E-ISSN 2536-9520 | ISSN 1110-2047
 
2024, Vol: 83
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Original Article 


Production of chicken hyperimmune egg for immunological purposes in animals

Ismail I. Ismail. I. Abo-Ghanema, Madih S. Ibrahim Madih S. Ibrahim, Bahig R. NemettALLAH, Hanan A. Ghoneim.


Abstract
This study aimed to produce hyperimmune chicken egg against rabbit hemorrhagic disease virus as well as studying immunogenic characters of the extracted IgY using antigen-antibody reaction tests (agar gel immunodiffusion and ELISA). The study was carried out on ten white Hy-Line layers which received initial immunization of 300µl of rabbit hemorrhagic disease virus vaccine subcutaneously followed by three booster doses at time intervals of 2, 5 and 8 weeks after initial immunization. Throughout the experiment (14 weeks), regular blood sampling was made every week with daily collection of egg till further purification and detection of the purity of the isolated IgY by SDS-PAGE. Also, determination of the specificity and titers of purified IgY in hens' sera and their purified egg yolk samples was carried out using agar gel immunodiffusion test and indirect Elisa. Results showed presence of considerable titers of Isolated IgY against rabbit hemorrhagic disease virus. Therefore, this study recommends immunization of hens with rabbit hemorrhagic disease virus for production of egg yolk antibodies against such pathogen.

Key words: Rabbit hemorrhagic disease virus, IgY, Indirect Elisa.


 
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How to Cite this Article
Pubmed Style

Ismail I. Ismail. I. Abo-Ghanema , Madih S. Ibrahim Madih S. Ibrahim, Bahig R. NemettALLAH, Hanan A. Ghoneim. Production of chicken hyperimmune egg for immunological purposes in animals. AJVS. 0; 0(0): -. doi:10.5455/ajvs.224376

Web Style

Ismail I. Ismail. I. Abo-Ghanema , Madih S. Ibrahim Madih S. Ibrahim, Bahig R. NemettALLAH, Hanan A. Ghoneim. Production of chicken hyperimmune egg for immunological purposes in animals. https://www.alexjvs.com/?mno=224376 [Access: September 13, 2024]. doi:10.5455/ajvs.224376

AMA (American Medical Association) Style

Ismail I. Ismail. I. Abo-Ghanema , Madih S. Ibrahim Madih S. Ibrahim, Bahig R. NemettALLAH, Hanan A. Ghoneim. Production of chicken hyperimmune egg for immunological purposes in animals. AJVS. 0; 0(0): -. doi:10.5455/ajvs.224376


Vancouver/ICMJE Style

Ismail I. Ismail. I. Abo-Ghanema , Madih S. Ibrahim Madih S. Ibrahim, Bahig R. NemettALLAH, Hanan A. Ghoneim. Production of chicken hyperimmune egg for immunological purposes in animals. AJVS. (0), [cited September 13, 2024]; 0(0): -. doi:10.5455/ajvs.224376


Harvard Style

Ismail I. Ismail. I. Abo-Ghanema , Madih S. Ibrahim Madih S. Ibrahim, Bahig R. NemettALLAH, Hanan A. Ghoneim (0) Production of chicken hyperimmune egg for immunological purposes in animals. AJVS, 0 (0), -. doi:10.5455/ajvs.224376


Turabian Style

Ismail I. Ismail. I. Abo-Ghanema , Madih S. Ibrahim Madih S. Ibrahim, Bahig R. NemettALLAH, Hanan A. Ghoneim. 0. Production of chicken hyperimmune egg for immunological purposes in animals. Alexandria Journal of Veterinary Sciences, 0 (0), -. doi:10.5455/ajvs.224376


Chicago Style

Ismail I. Ismail. I. Abo-Ghanema , Madih S. Ibrahim Madih S. Ibrahim, Bahig R. NemettALLAH, Hanan A. Ghoneim. "Production of chicken hyperimmune egg for immunological purposes in animals." Alexandria Journal of Veterinary Sciences 0 (0), -. doi:10.5455/ajvs.224376


MLA (The Modern Language Association) Style

Ismail I. Ismail. I. Abo-Ghanema , Madih S. Ibrahim Madih S. Ibrahim, Bahig R. NemettALLAH, Hanan A. Ghoneim. "Production of chicken hyperimmune egg for immunological purposes in animals." Alexandria Journal of Veterinary Sciences 0.0 (0), -. Print. doi:10.5455/ajvs.224376


APA (American Psychological Association) Style

Ismail I. Ismail. I. Abo-Ghanema , Madih S. Ibrahim Madih S. Ibrahim, Bahig R. NemettALLAH, Hanan A. Ghoneim (0) Production of chicken hyperimmune egg for immunological purposes in animals. Alexandria Journal of Veterinary Sciences, 0 (0), -. doi:10.5455/ajvs.224376




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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