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Original Article
Online Published: 04 Aug 2015
 


Detection of Molecular Characterization of Salmonella enterica isolated from Chicken Meat and Its Products by Using Multiplex PCR

Heba S. Amin and Abd El-Rahman A. Abd El-Rahman.


Abstract
The present study was conducted for identification of molecular characterization of Salmonella enterica isolated from 200 samples of fresh chicken meat and 100 samples of ready to eat chicken meat. Samples were assessed by performing the pre-enrichment and enrichment culture, biochemically characterized by the analytical profiling index (API 20E system). The primers were selected for invA, stn, avrA, sefA, fliC and stm 4495 genes, specific for the detection of Salmonella enteritidis, Salmonella typhimurium and Salmonella kentucky. Standard bacteriological examination revealed that 7 S. enterica isolated from 200 chicken meats with a percentage of (3.5%), including 5 S. typhimurium with a percentage of (2.5%) and 1 for each of S. enteritidis and S. kentucky with a percentage of (0.5 %), while could not isolate S. enterica from ready to eat chicken meat. The results of multiplex PCR for the three isolated serotypes of Salmonella enterica (1 for each S. typhimurium, S. Kentucky and S. enteritidis) could detect the universal gene (invA) and virulence genes (avrA and stn) in all examined serotypes, while (fliC) gene was detected in both S. typhimurium and S. kentucky only while not detected in S. enteritidis, (stm 4495) gene was detected only in S. typhimurium but was not detected in S. kentucky and S. enteritidis. (sefA) gene was detected only in S. enteritidis but was not detected in S. typhimurium and S. kentucky .

Key words: S. typhimurium - S. enteritidis - S. Kentucky - chicken meat - Multiplex-PCR.


 
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Pubmed Style

Heba S. Amin and Abd El-Rahman A. Abd El-Rahman. Detection of Molecular Characterization of Salmonella enterica isolated from Chicken Meat and Its Products by Using Multiplex PCR. AJVS. 2015; 46(1): 155-160. doi:10.5455/ajvs.196302

Web Style

Heba S. Amin and Abd El-Rahman A. Abd El-Rahman. Detection of Molecular Characterization of Salmonella enterica isolated from Chicken Meat and Its Products by Using Multiplex PCR. https://www.alexjvs.com/?mno=196302 [Access: December 02, 2024]. doi:10.5455/ajvs.196302

AMA (American Medical Association) Style

Heba S. Amin and Abd El-Rahman A. Abd El-Rahman. Detection of Molecular Characterization of Salmonella enterica isolated from Chicken Meat and Its Products by Using Multiplex PCR. AJVS. 2015; 46(1): 155-160. doi:10.5455/ajvs.196302


Vancouver/ICMJE Style

Heba S. Amin and Abd El-Rahman A. Abd El-Rahman. Detection of Molecular Characterization of Salmonella enterica isolated from Chicken Meat and Its Products by Using Multiplex PCR. AJVS. (2015), [cited December 02, 2024]; 46(1): 155-160. doi:10.5455/ajvs.196302


Harvard Style

Heba S. Amin and Abd El-Rahman A. Abd El-Rahman (2015) Detection of Molecular Characterization of Salmonella enterica isolated from Chicken Meat and Its Products by Using Multiplex PCR. AJVS, 46 (1), 155-160. doi:10.5455/ajvs.196302


Turabian Style

Heba S. Amin and Abd El-Rahman A. Abd El-Rahman. 2015. Detection of Molecular Characterization of Salmonella enterica isolated from Chicken Meat and Its Products by Using Multiplex PCR. Alexandria Journal of Veterinary Sciences, 46 (1), 155-160. doi:10.5455/ajvs.196302


Chicago Style

Heba S. Amin and Abd El-Rahman A. Abd El-Rahman. "Detection of Molecular Characterization of Salmonella enterica isolated from Chicken Meat and Its Products by Using Multiplex PCR." Alexandria Journal of Veterinary Sciences 46 (2015), 155-160. doi:10.5455/ajvs.196302


MLA (The Modern Language Association) Style

Heba S. Amin and Abd El-Rahman A. Abd El-Rahman. "Detection of Molecular Characterization of Salmonella enterica isolated from Chicken Meat and Its Products by Using Multiplex PCR." Alexandria Journal of Veterinary Sciences 46.1 (2015), 155-160. Print. doi:10.5455/ajvs.196302


APA (American Psychological Association) Style

Heba S. Amin and Abd El-Rahman A. Abd El-Rahman (2015) Detection of Molecular Characterization of Salmonella enterica isolated from Chicken Meat and Its Products by Using Multiplex PCR. Alexandria Journal of Veterinary Sciences, 46 (1), 155-160. doi:10.5455/ajvs.196302




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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