E-ISSN 2536-9520 | ISSN 1110-2047
 
2024, Vol: 83
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Original Article 


Genotypic molecular detection of certain genes encoding virulence determinates and antibiotic resistance in Staphylococcus aureus isolates from mastitic cows

Ahmed Lotfy.


Abstract
A total of 10Staphylococcusaureusisolates were recoveredfrom 117 milk samples whichwere obtained from 93 dairy cows indifferent farms suffered fromclinical mastitis, theseisolateswere phenotypically characterized and identified based on colony morphology andbiochemical tests. Themolecular detection of S. aureus specific sequence gene(Sa),genes encoding clumpingfactor A(clfA), (spa) gene encoding S.aureus protein A and the class 1 integron using PolymeraseChain Reaction were carriedout. All isolateswere negative for the presence of class 1 integronbut yielded an amplicon size of about107 bp in case of S. aureus specific sequence gene (Sa),an amplicon size of about 638bp in case ofclfA gene. Amplification of thespa gene encoding S. aureus protein Awas detected in 9 strains out of 10 (90%)and yielded an amplicon size of about 226bp.

Key words: Molecular detection, Staphylococcus aureus isolates, mastitic cows


 
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Pubmed Style

Ahmed Lotfy. Genotypic molecular detection of certain genes encoding virulence determinates and antibiotic resistance in Staphylococcus aureus isolates from mastitic cows. AJVS. 0; 0(0): -.

Web Style

Ahmed Lotfy. Genotypic molecular detection of certain genes encoding virulence determinates and antibiotic resistance in Staphylococcus aureus isolates from mastitic cows. https://www.alexjvs.com/?mno=216450 [Access: September 12, 2024].

AMA (American Medical Association) Style

Ahmed Lotfy. Genotypic molecular detection of certain genes encoding virulence determinates and antibiotic resistance in Staphylococcus aureus isolates from mastitic cows. AJVS. 0; 0(0): -.


Vancouver/ICMJE Style

Ahmed Lotfy. Genotypic molecular detection of certain genes encoding virulence determinates and antibiotic resistance in Staphylococcus aureus isolates from mastitic cows. AJVS. (0), [cited September 12, 2024]; 0(0): -.


Harvard Style

Ahmed Lotfy (0) Genotypic molecular detection of certain genes encoding virulence determinates and antibiotic resistance in Staphylococcus aureus isolates from mastitic cows. AJVS, 0 (0), -.


Turabian Style

Ahmed Lotfy. 0. Genotypic molecular detection of certain genes encoding virulence determinates and antibiotic resistance in Staphylococcus aureus isolates from mastitic cows. Alexandria Journal of Veterinary Sciences, 0 (0), -.


Chicago Style

Ahmed Lotfy. "Genotypic molecular detection of certain genes encoding virulence determinates and antibiotic resistance in Staphylococcus aureus isolates from mastitic cows." Alexandria Journal of Veterinary Sciences 0 (0), -.


MLA (The Modern Language Association) Style

Ahmed Lotfy. "Genotypic molecular detection of certain genes encoding virulence determinates and antibiotic resistance in Staphylococcus aureus isolates from mastitic cows." Alexandria Journal of Veterinary Sciences 0.0 (0), -. Print.


APA (American Psychological Association) Style

Ahmed Lotfy (0) Genotypic molecular detection of certain genes encoding virulence determinates and antibiotic resistance in Staphylococcus aureus isolates from mastitic cows. Alexandria Journal of Veterinary Sciences, 0 (0), -.




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