E-ISSN 2536-9520 | ISSN 1110-2047
 
2025, Vol: 85
85Current Volume Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Original Article
Online Published: 11 Aug 2016
 


Isolation and Molecular Characterization of Newcastle Disease Virus Genotype VI from Pigeons in Egypt

Moustafa M. El-shazly, Basem M. Ahmed, Ahmed A. El-sanousi, Youssef I. Youssef.


Abstract
A pool of brain sample was collected from three pigeons showing torticollis and whitish green diarrhea in Desouk, Kafr-elshiekh Province, Egypt,during January, 2014. Virus Isolation has been pursued by inoculation of brain homogenate into the allantoic sac of 9 day old specific pathogen free (SPF) embryonated chicken eggs and incubated at 37oC for 4 days. Identification of the isolate was carried out by haemagglutination inhibition test using specific polyclonal antisera against NDV and AIV ( subtype H5 and H9), RT-PCR and confirmed by sequencing. Partial deduced amino acid sequence of the fusion protein at the cleavage site revealed that the isolate possessed the motif 112 K-R-Q-K-R-F117 indicating velogenic entity and phylogenetic analysis showed clustering of the study sequence with other genotype IV F gene sequences available in GenBank.The study indicated that pigeons may play epidemiological role in the introduction of a new NDV genotype VI to commercials chicken.

Key words: Newcastle disease virus; Velogenic; pigeon; Genotype IV; Egypt


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Moustafa M. El-Shazly
Articles by Basem M. Ahmed
Articles by Ahmed A. El-Sanousi
Articles by Youssef I. Youssef
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

El-shazly MM, Ahmed BM, El-sanousi AA, Youssef YI. Isolation and Molecular Characterization of Newcastle Disease Virus Genotype VI from Pigeons in Egypt. AJVS. 2016; 50(1): 145-149. doi:10.5455/ajvs.230284

Web Style

El-shazly MM, Ahmed BM, El-sanousi AA, Youssef YI. Isolation and Molecular Characterization of Newcastle Disease Virus Genotype VI from Pigeons in Egypt. https://www.alexjvs.com/?mno=230284 [Access: March 26, 2025]. doi:10.5455/ajvs.230284

AMA (American Medical Association) Style

El-shazly MM, Ahmed BM, El-sanousi AA, Youssef YI. Isolation and Molecular Characterization of Newcastle Disease Virus Genotype VI from Pigeons in Egypt. AJVS. 2016; 50(1): 145-149. doi:10.5455/ajvs.230284


Vancouver/ICMJE Style

El-shazly MM, Ahmed BM, El-sanousi AA, Youssef YI. Isolation and Molecular Characterization of Newcastle Disease Virus Genotype VI from Pigeons in Egypt. AJVS. (2016), [cited March 26, 2025]; 50(1): 145-149. doi:10.5455/ajvs.230284


Harvard Style

El-shazly, M. M., Ahmed, . B. M., El-sanousi, . A. A. & Youssef, . Y. I. (2016) Isolation and Molecular Characterization of Newcastle Disease Virus Genotype VI from Pigeons in Egypt. AJVS, 50 (1), 145-149. doi:10.5455/ajvs.230284


Turabian Style

El-shazly, Moustafa M., Basem M. Ahmed, Ahmed A. El-sanousi, and Youssef I. Youssef. 2016. Isolation and Molecular Characterization of Newcastle Disease Virus Genotype VI from Pigeons in Egypt. Alexandria Journal of Veterinary Sciences, 50 (1), 145-149. doi:10.5455/ajvs.230284


Chicago Style

El-shazly, Moustafa M., Basem M. Ahmed, Ahmed A. El-sanousi, and Youssef I. Youssef. "Isolation and Molecular Characterization of Newcastle Disease Virus Genotype VI from Pigeons in Egypt." Alexandria Journal of Veterinary Sciences 50 (2016), 145-149. doi:10.5455/ajvs.230284


MLA (The Modern Language Association) Style

El-shazly, Moustafa M., Basem M. Ahmed, Ahmed A. El-sanousi, and Youssef I. Youssef. "Isolation and Molecular Characterization of Newcastle Disease Virus Genotype VI from Pigeons in Egypt." Alexandria Journal of Veterinary Sciences 50.1 (2016), 145-149. Print. doi:10.5455/ajvs.230284


APA (American Psychological Association) Style

El-shazly, M. M., Ahmed, . B. M., El-sanousi, . A. A. & Youssef, . Y. I. (2016) Isolation and Molecular Characterization of Newcastle Disease Virus Genotype VI from Pigeons in Egypt. Alexandria Journal of Veterinary Sciences, 50 (1), 145-149. doi:10.5455/ajvs.230284




Most Viewed Articles
Most Accessed Articles

  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

    •
    Most Downloaded
    Top Downloaded Articles

  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Effect of Excess Fluoride on Reproductive Potentials in Farm Animals (Ovine)
    Ghada H. Abdel-Rahman, Hanaa A. El-Hallawany, Dohreig RA.
    AJVS. 2018; 57(2): 41-57
    » Abstract » doi: 10.5455/ajvs.296364

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract » doi: 10.5455/ajvs.48232

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract » doi: 10.5455/ajvs.47290

    •
    Most Cited Articles
    Most Cited Articles

    About Alexandria Journal of Veterinary Sciences

    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



    For best results, please use Internet Explorer or Google Chrome.

    Contact Information

    Faculty of Veterinary Medicine
    Alexandria University
    P.O.: 21944
    Abees 10th district, Alexandria
    Egypt.

    email: ajvs@alexu.edu.eg
    POLICIES & JOURNAL LINKS
    Author Login
    Reviewer Login
    About Publisher
    Advertising Policy
    Author's Rights and Obligations
    Conflict of Interest Policy
    Copyright Information
    Digital Archiving & Preservation Policies
    Editorial Policies
    Peer Review Policy
    Editorial & Peer Review Process
    License Information
    Open Access Policy
    Plagiarism Policy
    Privacy Policy
    Protection of Research Participants (Statement On Human And Animal Rights)
    Publication Ethics and Publication Malpractice Statement
    Corrections, Retractions & Expressions of Concern
    Self-Archiving Policies
    Statement of Informed Consent
    Terms of Use