E-ISSN 2536-9520 | ISSN 1110-2047
 
2024, Vol: 83
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Original Article 


Identification of Single Nucleotide Polymorphisms in BMP 15 Gene in Egyptian buffaloes

Khairy Mohammed El-Bayomi, Iman El-sayed El Araby, Ayman Abdelateef saleh, Hoda zaki othman, Mahmoud salah Eltarabany, ashraf fathy awad, amir hasan Abd El-fattah, mohammed rizk abu el-magd.


Abstract
BMP 15 is a member of transforming growth factor-β (TGF- β) superfamily which plays a crucial role in ovarian follicular development and ovulation rate. In this study a partial sequence of this gene was screened for polymorphisms. PCR amplification of DNA isolated from blood of 100 Egyptian buffaloes hiefers (50 infertile and 50 normal). Revealed two loci, BMP15.1 (334 bp) which includes exon 1 and partial intron 1 and BMP15.2 (272 bp) which spans partial 5UTR and exon 1. Polymorphisms in these two loci were detected by using direct sequencing and single strand conformational polymorphism (SSCP) technique. A single pattern of SSCP was detected. This was confirmed by nucleotide sequencing which revealed absence of single nucleotides polymorphisms (SNPs) or any other type of polymorphisms in the two loci. The sequencing and PCR-SSCP patterns revealed no polymorphism in all studied animals.

Key words: BMP15 gene, polymorphism, Egyptian buffalo, infertility, PCR-SSCP.


 
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Pubmed Style

Khairy Mohammed El-Bayomi, Iman El-sayed El Araby, Ayman Abdelateef saleh, Hoda zaki othman, Mahmoud salah Eltarabany, ashraf fathy awad, amir hasan Abd El-fattah, mohammed rizk abu el-magd. Identification of Single Nucleotide Polymorphisms in BMP 15 Gene in Egyptian buffaloes. AJVS. 0; 0(0): -. doi:10.5455/ajvs.224255

Web Style

Khairy Mohammed El-Bayomi, Iman El-sayed El Araby, Ayman Abdelateef saleh, Hoda zaki othman, Mahmoud salah Eltarabany, ashraf fathy awad, amir hasan Abd El-fattah, mohammed rizk abu el-magd. Identification of Single Nucleotide Polymorphisms in BMP 15 Gene in Egyptian buffaloes. https://www.alexjvs.com/?mno=224255 [Access: September 14, 2024]. doi:10.5455/ajvs.224255

AMA (American Medical Association) Style

Khairy Mohammed El-Bayomi, Iman El-sayed El Araby, Ayman Abdelateef saleh, Hoda zaki othman, Mahmoud salah Eltarabany, ashraf fathy awad, amir hasan Abd El-fattah, mohammed rizk abu el-magd. Identification of Single Nucleotide Polymorphisms in BMP 15 Gene in Egyptian buffaloes. AJVS. 0; 0(0): -. doi:10.5455/ajvs.224255


Vancouver/ICMJE Style

Khairy Mohammed El-Bayomi, Iman El-sayed El Araby, Ayman Abdelateef saleh, Hoda zaki othman, Mahmoud salah Eltarabany, ashraf fathy awad, amir hasan Abd El-fattah, mohammed rizk abu el-magd. Identification of Single Nucleotide Polymorphisms in BMP 15 Gene in Egyptian buffaloes. AJVS. (0), [cited September 14, 2024]; 0(0): -. doi:10.5455/ajvs.224255


Harvard Style

Khairy Mohammed El-Bayomi, Iman El-sayed El Araby, Ayman Abdelateef saleh, Hoda zaki othman, Mahmoud salah Eltarabany, ashraf fathy awad, amir hasan Abd El-fattah, mohammed rizk abu el-magd (0) Identification of Single Nucleotide Polymorphisms in BMP 15 Gene in Egyptian buffaloes. AJVS, 0 (0), -. doi:10.5455/ajvs.224255


Turabian Style

Khairy Mohammed El-Bayomi, Iman El-sayed El Araby, Ayman Abdelateef saleh, Hoda zaki othman, Mahmoud salah Eltarabany, ashraf fathy awad, amir hasan Abd El-fattah, mohammed rizk abu el-magd. 0. Identification of Single Nucleotide Polymorphisms in BMP 15 Gene in Egyptian buffaloes. Alexandria Journal of Veterinary Sciences, 0 (0), -. doi:10.5455/ajvs.224255


Chicago Style

Khairy Mohammed El-Bayomi, Iman El-sayed El Araby, Ayman Abdelateef saleh, Hoda zaki othman, Mahmoud salah Eltarabany, ashraf fathy awad, amir hasan Abd El-fattah, mohammed rizk abu el-magd. "Identification of Single Nucleotide Polymorphisms in BMP 15 Gene in Egyptian buffaloes." Alexandria Journal of Veterinary Sciences 0 (0), -. doi:10.5455/ajvs.224255


MLA (The Modern Language Association) Style

Khairy Mohammed El-Bayomi, Iman El-sayed El Araby, Ayman Abdelateef saleh, Hoda zaki othman, Mahmoud salah Eltarabany, ashraf fathy awad, amir hasan Abd El-fattah, mohammed rizk abu el-magd. "Identification of Single Nucleotide Polymorphisms in BMP 15 Gene in Egyptian buffaloes." Alexandria Journal of Veterinary Sciences 0.0 (0), -. Print. doi:10.5455/ajvs.224255


APA (American Psychological Association) Style

Khairy Mohammed El-Bayomi, Iman El-sayed El Araby, Ayman Abdelateef saleh, Hoda zaki othman, Mahmoud salah Eltarabany, ashraf fathy awad, amir hasan Abd El-fattah, mohammed rizk abu el-magd (0) Identification of Single Nucleotide Polymorphisms in BMP 15 Gene in Egyptian buffaloes. Alexandria Journal of Veterinary Sciences, 0 (0), -. doi:10.5455/ajvs.224255




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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