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Original Article
Online Published: 15 Apr 2015
 


Polymerase Chain Reaction (PCR): An Alternative Rapid Method for Detection of Some Microbial Contamination of Meat Products

Reda Tarabees; Zakaria H. Hassanin; EL Bagoury, A.M..


Abstract
The use of advanced molecular techniques such as PCR has been introduced as a new fast and reliable method for detection of microbial contamination of food stuff. In this study, a total 160 samples of meat products (40 luncheon, 40 beef burger, 40 minced meat, and 40 fresh sausage) were examined for the presence of E. coli and pathogenic S. aureus using traditional methods of isolation and identification and PCR. The data obtained from this study showed that E. coli was isolated from 9 (22.5%), 13 (32.5%), 19 (47.5%), and 24 (60%) by traditional method comparing with 8 (20%), 13 (32.5%), 17 (42.5%), 23 (57.5%) out of 40 samples of luncheon, beef burger, minced meat, and fresh sausage samples respectively. While, S. aureus was isolated from 17 (42.5%), 11 (27.5%), 28 (70%), and 18 (45%) using traditional methods of isolation in comparison with 13 (32.5%), 8 (20%) 23 (57.5%), and 14 (35%) using PCR method out of 40 samples of luncheon, beef burger, minced meat, and fresh sausage samples correspondingly, with a sensitivity rate ranged from 87 to 96% in case of E. coli and 72 to 82% in case of S. aureus. Collectively, the data presented have revealed that, PCR is considered a fast, reliable and sensitive tool for detection of microbial contamination of food stuff.

Key words: PCR, Meat products, E. coli, S. aureus


 
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How to Cite this Article
Pubmed Style

Tarabees R, Hassanin ZH, A.M. EB. Polymerase Chain Reaction (PCR): An Alternative Rapid Method for Detection of Some Microbial Contamination of Meat Products. AJVS. 2015; 45(1): 91-98. doi:10.5455/ajvs.177446

Web Style

Tarabees R, Hassanin ZH, A.M. EB. Polymerase Chain Reaction (PCR): An Alternative Rapid Method for Detection of Some Microbial Contamination of Meat Products. https://www.alexjvs.com/?mno=177446 [Access: December 02, 2024]. doi:10.5455/ajvs.177446

AMA (American Medical Association) Style

Tarabees R, Hassanin ZH, A.M. EB. Polymerase Chain Reaction (PCR): An Alternative Rapid Method for Detection of Some Microbial Contamination of Meat Products. AJVS. 2015; 45(1): 91-98. doi:10.5455/ajvs.177446


Vancouver/ICMJE Style

Tarabees R, Hassanin ZH, A.M. EB. Polymerase Chain Reaction (PCR): An Alternative Rapid Method for Detection of Some Microbial Contamination of Meat Products. AJVS. (2015), [cited December 02, 2024]; 45(1): 91-98. doi:10.5455/ajvs.177446


Harvard Style

Tarabees, R., Hassanin, . Z. H. & A.M., . E. B. (2015) Polymerase Chain Reaction (PCR): An Alternative Rapid Method for Detection of Some Microbial Contamination of Meat Products. AJVS, 45 (1), 91-98. doi:10.5455/ajvs.177446


Turabian Style

Tarabees, Reda, Zakaria H. Hassanin, and EL Bagoury, A.M.. 2015. Polymerase Chain Reaction (PCR): An Alternative Rapid Method for Detection of Some Microbial Contamination of Meat Products. Alexandria Journal of Veterinary Sciences, 45 (1), 91-98. doi:10.5455/ajvs.177446


Chicago Style

Tarabees, Reda, Zakaria H. Hassanin, and EL Bagoury, A.M.. "Polymerase Chain Reaction (PCR): An Alternative Rapid Method for Detection of Some Microbial Contamination of Meat Products." Alexandria Journal of Veterinary Sciences 45 (2015), 91-98. doi:10.5455/ajvs.177446


MLA (The Modern Language Association) Style

Tarabees, Reda, Zakaria H. Hassanin, and EL Bagoury, A.M.. "Polymerase Chain Reaction (PCR): An Alternative Rapid Method for Detection of Some Microbial Contamination of Meat Products." Alexandria Journal of Veterinary Sciences 45.1 (2015), 91-98. Print. doi:10.5455/ajvs.177446


APA (American Psychological Association) Style

Tarabees, R., Hassanin, . Z. H. & A.M., . E. B. (2015) Polymerase Chain Reaction (PCR): An Alternative Rapid Method for Detection of Some Microbial Contamination of Meat Products. Alexandria Journal of Veterinary Sciences, 45 (1), 91-98. doi:10.5455/ajvs.177446




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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