Polymerase Chain Reaction (PCR): An Alternative Rapid Method for Detection of Some Microbial Contamination of Meat Products

Reda Tarabees; Zakaria H. Hassanin; EL Bagoury; A.M.

doi:10.5455/ajvs.177446

2025, Vol: 87

87Current Volume Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

« Previous Article

Original Article
Online Published: 15 Apr 2015

AJVS. 2015; 45(1): 91-98

doi: 10.5455/ajvs.177446

Polymerase Chain Reaction (PCR): An Alternative Rapid Method for Detection of Some Microbial Contamination of Meat Products

Reda Tarabees; Zakaria H. Hassanin; El Bagoury, A.m..

Abstract
The use of advanced molecular techniques such as PCR has been introduced as a new fast and reliable method for detection of microbial contamination of food stuff. In this study, a total 160 samples of meat products (40 luncheon, 40 beef burger, 40 minced meat, and 40 fresh sausage) were examined for the presence of E. coli and pathogenic S. aureus using traditional methods of isolation and identification and PCR. The data obtained from this study showed that E. coli was isolated from 9 (22.5%), 13 (32.5%), 19 (47.5%), and 24 (60%) by traditional method comparing with 8 (20%), 13 (32.5%), 17 (42.5%), 23 (57.5%) out of 40 samples of luncheon, beef burger, minced meat, and fresh sausage samples respectively. While, S. aureus was isolated from 17 (42.5%), 11 (27.5%), 28 (70%), and 18 (45%) using traditional methods of isolation in comparison with 13 (32.5%), 8 (20%) 23 (57.5%), and 14 (35%) using PCR method out of 40 samples of luncheon, beef burger, minced meat, and fresh sausage samples correspondingly, with a sensitivity rate ranged from 87 to 96% in case of E. coli and 72 to 82% in case of S. aureus. Collectively, the data presented have revealed that, PCR is considered a fast, reliable and sensitive tool for detection of microbial contamination of food stuff.

Key words: PCR, Meat products, E. coli, S. aureus

	ARTICLE TOOLS
	Abstract
	PDF Fulltext
	How to cite this article
	Citation Tools
	Related Records
	Articles by Reda Tarabees; Zakaria H. Hassanin; EL Bagoury Articles by A.M.
	on Google
	on Google Scholar

How to Cite this Article

Pubmed Style

Tarabees R, Hassanin ZH, Bagoury E, A.m. . Polymerase Chain Reaction (PCR): An Alternative Rapid Method for Detection of Some Microbial Contamination of Meat Products. AJVS. 2015; 45(1): 91-98. doi:10.5455/ajvs.177446

Web Style

Tarabees R, Hassanin ZH, Bagoury E, A.m. . Polymerase Chain Reaction (PCR): An Alternative Rapid Method for Detection of Some Microbial Contamination of Meat Products. https://www.alexjvs.com/?mno=177446 [Access: May 03, 2025]. doi:10.5455/ajvs.177446

AMA (American Medical Association) Style

Tarabees R, Hassanin ZH, Bagoury E, A.m. . Polymerase Chain Reaction (PCR): An Alternative Rapid Method for Detection of Some Microbial Contamination of Meat Products. AJVS. 2015; 45(1): 91-98. doi:10.5455/ajvs.177446

Vancouver/ICMJE Style

Tarabees R, Hassanin ZH, Bagoury E, A.m. . Polymerase Chain Reaction (PCR): An Alternative Rapid Method for Detection of Some Microbial Contamination of Meat Products. AJVS. (2015), [cited May 03, 2025]; 45(1): 91-98. doi:10.5455/ajvs.177446

Harvard Style

Tarabees, R., Hassanin, . Z. H., Bagoury, . E. & A.m., . (2015) Polymerase Chain Reaction (PCR): An Alternative Rapid Method for Detection of Some Microbial Contamination of Meat Products. AJVS, 45 (1), 91-98. doi:10.5455/ajvs.177446

Turabian Style

Tarabees, Reda, Zakaria H. Hassanin, El Bagoury, and A.m.. 2015. Polymerase Chain Reaction (PCR): An Alternative Rapid Method for Detection of Some Microbial Contamination of Meat Products. Alexandria Journal of Veterinary Sciences, 45 (1), 91-98. doi:10.5455/ajvs.177446

Chicago Style

Tarabees, Reda, Zakaria H. Hassanin, El Bagoury, and A.m.. "Polymerase Chain Reaction (PCR): An Alternative Rapid Method for Detection of Some Microbial Contamination of Meat Products." Alexandria Journal of Veterinary Sciences 45 (2015), 91-98. doi:10.5455/ajvs.177446

MLA (The Modern Language Association) Style

APA (American Psychological Association) Style

About Alexandria Journal of Veterinary Sciences

Contact Information

How to cite this article