiler chickens showing respiratory and renal lesions. The isolated strains were characterized by real time reverse transcriptase polymerase chain reaction used for N gene, and then RT-PCR and sequence analysis of the hypervariable region 3 of the S1 spike glycoprotein gene of six isolates. Six isolates showed 87.15% to 89.71 % and 87.27% to 90.82% amino acid sequence identity and 87.61% to 89.19 % and 87.91% to 89.72% nucleotide sequence identity to the Egyptian variant 1 and the IS/885 strains, respectively. The six isolates formed a distinct phylogenetic group with the Ck/Eg/BSU-2/2011 and Ck/Eg/ BSU-3/2011 (Var 2). Amino acid and nucleotide identities between the six Egyptian isolates and variant 2 (Ck/Eg/BSU-2/2011 and Ck/Eg/BSU-3/2011) ranged from 97.27% to 100% and 97.88% to 99.38%, respectively. The results indicate that the six isolates IBV/CK/Beh/101/013/S1,IBV/CK/Beh/204/013/S1,IBV/CK/Beh/105/013/S1,IBV/CK/Beh/1011/013/S1,IBV/CK/Beh/1017/013/S1,IBV/CK/Beh/2020/013/S1 can be considered a variant 2 as Ck/Eg/BSU-2/2011 and Ck/Eg/BSU-3/2011. This study demonstrates a constant evolution of IBV in Egypt that necessitates continuous monitoring to control the spread of infections, and the development and use of vaccines based on indigenous viruses.

Key words: Antigenic variations, Infectious Bronchitis virus, RT-PCR, Sequencing.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Sarah E. AlBeltagi
Articles by Helmy A. Torky
Articles by Mahmoud E. Seddeek
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Sarah E. AlBeltagi, Helmy A. Torky, Mahmoud E. Seddeek. Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate. AJVS. 2014; 40(1): 44-51. doi:10.5455/ajvs.46652


Web Style

Sarah E. AlBeltagi, Helmy A. Torky, Mahmoud E. Seddeek. Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate. https://www.alexjvs.com/?mno=46652 [Access: December 05, 2023]. doi:10.5455/ajvs.46652


AMA (American Medical Association) Style

Sarah E. AlBeltagi, Helmy A. Torky, Mahmoud E. Seddeek. Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate. AJVS. 2014; 40(1): 44-51. doi:10.5455/ajvs.46652



Vancouver/ICMJE Style

Sarah E. AlBeltagi, Helmy A. Torky, Mahmoud E. Seddeek. Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate. AJVS. (2014), [cited December 05, 2023]; 40(1): 44-51. doi:10.5455/ajvs.46652



Harvard Style

Sarah E. AlBeltagi, Helmy A. Torky, Mahmoud E. Seddeek (2014) Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate. AJVS, 40 (1), 44-51. doi:10.5455/ajvs.46652



Turabian Style

Sarah E. AlBeltagi, Helmy A. Torky, Mahmoud E. Seddeek. 2014. Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate. Alexandria Journal of Veterinary Sciences, 40 (1), 44-51. doi:10.5455/ajvs.46652



Chicago Style

Sarah E. AlBeltagi, Helmy A. Torky, Mahmoud E. Seddeek. "Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate." Alexandria Journal of Veterinary Sciences 40 (2014), 44-51. doi:10.5455/ajvs.46652



MLA (The Modern Language Association) Style

Sarah E. AlBeltagi, Helmy A. Torky, Mahmoud E. Seddeek. "Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate." Alexandria Journal of Veterinary Sciences 40.1 (2014), 44-51. Print. doi:10.5455/ajvs.46652



APA (American Psychological Association) Style

Sarah E. AlBeltagi, Helmy A. Torky, Mahmoud E. Seddeek (2014) Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate. Alexandria Journal of Veterinary Sciences, 40 (1), 44-51. doi:10.5455/ajvs.46652





Most Viewed Articles
Most Accessed Articles

  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Most Downloaded
    Top Downloaded Articles

  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Effect of Excess Fluoride on Reproductive Potentials in Farm Animals (Ovine)
    Ghada H. Abdel-Rahman, Hanaa A. El-Hallawany, Dohreig RA.
    AJVS. 2018; 57(2): 41-57
    » Abstract » doi: 10.5455/ajvs.296364

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract » doi: 10.5455/ajvs.47290

  • Most Cited Articles
    Most Cited Articles