E-ISSN 2536-9520 | ISSN 1110-2047
 
2025, Vol: 84
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Original Article
Online Published: 13 Aug 2021
 


Investigations of Some Histopathological and Molecular Changes in the Brain of Rats for Evaluation of Drowning

Rehab A. Azouz, Nermeen B. El-Borai.


Abstract
Drowning is one of the common causes of death worldwide. The pathophysiology of drowning is complicated. This study is constructed to investigate the histopathological, immunohistochemical, and molecular changes in brain tissues, to identify potential biomarkers in the postmortem diagnosis of drowning. For these purposes, 15 adult rats were subjected to drowning then brain tissues were sampled at 0, 24, and 48 hours post drowning. Histopathological findings of brain sections showed brain edema and neuronal degeneration and necrosis at 24h and severe increasing in these lesions at 48h post drowning. The histopathological findings confirmed by immunohistochemical examination that revealed time-dependent positive brown staining of caspase-3 protein expressed in the cytoplasm of neurons. Moreover, the postmortem expression of Casp3, increased in a time-dependent manner. From a forensic point, molecular and immunohistochemical detection of caspase 3 in the brain of drowned animals can be considered a valuable marker to diagnose drowning and to estimate time since drowning.

Key words: Drowning; Histopathology; Apoptosis; Brain, Caspase 3, Immunohistochemistry


 
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How to Cite this Article
Pubmed Style

Azouz RA, El-Borai NB. Investigations of Some Histopathological and Molecular Changes in the Brain of Rats for Evaluation of Drowning. AJVS. 2021; 70(2): 40-45. doi:10.5455/ajvs.87063

Web Style

Azouz RA, El-Borai NB. Investigations of Some Histopathological and Molecular Changes in the Brain of Rats for Evaluation of Drowning. https://www.alexjvs.com/?mno=87063 [Access: December 01, 2024]. doi:10.5455/ajvs.87063

AMA (American Medical Association) Style

Azouz RA, El-Borai NB. Investigations of Some Histopathological and Molecular Changes in the Brain of Rats for Evaluation of Drowning. AJVS. 2021; 70(2): 40-45. doi:10.5455/ajvs.87063


Vancouver/ICMJE Style

Azouz RA, El-Borai NB. Investigations of Some Histopathological and Molecular Changes in the Brain of Rats for Evaluation of Drowning. AJVS. (2021), [cited December 01, 2024]; 70(2): 40-45. doi:10.5455/ajvs.87063


Harvard Style

Azouz, R. A. & El-Borai, . N. B. (2021) Investigations of Some Histopathological and Molecular Changes in the Brain of Rats for Evaluation of Drowning. AJVS, 70 (2), 40-45. doi:10.5455/ajvs.87063


Turabian Style

Azouz, Rehab A., and Nermeen B. El-Borai. 2021. Investigations of Some Histopathological and Molecular Changes in the Brain of Rats for Evaluation of Drowning. Alexandria Journal of Veterinary Sciences, 70 (2), 40-45. doi:10.5455/ajvs.87063


Chicago Style

Azouz, Rehab A., and Nermeen B. El-Borai. "Investigations of Some Histopathological and Molecular Changes in the Brain of Rats for Evaluation of Drowning." Alexandria Journal of Veterinary Sciences 70 (2021), 40-45. doi:10.5455/ajvs.87063


MLA (The Modern Language Association) Style

Azouz, Rehab A., and Nermeen B. El-Borai. "Investigations of Some Histopathological and Molecular Changes in the Brain of Rats for Evaluation of Drowning." Alexandria Journal of Veterinary Sciences 70.2 (2021), 40-45. Print. doi:10.5455/ajvs.87063


APA (American Psychological Association) Style

Azouz, R. A. & El-Borai, . N. B. (2021) Investigations of Some Histopathological and Molecular Changes in the Brain of Rats for Evaluation of Drowning. Alexandria Journal of Veterinary Sciences, 70 (2), 40-45. doi:10.5455/ajvs.87063




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    About Alexandria Journal of Veterinary Sciences

    The Alexandria Journal of Veterinary Sciences (AJVS) Official Journal of Faculty of Veterinary Medicine, Alexandria University   ISSN: 1110 ... Read more.



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