E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Sanitary condition of some raw edible beef offal

Asmaa M. Khalil, Mohamed M. Mousa, Engy F. El-Bahy.


Abstract
A total of 100 random samples of some edible beef offal represented by liver, heart, lung and spleen (25 of each) were subjected to bacteriological examination for Mesophilic bacterial count, Enterobacteriaceae count, Coliforms count, Staphylococcal count, the incidence of S. aureus and detection of enterotoxin genes of S. aureus to evaluate their quality. The mean value of Mesophilic bacterial count / g of liver, heart, lung and spleen were 3.9×104, 3.6×104, 3.03×104 and 5.4×104 cfu/g, respectively. The mean value of Enterobacteriaceae count / g of liver, heart, lung and spleen were 2.3×104, 1.4×104, 1.2×104 and 1.5×104 cfu/g, respectively. The mean value of Coliforms count / g of liver, heart, lung and spleen were 6.4×102, 5.6×102, 6.1×102 and 8.2×102 MPN/g, respectively. The mean value of Staphylococci count / g of liver, heart, lung and spleen were 1.1×102, 1.1×102, 1.3×102 and 1.7×102 cfu/g, respectively. The incidence of S. aureus isolated from examined liver, heart, lung and spleen samples were 36%, 44%, 64% and 32%, respectively. Analysis of the PCR products obtained from amplification reaction of extracted DNA from 10 identified S. aureus strains in this study for detection of enterotoxins genes by agarose gel electrophoresis revealed that the specific band for SEA gene (120bp) was detected in 3(30%) one from liver, heart and spleen out of 10 isolates, while the specific band for SEB gene (478bp) was not detected in any isolate. The specific band for SEC gene (257bp) was detected in 1(10%) from lung out of 10 isolates, also the specific band for SED gene (317bp) was detected in 1(10%) from lung out of 10 isolates. The specific band for SEA, SEC genes (120bp, 257bp) was detected in 1(10%) from spleen out of 10 isolates.

Key words: Enterobacteriaceae, S. aureus, Coliforms, edible beef offal, Enterotoxin genes.


 
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How to Cite this Article
Pubmed Style

Khalil AM, Mousa MM, El-Bahy EF. Sanitary condition of some raw edible beef offal. AJVS. 0; 0(0): -. doi:10.5455/ajvs.234694


Web Style

Khalil AM, Mousa MM, El-Bahy EF. Sanitary condition of some raw edible beef offal. https://www.alexjvs.com/?mno=234694 [Access: September 12, 2024]. doi:10.5455/ajvs.234694


AMA (American Medical Association) Style

Khalil AM, Mousa MM, El-Bahy EF. Sanitary condition of some raw edible beef offal. AJVS. 0; 0(0): -. doi:10.5455/ajvs.234694



Vancouver/ICMJE Style

Khalil AM, Mousa MM, El-Bahy EF. Sanitary condition of some raw edible beef offal. AJVS. (0), [cited September 12, 2024]; 0(0): -. doi:10.5455/ajvs.234694



Harvard Style

Khalil, A. M., Mousa, . M. M. & El-Bahy, . E. F. (0) Sanitary condition of some raw edible beef offal. AJVS, 0 (0), -. doi:10.5455/ajvs.234694



Turabian Style

Khalil, Asmaa M., Mohamed M. Mousa, and Engy F. El-Bahy. 0. Sanitary condition of some raw edible beef offal. Alexandria Journal of Veterinary Sciences, 0 (0), -. doi:10.5455/ajvs.234694



Chicago Style

Khalil, Asmaa M., Mohamed M. Mousa, and Engy F. El-Bahy. "Sanitary condition of some raw edible beef offal." Alexandria Journal of Veterinary Sciences 0 (0), -. doi:10.5455/ajvs.234694



MLA (The Modern Language Association) Style

Khalil, Asmaa M., Mohamed M. Mousa, and Engy F. El-Bahy. "Sanitary condition of some raw edible beef offal." Alexandria Journal of Veterinary Sciences 0.0 (0), -. Print. doi:10.5455/ajvs.234694



APA (American Psychological Association) Style

Khalil, A. M., Mousa, . M. M. & El-Bahy, . E. F. (0) Sanitary condition of some raw edible beef offal. Alexandria Journal of Veterinary Sciences, 0 (0), -. doi:10.5455/ajvs.234694





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