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Original Article
Online Published: 19 Feb 2016
 


Efficacy and Safety of Different Antimycotic Formulas Used in Sheep Frozen Semen Extenders

Fadwa M. Othman, Diya A. El-badry, Mahmoud I. Mohamed, Mona H. Shaker, Hossam R. Kotb And Helmy A.torky.


Abstract
This study aimed to select a potent and safe antimycotic as additive to cryodiluents of sheep frozen semen. Semen samples were collected from 6 Barki rams and processed for cryopreservation after adding different concentrations of griseovulvin, clotrimazole, nystatin, fluconazole or amphotricin-B to semen diluent. Sensitivity of Candida albicans and Cryptococcus neoformans to antimycotic drugs were studied. Results showed that fluconazole and nystatin (at dose not higher than 50 mg/L) had no deleterious effect on sheep semen freezability. Higher doses of amphotricin-B and griseofulvin resulted in drastic deterioration of frozen semen quality. Candida albicans and Cryptococcus neoformans were sensitive to fluconazole and amphotricin-B and resistant to lower concentrations of griseovulvin and clotrimazole. Conclusively, addition of fluconazole to sheep semen diluent has a benefit of improving the quality of yeast contaminated sperm with no bad effect on the frozen-thawed semen quality.

Key words: anti-mycotic, griseovulvin, clotrimazole, nystatin, fluconazole, amphotricin-B, Candida, Cryptococcus, frozen semen, sheep


 
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How to Cite this Article
Pubmed Style

Othman FM, El-badry DA, Mohamed MI, Shaker MH, . Efficacy and Safety of Different Antimycotic Formulas Used in Sheep Frozen Semen Extenders. AJVS. 2016; 48(2): 77-82. doi:10.5455/ajvs.202266

Web Style

Othman FM, El-badry DA, Mohamed MI, Shaker MH, . Efficacy and Safety of Different Antimycotic Formulas Used in Sheep Frozen Semen Extenders. https://www.alexjvs.com/?mno=202266 [Access: May 04, 2025]. doi:10.5455/ajvs.202266

AMA (American Medical Association) Style

Othman FM, El-badry DA, Mohamed MI, Shaker MH, . Efficacy and Safety of Different Antimycotic Formulas Used in Sheep Frozen Semen Extenders. AJVS. 2016; 48(2): 77-82. doi:10.5455/ajvs.202266


Vancouver/ICMJE Style

Othman FM, El-badry DA, Mohamed MI, Shaker MH, . Efficacy and Safety of Different Antimycotic Formulas Used in Sheep Frozen Semen Extenders. AJVS. (2016), [cited May 04, 2025]; 48(2): 77-82. doi:10.5455/ajvs.202266


Harvard Style

Othman, F. M., El-badry, . D. A., Mohamed, . M. I., Shaker, . M. H. & (2016) Efficacy and Safety of Different Antimycotic Formulas Used in Sheep Frozen Semen Extenders. AJVS, 48 (2), 77-82. doi:10.5455/ajvs.202266


Turabian Style

Othman, Fadwa M., Diya A. El-badry, Mahmoud I. Mohamed, Mona H. Shaker, and Hossam R. Kotb And Helmy A.torky. 2016. Efficacy and Safety of Different Antimycotic Formulas Used in Sheep Frozen Semen Extenders. Alexandria Journal of Veterinary Sciences, 48 (2), 77-82. doi:10.5455/ajvs.202266


Chicago Style

Othman, Fadwa M., Diya A. El-badry, Mahmoud I. Mohamed, Mona H. Shaker, and Hossam R. Kotb And Helmy A.torky. "Efficacy and Safety of Different Antimycotic Formulas Used in Sheep Frozen Semen Extenders." Alexandria Journal of Veterinary Sciences 48 (2016), 77-82. doi:10.5455/ajvs.202266


MLA (The Modern Language Association) Style

Othman, Fadwa M., Diya A. El-badry, Mahmoud I. Mohamed, Mona H. Shaker, and Hossam R. Kotb And Helmy A.torky. "Efficacy and Safety of Different Antimycotic Formulas Used in Sheep Frozen Semen Extenders." Alexandria Journal of Veterinary Sciences 48.2 (2016), 77-82. Print. doi:10.5455/ajvs.202266


APA (American Psychological Association) Style

Othman, F. M., El-badry, . D. A., Mohamed, . M. I., Shaker, . M. H. & (2016) Efficacy and Safety of Different Antimycotic Formulas Used in Sheep Frozen Semen Extenders. Alexandria Journal of Veterinary Sciences, 48 (2), 77-82. doi:10.5455/ajvs.202266




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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    Egypt.

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