E-ISSN 2536-9520 | ISSN 1110-2047
 
2024, Vol: 83
83Current Volume Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Original Article
Online Published: 07 Jan 2017
 


Molecular Characterization of Vibrio Harveyi in Diseased Shrimp

Helmy A. Torky, Gaber S. Abdellrazeq, Mona M. Hussein, Nourhan H. Ghanem.


Abstract
The objective of this study was to characterize Vibrio harveyi phenotypically and by molecular methods. A total number of 420 shrimp post larvae samples were collected, 280 samples of diseased cultured shrimp and 140 samples of apparently healthy marine shrimp. The samples were subjected to bacteriological examination. Thirty three (11.7%) of cultured shrimp samples and 4 (2.8%) of marine shrimp samples were phenotypically positive for V. harveyi. Species - specific gene (toxRgene) and two virulence associated genes (vhh -Vibrio harveyi haemolysin, and partial hly- haemolysin gene) were investigated using conventional PCR.
This study proved that using of partial haemolysin gene in molecular characterization of V. harveyi is the fastest and most accurate method to identify all isolates of V. harveyi (highly pathogenic, moderately pathogenic and non-pathogenic strains) due to the presence of a single copy of haemolysin gene encoded in all isolates, although it is not in the same locus in the genome.

Key words: Molecular Characterization, Vibrio harveyi, Diseased Shrimp.


 
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How to Cite this Article
Pubmed Style

Helmy A. Torky, Gaber S. Abdellrazeq, Mona M. Hussein, Nourhan H. Ghanem. Molecular Characterization of Vibrio Harveyi in Diseased Shrimp. AJVS. 2016; 51(2): 358-366. doi:10.5455/ajvs.206029

Web Style

Helmy A. Torky, Gaber S. Abdellrazeq, Mona M. Hussein, Nourhan H. Ghanem. Molecular Characterization of Vibrio Harveyi in Diseased Shrimp. https://www.alexjvs.com/?mno=255141 [Access: September 12, 2024]. doi:10.5455/ajvs.206029

AMA (American Medical Association) Style

Helmy A. Torky, Gaber S. Abdellrazeq, Mona M. Hussein, Nourhan H. Ghanem. Molecular Characterization of Vibrio Harveyi in Diseased Shrimp. AJVS. 2016; 51(2): 358-366. doi:10.5455/ajvs.206029


Vancouver/ICMJE Style

Helmy A. Torky, Gaber S. Abdellrazeq, Mona M. Hussein, Nourhan H. Ghanem. Molecular Characterization of Vibrio Harveyi in Diseased Shrimp. AJVS. (2016), [cited September 12, 2024]; 51(2): 358-366. doi:10.5455/ajvs.206029


Harvard Style

Helmy A. Torky, Gaber S. Abdellrazeq, Mona M. Hussein, Nourhan H. Ghanem (2016) Molecular Characterization of Vibrio Harveyi in Diseased Shrimp. AJVS, 51 (2), 358-366. doi:10.5455/ajvs.206029


Turabian Style

Helmy A. Torky, Gaber S. Abdellrazeq, Mona M. Hussein, Nourhan H. Ghanem. 2016. Molecular Characterization of Vibrio Harveyi in Diseased Shrimp. Alexandria Journal of Veterinary Sciences, 51 (2), 358-366. doi:10.5455/ajvs.206029


Chicago Style

Helmy A. Torky, Gaber S. Abdellrazeq, Mona M. Hussein, Nourhan H. Ghanem. "Molecular Characterization of Vibrio Harveyi in Diseased Shrimp." Alexandria Journal of Veterinary Sciences 51 (2016), 358-366. doi:10.5455/ajvs.206029


MLA (The Modern Language Association) Style

Helmy A. Torky, Gaber S. Abdellrazeq, Mona M. Hussein, Nourhan H. Ghanem. "Molecular Characterization of Vibrio Harveyi in Diseased Shrimp." Alexandria Journal of Veterinary Sciences 51.2 (2016), 358-366. Print. doi:10.5455/ajvs.206029


APA (American Psychological Association) Style

Helmy A. Torky, Gaber S. Abdellrazeq, Mona M. Hussein, Nourhan H. Ghanem (2016) Molecular Characterization of Vibrio Harveyi in Diseased Shrimp. Alexandria Journal of Veterinary Sciences, 51 (2), 358-366. doi:10.5455/ajvs.206029




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