Genotypic molecular detection of certain genes encoding virulence determinates and antibiotic resistance in Staphylococcus aureus isolates from mastitic cows

Ahmed Lotfy

2025, Vol: 86

86Current Volume Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Original Article

AJVS. 0; 0(0):

Genotypic molecular detection of certain genes encoding virulence determinates and antibiotic resistance in Staphylococcus aureus isolates from mastitic cows

Ahmed Lotfy.

Abstract
A total of 10Staphylococcusaureusisolates were recoveredfrom 117 milk samples whichwere obtained from 93 dairy cows indifferent farms suffered fromclinical mastitis, theseisolateswere phenotypically characterized and identified based on colony morphology andbiochemical tests. Themolecular detection of S. aureus specific sequence gene(Sa),genes encoding clumpingfactor A(clfA), (spa) gene encoding S.aureus protein A and the class 1 integron using PolymeraseChain Reaction were carriedout. All isolateswere negative for the presence of class 1 integronbut yielded an amplicon size of about107 bp in case of S. aureus specific sequence gene (Sa),an amplicon size of about 638bp in case ofclfA gene. Amplification of thespa gene encoding S. aureus protein Awas detected in 9 strains out of 10 (90%)and yielded an amplicon size of about 226bp.

Key words: Molecular detection, Staphylococcus aureus isolates, mastitic cows

	ARTICLE TOOLS
	Abstract
	How to cite this article
	Citation Tools
	Related Records
	Articles by Ahmed Lotfy
	on Google
	on Google Scholar

How to Cite this Article

Pubmed Style

Ahmed Lotfy. Genotypic molecular detection of certain genes encoding virulence determinates and antibiotic resistance in Staphylococcus aureus isolates from mastitic cows. AJVS. 0; 0(0): -.

Web Style

Ahmed Lotfy. Genotypic molecular detection of certain genes encoding virulence determinates and antibiotic resistance in Staphylococcus aureus isolates from mastitic cows. https://www.alexjvs.com/?mno=216450 [Access: May 03, 2025].

AMA (American Medical Association) Style

Ahmed Lotfy. Genotypic molecular detection of certain genes encoding virulence determinates and antibiotic resistance in Staphylococcus aureus isolates from mastitic cows. AJVS. 0; 0(0): -.

Vancouver/ICMJE Style

Ahmed Lotfy. Genotypic molecular detection of certain genes encoding virulence determinates and antibiotic resistance in Staphylococcus aureus isolates from mastitic cows. AJVS. (0), [cited May 03, 2025]; 0(0): -.

Harvard Style

Ahmed Lotfy (0) Genotypic molecular detection of certain genes encoding virulence determinates and antibiotic resistance in Staphylococcus aureus isolates from mastitic cows. AJVS, 0 (0), -.

Turabian Style

Ahmed Lotfy. 0. Genotypic molecular detection of certain genes encoding virulence determinates and antibiotic resistance in Staphylococcus aureus isolates from mastitic cows. Alexandria Journal of Veterinary Sciences, 0 (0), -.

Chicago Style

Ahmed Lotfy. "Genotypic molecular detection of certain genes encoding virulence determinates and antibiotic resistance in Staphylococcus aureus isolates from mastitic cows." Alexandria Journal of Veterinary Sciences 0 (0), -.

MLA (The Modern Language Association) Style

APA (American Psychological Association) Style

Ahmed Lotfy (0) Genotypic molecular detection of certain genes encoding virulence determinates and antibiotic resistance in Staphylococcus aureus isolates from mastitic cows. Alexandria Journal of Veterinary Sciences, 0 (0), -.

About Alexandria Journal of Veterinary Sciences

Contact Information

How to cite this article