Efficacy and safety of different antimycotic formulas used in sheep frozen semen extenders

Fadwa M. Othman; Mahmud I. Mohamed; Diyaud Din A. El-Badry; Mona H. Shaker; Mohamed hosam R. Kotb; Helmy A. Turky

2025, Vol: 86

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Original Article

AJVS. 0; 0(0):

Efficacy and safety of different antimycotic formulas used in sheep frozen semen extenders

Fadwa M. Othman, Mahmud I. Mohamed, Diyaud Din A. El-badry, Mona H. Shaker, Mohamed Hosam R. Kotb, Helmy A. Turky.

Abstract
This study aimed to select a potent and safe antimycotic as additive to cryodiluents of sheep frozen semen. Semen samples were collected from 6 Barki rams and processed for cryopreservation after adding different concentrations of griseovulvin, clotrimazole, nystatin, fluconazole and amphotricin-B to semen diluent. Sensitivity of Candida albicans and Cryptococcus neoformans to antimycotic drugs were studied. Results showed that fluconazole and nystatin (at dose not higher than 50 mg/L) had no deleterious effect on sheep semen freezability. Higher doses of amphotricin-B and griseofulvin resulted in drastic deterioration of frozen semen quality. Candida albicans and Cryptococcus neoformans were sensitive to fluconazole and amphotricin-B and resistant to lower concentrations of griseovulvin and clotrimazole. Conclusively, addition of fluconazole to sheep semen diluent has a benefit of improving the quality of yeast contaminated sperm with no bad effect on the frozen-thawed semen quality.

Key words: : anti-mycotic, amphotricin-B, Candida, Cryptococcus, frozen semen, sheep

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Pubmed Style

Othman FM, Mohamed MI, El-badry DDA, Shaker MH, Kotb MHR, Turky HA. Efficacy and safety of different antimycotic formulas used in sheep frozen semen extenders. AJVS. 0; 0(0): -.

Web Style

Othman FM, Mohamed MI, El-badry DDA, Shaker MH, Kotb MHR, Turky HA. Efficacy and safety of different antimycotic formulas used in sheep frozen semen extenders. https://www.alexjvs.com/?mno=202267 [Access: May 03, 2025].

AMA (American Medical Association) Style

Othman FM, Mohamed MI, El-badry DDA, Shaker MH, Kotb MHR, Turky HA. Efficacy and safety of different antimycotic formulas used in sheep frozen semen extenders. AJVS. 0; 0(0): -.

Vancouver/ICMJE Style

Othman FM, Mohamed MI, El-badry DDA, Shaker MH, Kotb MHR, Turky HA. Efficacy and safety of different antimycotic formulas used in sheep frozen semen extenders. AJVS. (0), [cited May 03, 2025]; 0(0): -.

Harvard Style

Othman, F. M., Mohamed, . M. I., El-badry, . D. D. A., Shaker, . M. H., Kotb, . M. H. R. & Turky, . H. A. (0) Efficacy and safety of different antimycotic formulas used in sheep frozen semen extenders. AJVS, 0 (0), -.

Turabian Style

Othman, Fadwa M., Mahmud I. Mohamed, Diyaud Din A. El-badry, Mona H. Shaker, Mohamed Hosam R. Kotb, and Helmy A. Turky. 0. Efficacy and safety of different antimycotic formulas used in sheep frozen semen extenders. Alexandria Journal of Veterinary Sciences, 0 (0), -.

Chicago Style

Othman, Fadwa M., Mahmud I. Mohamed, Diyaud Din A. El-badry, Mona H. Shaker, Mohamed Hosam R. Kotb, and Helmy A. Turky. "Efficacy and safety of different antimycotic formulas used in sheep frozen semen extenders." Alexandria Journal of Veterinary Sciences 0 (0), -.

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