Genetic Diversity of Pasteurella Multocida Isolated from Different Animal Species

Samy A. Khalil; Ahmed A. Bkheet; Fayez A. Gad Allah

doi:10.5455/ajvs.263961

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Original Article

AJVS. 2017; 54(1): 173-178

doi: 10.5455/ajvs.263961

Genetic Diversity of Pasteurella Multocida Isolated from Different Animal Species

Samy A. Khalil, Ahmed A. Bkheet, Fayez A. Gad Allah.

Abstract
In this study, a total number of animal samples (583) were collected from cattle (170) , sheep (221) and goats (192) suffering from respiratory manifestations (freshly dead and slaughtered). Nasal swabs (169) were collected from cattle (43), sheep (67) and goats (59) . Blood samples (312) were collected from cattle (95) , sheep (112) and goats (105) and lung and spleen tissues (102) were collected from cattle (32) ,sheep (42) and goats (28) . Samples collected for bacteriological isolation and identification of P. multocida. After that confirmation of identification by using PM-PCR, and multiplex-PCR for detection of the virulence factors genes, DNA nucleotides sequencing of these genes were done for detection of the genetic diversity.
The prevalence of P.multocida isolated from all samples from Cattle, Sheep and Goats is 1.76%, 1.8%, 1.56% respectively. While, P. multocida not found in all blood samples collected from cattle, sheep and goat.
PM-PCR and multiplex-PCR confirmed presence of genes under examination.
DNA nucleotide sequencing and analysis of sequences obtained for the 5 genes under investigation Concluded that: P. multocida isolates had no genetic diversity of oma 87 gene, ptf A gene, phf A, tox A and nan B among cattle, sheep and goats.

Key words: Genetic diversity, Pasteurella multocida, virulence factors genes.

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Pubmed Style

Khalil SA, Bkheet AA, Allah FAG. Genetic Diversity of Pasteurella Multocida Isolated from Different Animal Species. AJVS. 2017; 54(1): 173-178. doi:10.5455/ajvs.263961

Web Style

Khalil SA, Bkheet AA, Allah FAG. Genetic Diversity of Pasteurella Multocida Isolated from Different Animal Species. https://www.alexjvs.com/?mno=263961 [Access: May 04, 2025]. doi:10.5455/ajvs.263961

AMA (American Medical Association) Style

Khalil SA, Bkheet AA, Allah FAG. Genetic Diversity of Pasteurella Multocida Isolated from Different Animal Species. AJVS. 2017; 54(1): 173-178. doi:10.5455/ajvs.263961

Vancouver/ICMJE Style

Khalil SA, Bkheet AA, Allah FAG. Genetic Diversity of Pasteurella Multocida Isolated from Different Animal Species. AJVS. (2017), [cited May 04, 2025]; 54(1): 173-178. doi:10.5455/ajvs.263961

Harvard Style

Khalil, S. A., Bkheet, . A. A. & Allah, . F. A. G. (2017) Genetic Diversity of Pasteurella Multocida Isolated from Different Animal Species. AJVS, 54 (1), 173-178. doi:10.5455/ajvs.263961

Turabian Style

Khalil, Samy A., Ahmed A. Bkheet, and Fayez A. Gad Allah. 2017. Genetic Diversity of Pasteurella Multocida Isolated from Different Animal Species. Alexandria Journal of Veterinary Sciences, 54 (1), 173-178. doi:10.5455/ajvs.263961

Chicago Style

Khalil, Samy A., Ahmed A. Bkheet, and Fayez A. Gad Allah. "Genetic Diversity of Pasteurella Multocida Isolated from Different Animal Species." Alexandria Journal of Veterinary Sciences 54 (2017), 173-178. doi:10.5455/ajvs.263961

MLA (The Modern Language Association) Style

APA (American Psychological Association) Style

Khalil, S. A., Bkheet, . A. A. & Allah, . F. A. G. (2017) Genetic Diversity of Pasteurella Multocida Isolated from Different Animal Species. Alexandria Journal of Veterinary Sciences, 54 (1), 173-178. doi:10.5455/ajvs.263961

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