Production of chicken hyperimmune egg for immunological purposes in animals

Ismail I. Ismail. I. Abo-Ghanema; Madih S. Ibrahim Madih S. Ibrahim; Bahig R. NemettALLAH; Hanan A. Ghoneim

doi:10.5455/ajvs.224376

2025, Vol: 86

86Current Volume Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Original Article

AJVS. 0; 0(0):

doi: 10.5455/ajvs.224376

Production of chicken hyperimmune egg for immunological purposes in animals

Ismail I. Ismail. I. Abo-ghanema, Madih S. Ibrahim Madih S. Ibrahim, Bahig R. Nemettallah, Hanan A. Ghoneim.

Abstract
This study aimed to produce hyperimmune chicken egg against rabbit hemorrhagic disease virus as well as studying immunogenic characters of the extracted IgY using antigen-antibody reaction tests (agar gel immunodiffusion and ELISA). The study was carried out on ten white Hy-Line layers which received initial immunization of 300µl of rabbit hemorrhagic disease virus vaccine subcutaneously followed by three booster doses at time intervals of 2, 5 and 8 weeks after initial immunization. Throughout the experiment (14 weeks), regular blood sampling was made every week with daily collection of egg till further purification and detection of the purity of the isolated IgY by SDS-PAGE. Also, determination of the specificity and titers of purified IgY in hens' sera and their purified egg yolk samples was carried out using agar gel immunodiffusion test and indirect Elisa. Results showed presence of considerable titers of Isolated IgY against rabbit hemorrhagic disease virus. Therefore, this study recommends immunization of hens with rabbit hemorrhagic disease virus for production of egg yolk antibodies against such pathogen.

Key words: Rabbit hemorrhagic disease virus, IgY, Indirect Elisa.

	ARTICLE TOOLS
	Abstract
	How to cite this article
	Citation Tools
	Related Records
	Articles by Ismail I. Ismail. I. Abo-Ghanema Articles by Madih S. Ibrahim Madih S. Ibrahim Articles by Bahig R. NemettALLAH Articles by Hanan A. Ghoneim
	on Google
	on Google Scholar

How to Cite this Article

Pubmed Style

Abo-ghanema IIII, , Nemettallah BR, Ghoneim HA. Production of chicken hyperimmune egg for immunological purposes in animals. AJVS. 0; 0(0): -. doi:10.5455/ajvs.224376

Web Style

Abo-ghanema IIII, , Nemettallah BR, Ghoneim HA. Production of chicken hyperimmune egg for immunological purposes in animals. https://www.alexjvs.com/?mno=224376 [Access: May 03, 2025]. doi:10.5455/ajvs.224376

AMA (American Medical Association) Style

Abo-ghanema IIII, , Nemettallah BR, Ghoneim HA. Production of chicken hyperimmune egg for immunological purposes in animals. AJVS. 0; 0(0): -. doi:10.5455/ajvs.224376

Vancouver/ICMJE Style

Abo-ghanema IIII, , Nemettallah BR, Ghoneim HA. Production of chicken hyperimmune egg for immunological purposes in animals. AJVS. (0), [cited May 03, 2025]; 0(0): -. doi:10.5455/ajvs.224376

Harvard Style

Abo-ghanema, I. I. I. I., , Nemettallah, . B. R. & Ghoneim, . H. A. (0) Production of chicken hyperimmune egg for immunological purposes in animals. AJVS, 0 (0), -. doi:10.5455/ajvs.224376

Turabian Style

Abo-ghanema, Ismail I. Ismail. I., Madih S. Ibrahim Madih S. Ibrahim, Bahig R. Nemettallah, and Hanan A. Ghoneim. 0. Production of chicken hyperimmune egg for immunological purposes in animals. Alexandria Journal of Veterinary Sciences, 0 (0), -. doi:10.5455/ajvs.224376

Chicago Style

Abo-ghanema, Ismail I. Ismail. I., Madih S. Ibrahim Madih S. Ibrahim, Bahig R. Nemettallah, and Hanan A. Ghoneim. "Production of chicken hyperimmune egg for immunological purposes in animals." Alexandria Journal of Veterinary Sciences 0 (0), -. doi:10.5455/ajvs.224376

MLA (The Modern Language Association) Style

APA (American Psychological Association) Style

Abo-ghanema, I. I. I. I., , Nemettallah, . B. R. & Ghoneim, . H. A. (0) Production of chicken hyperimmune egg for immunological purposes in animals. Alexandria Journal of Veterinary Sciences, 0 (0), -. doi:10.5455/ajvs.224376

About Alexandria Journal of Veterinary Sciences

Contact Information

How to cite this article