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Original Article
Online Published: 09 Mar 2014
 


Evaluation of a Rapid Immunochromatographic Test for Detection of Brucella Abortus Antibodies in Egyptian Cattle Sera and Milk

Tharwat M. Elshemey, Amir H. Abd-Elrahman.


Abstract
Rapid diagnostic tests are needed to facilitate diagnosis and control of brucellosis. Sero-epidemiology of brucellosis is currently done by employing the Rose Bengal test (RBT). Also the world organization for animal health (OIE) has approved an indirect ELISA for testing serum and milk. The Immuno-Chromatographic brucellosis test (ICT) is a rapid, card-based immunochromatographic test for detection of antibodies directed against B. abortus antigens. To the best of our knowledge, ICT has not been used for the diagnosis of brucellosis in cattle yet. The objective of the study was to evaluate the performance of the ICT brucellosis test for the diagnosis of B. abortus in cattle sera versus RBT and ELISA as a gold standard, Also evaluation of ICT brucellosis test efficacy for detection of antibodies against B. Abortus in milk samples. 94, 90.36 and 84.3% of sera samples were positive by RBT, ICT and ELISA respectively. The ICT had 94.44 % sensitivity and 100 % specificity versus RBT and ELISA as a gold standard. The kappa value between ICT and ELISA was 0.76. Also ICT detected antibodies against B. abortus in serum and milk samples of 7 cows while a recently calved cow showed antibodies in the milk sample only. In conclusion, ICT provides a handy and accurate tool for the rapid detection of antibodies to B. abortus in cattle sera and milk.

Key words: Brucella abortus, RBT, Immunochromatographic test, ELISA


 
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Pubmed Style

Tharwat M. Elshemey, Amir H. Abd-Elrahman. Evaluation of a Rapid Immunochromatographic Test for Detection of Brucella Abortus Antibodies in Egyptian Cattle Sera and Milk. AJVS. 2014; 40(1): 24-28. doi:10.5455/ajvs.47307

Web Style

Tharwat M. Elshemey, Amir H. Abd-Elrahman. Evaluation of a Rapid Immunochromatographic Test for Detection of Brucella Abortus Antibodies in Egyptian Cattle Sera and Milk. https://www.alexjvs.com/?mno=47307 [Access: September 12, 2024]. doi:10.5455/ajvs.47307

AMA (American Medical Association) Style

Tharwat M. Elshemey, Amir H. Abd-Elrahman. Evaluation of a Rapid Immunochromatographic Test for Detection of Brucella Abortus Antibodies in Egyptian Cattle Sera and Milk. AJVS. 2014; 40(1): 24-28. doi:10.5455/ajvs.47307


Vancouver/ICMJE Style

Tharwat M. Elshemey, Amir H. Abd-Elrahman. Evaluation of a Rapid Immunochromatographic Test for Detection of Brucella Abortus Antibodies in Egyptian Cattle Sera and Milk. AJVS. (2014), [cited September 12, 2024]; 40(1): 24-28. doi:10.5455/ajvs.47307


Harvard Style

Tharwat M. Elshemey, Amir H. Abd-Elrahman (2014) Evaluation of a Rapid Immunochromatographic Test for Detection of Brucella Abortus Antibodies in Egyptian Cattle Sera and Milk. AJVS, 40 (1), 24-28. doi:10.5455/ajvs.47307


Turabian Style

Tharwat M. Elshemey, Amir H. Abd-Elrahman. 2014. Evaluation of a Rapid Immunochromatographic Test for Detection of Brucella Abortus Antibodies in Egyptian Cattle Sera and Milk. Alexandria Journal of Veterinary Sciences, 40 (1), 24-28. doi:10.5455/ajvs.47307


Chicago Style

Tharwat M. Elshemey, Amir H. Abd-Elrahman. "Evaluation of a Rapid Immunochromatographic Test for Detection of Brucella Abortus Antibodies in Egyptian Cattle Sera and Milk." Alexandria Journal of Veterinary Sciences 40 (2014), 24-28. doi:10.5455/ajvs.47307


MLA (The Modern Language Association) Style

Tharwat M. Elshemey, Amir H. Abd-Elrahman. "Evaluation of a Rapid Immunochromatographic Test for Detection of Brucella Abortus Antibodies in Egyptian Cattle Sera and Milk." Alexandria Journal of Veterinary Sciences 40.1 (2014), 24-28. Print. doi:10.5455/ajvs.47307


APA (American Psychological Association) Style

Tharwat M. Elshemey, Amir H. Abd-Elrahman (2014) Evaluation of a Rapid Immunochromatographic Test for Detection of Brucella Abortus Antibodies in Egyptian Cattle Sera and Milk. Alexandria Journal of Veterinary Sciences, 40 (1), 24-28. doi:10.5455/ajvs.47307




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