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Original Article 


Effect of Different Semen Extenders on Post-Thawing Activity of Arabian Stallion Spermatozoa

Aly A. El-sharkawy, Sayed A. Hattab, Ismail I. Ghanema, Mohamed N. El-garhy, Mahmoud Kh. Soliman, Diya A. El-badry.


Abstract
This study aimed to compare the efficiency of four cryodiluents on post-thawing activity of Arabian stallion spermatozoa. Semen samples were collected from 10 Arabian stallions, diluted with modified INRA-82 (m-INRA), modified Kenney’s (m-Kenney) and or Eqi-Pro® diluents and subjected to cryopreservation. Post-thaw motility, membrane, acrosomal and mitochondrial integrities were evaluated. The results revealed the superiority of m-INRA over m-Kenney and or Eqi-Pro® in terms of post-thaw motility (52.50 ± 2.27 vs. 43.00 ± 2.13 and 41.50 ± 2.59%, respectively), viability index (134.25 ± 5.99 vs. 97.50 ± 6.40 and 94.75 ± 7.33, respectively), sperm membrane (46.60 ± 2.05 vs. 36.70 ± 1.73 and 36.60 ± 2.09%, respectively) and acrosome integrities (54.50 ± 1.30 vs. 43.00 ± 1.86 and 43.5 ±1.86%). Activities of stallion sperm mitochondria were better preserved using m-INRA as cryodiluent. Using Kenney’s extender resulted in the lowest sperm motility and integrities of acrosome and membranes (33.00 ± 2.13, 35.50 ± 1.38 and 29.80 ± 1.14%, respectively). In conclusion, the motility as well as functional integrities of Arabian stallion spermatozoa could be enhanced using m-INRA as a cryodiluent.

Key words: Stallion-semen-cryopreservation-diluent-INRA-Kenny’s- Eqi-Pro


 
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How to Cite this Article
Pubmed Style

El-sharkawy AA, Hattab SA, Ghanema II, El-garhy MN, Soliman MK, El-badry DA. Effect of Different Semen Extenders on Post-Thawing Activity of Arabian Stallion Spermatozoa. AJVS. 2016; 51(2): 178-182. doi:10.5455/ajvs.241513

Web Style

El-sharkawy AA, Hattab SA, Ghanema II, El-garhy MN, Soliman MK, El-badry DA. Effect of Different Semen Extenders on Post-Thawing Activity of Arabian Stallion Spermatozoa. https://www.alexjvs.com/?mno=241513 [Access: March 26, 2025]. doi:10.5455/ajvs.241513

AMA (American Medical Association) Style

El-sharkawy AA, Hattab SA, Ghanema II, El-garhy MN, Soliman MK, El-badry DA. Effect of Different Semen Extenders on Post-Thawing Activity of Arabian Stallion Spermatozoa. AJVS. 2016; 51(2): 178-182. doi:10.5455/ajvs.241513


Vancouver/ICMJE Style

El-sharkawy AA, Hattab SA, Ghanema II, El-garhy MN, Soliman MK, El-badry DA. Effect of Different Semen Extenders on Post-Thawing Activity of Arabian Stallion Spermatozoa. AJVS. (2016), [cited March 26, 2025]; 51(2): 178-182. doi:10.5455/ajvs.241513


Harvard Style

El-sharkawy, A. A., Hattab, . S. A., Ghanema, . I. I., El-garhy, . M. N., Soliman, . M. K. & El-badry, . D. A. (2016) Effect of Different Semen Extenders on Post-Thawing Activity of Arabian Stallion Spermatozoa. AJVS, 51 (2), 178-182. doi:10.5455/ajvs.241513


Turabian Style

El-sharkawy, Aly A., Sayed A. Hattab, Ismail I. Ghanema, Mohamed N. El-garhy, Mahmoud Kh. Soliman, and Diya A. El-badry. 2016. Effect of Different Semen Extenders on Post-Thawing Activity of Arabian Stallion Spermatozoa. Alexandria Journal of Veterinary Sciences, 51 (2), 178-182. doi:10.5455/ajvs.241513


Chicago Style

El-sharkawy, Aly A., Sayed A. Hattab, Ismail I. Ghanema, Mohamed N. El-garhy, Mahmoud Kh. Soliman, and Diya A. El-badry. "Effect of Different Semen Extenders on Post-Thawing Activity of Arabian Stallion Spermatozoa." Alexandria Journal of Veterinary Sciences 51 (2016), 178-182. doi:10.5455/ajvs.241513


MLA (The Modern Language Association) Style

El-sharkawy, Aly A., Sayed A. Hattab, Ismail I. Ghanema, Mohamed N. El-garhy, Mahmoud Kh. Soliman, and Diya A. El-badry. "Effect of Different Semen Extenders on Post-Thawing Activity of Arabian Stallion Spermatozoa." Alexandria Journal of Veterinary Sciences 51.2 (2016), 178-182. Print. doi:10.5455/ajvs.241513


APA (American Psychological Association) Style

El-sharkawy, A. A., Hattab, . S. A., Ghanema, . I. I., El-garhy, . M. N., Soliman, . M. K. & El-badry, . D. A. (2016) Effect of Different Semen Extenders on Post-Thawing Activity of Arabian Stallion Spermatozoa. Alexandria Journal of Veterinary Sciences, 51 (2), 178-182. doi:10.5455/ajvs.241513




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    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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