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Original Article
Online Published: 22 Nov 2016
 


Assessment oF Phylogenetic Relationship and Genetic Variability among Some Duck Breeds Using RAPD-PCR as Molecular Markers

Iman E. El-Araby and Ayman A. Saleh.


Abstract
RAPD-PCR was utilized to assess the phylogenetic relationship and genetic variation among four duck breeds; Muscovy, Sudani, Pekin and Moulard, which reared in Egypt. A total of 13 arbitrary short primers were used and they generated polymorphic, reproducible and scoreable bands. These primers produced 92 bands Out of these scored bands, 37 (40.2%) were recognized as monomorphic and 55 (59.8%) as polymorphic bands. OPC-1 produced the highest percentage (100%) of polymorphic bands, while OPA-18 generated the lowest percentage (37.5%) of polymorphic bands. Interbreed of band sharing frequencies ranged from 0.79±0.06 between Muscovy-Moulard to 0.65±0.06 between Sudani-Pekin. Three primers (OPA-12, OPA-18 and OPA-20) in Sudani, six primers (OPA-08, OPA-15, OPA-17, OPB-15, OPC-01 and OPF-09) in Pekin were specified for these breeds. The highest genetic distance 6.32 was found between Sudani-Pekin, while the lowest genetic distances 4.90 was found between Muscovy - Moulard. The study suggests the ability of RAPD- PCR to estimate the genetic distance and reflect the genetic changes and relations among the studied duck breeds.
Key words: Duck, RAPD- PCR, genetic variability, phylogenetic.

Key words: RAPD-PCR, Duck, Muscovy, Sudani, Pekin and Moulard


 
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Pubmed Style

Iman E. El-Araby and Ayman A. Saleh. Assessment oF Phylogenetic Relationship and Genetic Variability among Some Duck Breeds Using RAPD-PCR as Molecular Markers. AJVS. 2016; 51(1): 174-179. doi:10.5455/ajvs.246790

Web Style

Iman E. El-Araby and Ayman A. Saleh. Assessment oF Phylogenetic Relationship and Genetic Variability among Some Duck Breeds Using RAPD-PCR as Molecular Markers. https://www.alexjvs.com/?mno=250085 [Access: December 01, 2024]. doi:10.5455/ajvs.246790

AMA (American Medical Association) Style

Iman E. El-Araby and Ayman A. Saleh. Assessment oF Phylogenetic Relationship and Genetic Variability among Some Duck Breeds Using RAPD-PCR as Molecular Markers. AJVS. 2016; 51(1): 174-179. doi:10.5455/ajvs.246790


Vancouver/ICMJE Style

Iman E. El-Araby and Ayman A. Saleh. Assessment oF Phylogenetic Relationship and Genetic Variability among Some Duck Breeds Using RAPD-PCR as Molecular Markers. AJVS. (2016), [cited December 01, 2024]; 51(1): 174-179. doi:10.5455/ajvs.246790


Harvard Style

Iman E. El-Araby and Ayman A. Saleh (2016) Assessment oF Phylogenetic Relationship and Genetic Variability among Some Duck Breeds Using RAPD-PCR as Molecular Markers. AJVS, 51 (1), 174-179. doi:10.5455/ajvs.246790


Turabian Style

Iman E. El-Araby and Ayman A. Saleh. 2016. Assessment oF Phylogenetic Relationship and Genetic Variability among Some Duck Breeds Using RAPD-PCR as Molecular Markers. Alexandria Journal of Veterinary Sciences, 51 (1), 174-179. doi:10.5455/ajvs.246790


Chicago Style

Iman E. El-Araby and Ayman A. Saleh. "Assessment oF Phylogenetic Relationship and Genetic Variability among Some Duck Breeds Using RAPD-PCR as Molecular Markers." Alexandria Journal of Veterinary Sciences 51 (2016), 174-179. doi:10.5455/ajvs.246790


MLA (The Modern Language Association) Style

Iman E. El-Araby and Ayman A. Saleh. "Assessment oF Phylogenetic Relationship and Genetic Variability among Some Duck Breeds Using RAPD-PCR as Molecular Markers." Alexandria Journal of Veterinary Sciences 51.1 (2016), 174-179. Print. doi:10.5455/ajvs.246790


APA (American Psychological Association) Style

Iman E. El-Araby and Ayman A. Saleh (2016) Assessment oF Phylogenetic Relationship and Genetic Variability among Some Duck Breeds Using RAPD-PCR as Molecular Markers. Alexandria Journal of Veterinary Sciences, 51 (1), 174-179. doi:10.5455/ajvs.246790




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