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Original Article
Online Published: 15 Apr 2015
 


Some Studies on E-Coli Mastitis in Cattle and Buffaloes

Abdel kerim A.Mahmoud, Adel M. Khadr, Tharwat M. Elshemy, Hassan A. Hamoda, Mohamed I. Ismail.


Abstract
The aim of this study is to identify the role of E.coli in bovine mastitis, its virulence factors and antimicrobial sensitivity. 360 lactating cows and buffaloes were examined clinically and by CMT, 210 were suffering from clinical mastitis with percentage of 62.7% and 39 (20.36%) E.coli isolates were detected in clinical cases of mastitis, 90 cases were suffering from subclinical mastitis and 6 (6.8%) E.coli isolates were detected in them. The percentage of E.coli in cows was 31 (21.142%) and in buffaloes 8 (18.33%) isolates. Serotyping of E.coli revealed that O55 (30%), O111 (15%), O124 (15%), O119 (10%), O114 (10%), O26 (5%), O157 (10%) and O44 (5%). That’s showing that O55, O111 and O124 the most serotypes causing mastitis. PCR identification of TraT gene revealed 5 cases were positive and give positive reaction at 307 bp. and for eaeA gene revealed 6 cases and give positive reaction at 384 bp. The antimicrobial sensitivity indicated that the most effective antibiotics were lincospectine (56.6%), danofloxacin (56.6%), enrofloxacin (40%) and ceftifure (40%), while the lowest effective antibiotics were oxytetracycline and ampicillin.

Key words: E.coli, Cattle, Buffaloes, Serological identification, PCR


 
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How to Cite this Article
Pubmed Style

Abdel kerim A.Mahmoud, Adel M. Khadr, Tharwat M. Elshemy, Hassan A. Hamoda, Mohamed I. Ismail. Some Studies on E-Coli Mastitis in Cattle and Buffaloes. AJVS. 2015; 45(1): 105-112. doi:10.5455/ajvs.178113

Web Style

Abdel kerim A.Mahmoud, Adel M. Khadr, Tharwat M. Elshemy, Hassan A. Hamoda, Mohamed I. Ismail. Some Studies on E-Coli Mastitis in Cattle and Buffaloes. https://www.alexjvs.com/?mno=178113 [Access: December 02, 2024]. doi:10.5455/ajvs.178113

AMA (American Medical Association) Style

Abdel kerim A.Mahmoud, Adel M. Khadr, Tharwat M. Elshemy, Hassan A. Hamoda, Mohamed I. Ismail. Some Studies on E-Coli Mastitis in Cattle and Buffaloes. AJVS. 2015; 45(1): 105-112. doi:10.5455/ajvs.178113


Vancouver/ICMJE Style

Abdel kerim A.Mahmoud, Adel M. Khadr, Tharwat M. Elshemy, Hassan A. Hamoda, Mohamed I. Ismail. Some Studies on E-Coli Mastitis in Cattle and Buffaloes. AJVS. (2015), [cited December 02, 2024]; 45(1): 105-112. doi:10.5455/ajvs.178113


Harvard Style

Abdel kerim A.Mahmoud, Adel M. Khadr, Tharwat M. Elshemy, Hassan A. Hamoda, Mohamed I. Ismail (2015) Some Studies on E-Coli Mastitis in Cattle and Buffaloes. AJVS, 45 (1), 105-112. doi:10.5455/ajvs.178113


Turabian Style

Abdel kerim A.Mahmoud, Adel M. Khadr, Tharwat M. Elshemy, Hassan A. Hamoda, Mohamed I. Ismail. 2015. Some Studies on E-Coli Mastitis in Cattle and Buffaloes. Alexandria Journal of Veterinary Sciences, 45 (1), 105-112. doi:10.5455/ajvs.178113


Chicago Style

Abdel kerim A.Mahmoud, Adel M. Khadr, Tharwat M. Elshemy, Hassan A. Hamoda, Mohamed I. Ismail. "Some Studies on E-Coli Mastitis in Cattle and Buffaloes." Alexandria Journal of Veterinary Sciences 45 (2015), 105-112. doi:10.5455/ajvs.178113


MLA (The Modern Language Association) Style

Abdel kerim A.Mahmoud, Adel M. Khadr, Tharwat M. Elshemy, Hassan A. Hamoda, Mohamed I. Ismail. "Some Studies on E-Coli Mastitis in Cattle and Buffaloes." Alexandria Journal of Veterinary Sciences 45.1 (2015), 105-112. Print. doi:10.5455/ajvs.178113


APA (American Psychological Association) Style

Abdel kerim A.Mahmoud, Adel M. Khadr, Tharwat M. Elshemy, Hassan A. Hamoda, Mohamed I. Ismail (2015) Some Studies on E-Coli Mastitis in Cattle and Buffaloes. Alexandria Journal of Veterinary Sciences, 45 (1), 105-112. doi:10.5455/ajvs.178113




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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    Egypt.

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