Phenotypic and Genotypic Characterization of Pseudomonas Aerogenosa Isolated from Bovine Mastitis

Amel E. Ghazy; Mohamed I. Alkatsha; Samy A. Khaliel; Mohamed E. Noseir

doi:10.5455/ajvs.1619

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Original Article
Online Published: 13 Feb 2015

AJVS. 2015; 44(1): 80-85

doi: 10.5455/ajvs.1619

Phenotypic and Genotypic Characterization of Pseudomonas Aerogenosa Isolated from Bovine Mastitis

Amel E. Ghazy, Mohamed I. Alkatsha, Samy A. Khaliel, Mohamed E. Noseir.

Abstract
Pseudomonas aeruginosa is naturally resistant to wide spectrum of antibiotics in current use and infections by this bacterium have been found very refractory to chemotherapy so in this study we attempt to study the incidence and resistance pattern of pseudomonas aeruginosa in cattle. The present study was carried on 150 samples randomly collected from different farms at El-Behera and Alexandria goveronates from mastitic cows.
The samples were directly transferred to laboratory where they were prepared for bacteriological examination for isolation of pseudomonas aeruginosa , Determination of anti-bacterial sensitivity test and isolation of plasmids. Result of this study showed that 15 out of 150 samples (10%) were positive for pseudomonas aeruginosa

Result of this study showed that 15 isolates of pseudomonas aeruginosa were tested against 9 antibacterial agents. The organism was sensitive to ciprofloxacin, vancomycin.Fifteen pseudomonas aeruginosa isolates were positive for analysis by using polyacrylamide gel electrophoresis.The carried plasmids with molecular weight of 18Kbp.Ih could be concluded that Pseudomanas aerogenosa was resistant to the most antibiotics and carrying plasmid DNA. Moreover, antibiotic is important for the persistence of plasmid DNA.
Keywords : Phenotypic-genotypic-plasmids-pseudomonas

Key words: Phenotypic-genotypic-plasmids-pseudomonas

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How to Cite this Article

Pubmed Style

Ghazy AE, Alkatsha MI, Khaliel SA, Noseir ME. Phenotypic and Genotypic Characterization of Pseudomonas Aerogenosa Isolated from Bovine Mastitis. AJVS. 2015; 44(1): 80-85. doi:10.5455/ajvs.1619

Web Style

Ghazy AE, Alkatsha MI, Khaliel SA, Noseir ME. Phenotypic and Genotypic Characterization of Pseudomonas Aerogenosa Isolated from Bovine Mastitis. https://www.alexjvs.com/?mno=1619 [Access: May 04, 2025]. doi:10.5455/ajvs.1619

AMA (American Medical Association) Style

Ghazy AE, Alkatsha MI, Khaliel SA, Noseir ME. Phenotypic and Genotypic Characterization of Pseudomonas Aerogenosa Isolated from Bovine Mastitis. AJVS. 2015; 44(1): 80-85. doi:10.5455/ajvs.1619

Vancouver/ICMJE Style

Ghazy AE, Alkatsha MI, Khaliel SA, Noseir ME. Phenotypic and Genotypic Characterization of Pseudomonas Aerogenosa Isolated from Bovine Mastitis. AJVS. (2015), [cited May 04, 2025]; 44(1): 80-85. doi:10.5455/ajvs.1619

Harvard Style

Ghazy, A. E., Alkatsha, . M. I., Khaliel, . S. A. & Noseir, . M. E. (2015) Phenotypic and Genotypic Characterization of Pseudomonas Aerogenosa Isolated from Bovine Mastitis. AJVS, 44 (1), 80-85. doi:10.5455/ajvs.1619

Turabian Style

Ghazy, Amel E., Mohamed I. Alkatsha, Samy A. Khaliel, and Mohamed E. Noseir. 2015. Phenotypic and Genotypic Characterization of Pseudomonas Aerogenosa Isolated from Bovine Mastitis. Alexandria Journal of Veterinary Sciences, 44 (1), 80-85. doi:10.5455/ajvs.1619

Chicago Style

Ghazy, Amel E., Mohamed I. Alkatsha, Samy A. Khaliel, and Mohamed E. Noseir. "Phenotypic and Genotypic Characterization of Pseudomonas Aerogenosa Isolated from Bovine Mastitis." Alexandria Journal of Veterinary Sciences 44 (2015), 80-85. doi:10.5455/ajvs.1619

MLA (The Modern Language Association) Style

APA (American Psychological Association) Style

Ghazy, A. E., Alkatsha, . M. I., Khaliel, . S. A. & Noseir, . M. E. (2015) Phenotypic and Genotypic Characterization of Pseudomonas Aerogenosa Isolated from Bovine Mastitis. Alexandria Journal of Veterinary Sciences, 44 (1), 80-85. doi:10.5455/ajvs.1619

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