E-ISSN 2536-9520 | ISSN 1110-2047
 
2024, Vol: 83
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Original Article 


Phenotypic and Genotypic Characterization of Listeria Monocytogenes Isolated from Different Sources

Ayman M. Kamar, Helmy A. Torky, Samia M. El-Houshy.


Abstract
The aim of this study was to determine the differences concerning phenotypic and genotypic characters of L.monocytogenes isolated from different sources (100 meat, 100 offals, 100 fish and 100 ready to eat foods -RTE- which is represented by luncheon) on the basis of pathogenicity and antibiotic resistance. To achieve this aim, bacteriological examination was done according to ISO 11290 amendment 2004.then confirmed by biochemical reactions and serotyping, then antimicrobial susceptibility testing which indicates the presence of multidrug resistance isolates. Biofilm binding activity of the isolated L.monocytogenes isolates showed that two isolates had strong binding activity, three isolates showed moderate binding activity and one isolate was weak/non biofilm producer. The molecular characterization was done by detection of class1 integron and for pathogenicity by detection of the presence of hlyA gene which encodes listeriolysin of L.monocytogenes then sequencing of the resulted PCR product to determine the difference between the isolates. The confirmation of results was done by SYBR green Real-time PCR.

Key words: Listeria monocytogenes, sequencing, conventional PCR, Realtime PCR, serotyping, biofilm, antimicrobial susceptibility


 
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Pubmed Style

Ayman M. Kamar, Helmy A. Torky, Samia M. El-Houshy. Phenotypic and Genotypic Characterization of Listeria Monocytogenes Isolated from Different Sources. AJVS. 2016; 51(2): 148-157. doi:10.5455/ajvs.222088

Web Style

Ayman M. Kamar, Helmy A. Torky, Samia M. El-Houshy. Phenotypic and Genotypic Characterization of Listeria Monocytogenes Isolated from Different Sources. https://www.alexjvs.com/?mno=222088 [Access: September 13, 2024]. doi:10.5455/ajvs.222088

AMA (American Medical Association) Style

Ayman M. Kamar, Helmy A. Torky, Samia M. El-Houshy. Phenotypic and Genotypic Characterization of Listeria Monocytogenes Isolated from Different Sources. AJVS. 2016; 51(2): 148-157. doi:10.5455/ajvs.222088


Vancouver/ICMJE Style

Ayman M. Kamar, Helmy A. Torky, Samia M. El-Houshy. Phenotypic and Genotypic Characterization of Listeria Monocytogenes Isolated from Different Sources. AJVS. (2016), [cited September 13, 2024]; 51(2): 148-157. doi:10.5455/ajvs.222088


Harvard Style

Ayman M. Kamar, Helmy A. Torky, Samia M. El-Houshy (2016) Phenotypic and Genotypic Characterization of Listeria Monocytogenes Isolated from Different Sources. AJVS, 51 (2), 148-157. doi:10.5455/ajvs.222088


Turabian Style

Ayman M. Kamar, Helmy A. Torky, Samia M. El-Houshy. 2016. Phenotypic and Genotypic Characterization of Listeria Monocytogenes Isolated from Different Sources. Alexandria Journal of Veterinary Sciences, 51 (2), 148-157. doi:10.5455/ajvs.222088


Chicago Style

Ayman M. Kamar, Helmy A. Torky, Samia M. El-Houshy. "Phenotypic and Genotypic Characterization of Listeria Monocytogenes Isolated from Different Sources." Alexandria Journal of Veterinary Sciences 51 (2016), 148-157. doi:10.5455/ajvs.222088


MLA (The Modern Language Association) Style

Ayman M. Kamar, Helmy A. Torky, Samia M. El-Houshy. "Phenotypic and Genotypic Characterization of Listeria Monocytogenes Isolated from Different Sources." Alexandria Journal of Veterinary Sciences 51.2 (2016), 148-157. Print. doi:10.5455/ajvs.222088


APA (American Psychological Association) Style

Ayman M. Kamar, Helmy A. Torky, Samia M. El-Houshy (2016) Phenotypic and Genotypic Characterization of Listeria Monocytogenes Isolated from Different Sources. Alexandria Journal of Veterinary Sciences, 51 (2), 148-157. doi:10.5455/ajvs.222088




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