E-ISSN 2536-9520 | ISSN 1110-2047
 
2024, Vol: 83
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Original Article 


Preparation and Evaluation of Fluorescein Isothiocyanate-Conjugated Immunoglobulins for the detection of Avian Encephalomyelitis Virus

Shaimaa M. El-gbily, Samy A. Khalil, Mohamed H. Khodeir.


Abstract
To the best of our knowledge, the present study was the first to address the accuracy of using fluorescein-conjugated polyclonal antibodies in routine diagnosis of AEV in Egypt. For raising of anti AEV-hyperimmune sera, a total of 10 male boskat rabbits, 10 male guinea pigs and 2 male goats were used. The Mean SN antibody titers were estimated at weekly intervals and results revealed that they are gradually increased and reached to the peak level at the 5th week post-immunization with a mean titer of 256, 128 and 512 in the sera of rabbits, guinea pigs and goats, respectively. The immunoglobulin contents of the prepared antisera were precipitated by ammonium sulphate and then conjugated with FITC. In order to evaluate the accuracy of the prepared conjugates in diagnostic purposes, direct FAT was carried out on AEV-infected Vero cells using ten-fold serial dilutions of the conjugated immunoglobulins. Rabbit, guinea pig and goat conjugates showed clear positive reactions up to the dilution of 1/100000, 1/10000 and 1/1000000, respectively. The overall findings revealed that accurate and precise diagnosis of AE could be achieved by direct FAT using fluorescein-conjugated anti-rabbit and anti-goat AEV polyclonal antibodies.

Key words: Avian encephalomyelitis virus, Hyperimmune sera, Fluorescien-isothiocyanate.


 
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How to Cite this Article
Pubmed Style

Shaimaa M. El-gbily, Samy A. Khalil, Mohamed H. Khodeir. Preparation and Evaluation of Fluorescein Isothiocyanate-Conjugated Immunoglobulins for the detection of Avian Encephalomyelitis Virus. AJVS. 2016; 51(2): 137-141. doi:10.5455/ajvs.219711

Web Style

Shaimaa M. El-gbily, Samy A. Khalil, Mohamed H. Khodeir. Preparation and Evaluation of Fluorescein Isothiocyanate-Conjugated Immunoglobulins for the detection of Avian Encephalomyelitis Virus. https://www.alexjvs.com/?mno=219711 [Access: September 12, 2024]. doi:10.5455/ajvs.219711

AMA (American Medical Association) Style

Shaimaa M. El-gbily, Samy A. Khalil, Mohamed H. Khodeir. Preparation and Evaluation of Fluorescein Isothiocyanate-Conjugated Immunoglobulins for the detection of Avian Encephalomyelitis Virus. AJVS. 2016; 51(2): 137-141. doi:10.5455/ajvs.219711


Vancouver/ICMJE Style

Shaimaa M. El-gbily, Samy A. Khalil, Mohamed H. Khodeir. Preparation and Evaluation of Fluorescein Isothiocyanate-Conjugated Immunoglobulins for the detection of Avian Encephalomyelitis Virus. AJVS. (2016), [cited September 12, 2024]; 51(2): 137-141. doi:10.5455/ajvs.219711


Harvard Style

Shaimaa M. El-gbily, Samy A. Khalil, Mohamed H. Khodeir (2016) Preparation and Evaluation of Fluorescein Isothiocyanate-Conjugated Immunoglobulins for the detection of Avian Encephalomyelitis Virus. AJVS, 51 (2), 137-141. doi:10.5455/ajvs.219711


Turabian Style

Shaimaa M. El-gbily, Samy A. Khalil, Mohamed H. Khodeir. 2016. Preparation and Evaluation of Fluorescein Isothiocyanate-Conjugated Immunoglobulins for the detection of Avian Encephalomyelitis Virus. Alexandria Journal of Veterinary Sciences, 51 (2), 137-141. doi:10.5455/ajvs.219711


Chicago Style

Shaimaa M. El-gbily, Samy A. Khalil, Mohamed H. Khodeir. "Preparation and Evaluation of Fluorescein Isothiocyanate-Conjugated Immunoglobulins for the detection of Avian Encephalomyelitis Virus." Alexandria Journal of Veterinary Sciences 51 (2016), 137-141. doi:10.5455/ajvs.219711


MLA (The Modern Language Association) Style

Shaimaa M. El-gbily, Samy A. Khalil, Mohamed H. Khodeir. "Preparation and Evaluation of Fluorescein Isothiocyanate-Conjugated Immunoglobulins for the detection of Avian Encephalomyelitis Virus." Alexandria Journal of Veterinary Sciences 51.2 (2016), 137-141. Print. doi:10.5455/ajvs.219711


APA (American Psychological Association) Style

Shaimaa M. El-gbily, Samy A. Khalil, Mohamed H. Khodeir (2016) Preparation and Evaluation of Fluorescein Isothiocyanate-Conjugated Immunoglobulins for the detection of Avian Encephalomyelitis Virus. Alexandria Journal of Veterinary Sciences, 51 (2), 137-141. doi:10.5455/ajvs.219711




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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