E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Article 


Enterotoxigenic Profiles of Bacillus Cereus Isolated from Manufacturing Steps of Traditional Soft Cheese Plant

Dina Michel, Abbas Amin, Amr Amer, Hussien S. Abo El-Makarem, Sally Ashry.

Abstract
Microbial contamination of soft cheese may originate from various sources such as contaminated equipment or from the workers themselves during cheese production. The study was aimed to characterize Bacillus cereus using culture-based and PCR techniques among 285 samples along Damietta and Kareish cheese producing line in a small cheese plant in Alexandria governorate including raw milk, cheese, whey, rennet, water, and swabs from all contacts surfaces. Overall of 10.90 % and 10 % among raw milk, cheese, whey, rennet, water, and swabs from all contacts surfaces were positive for Bacillus species in Damietta and Kareish cheese producing line. The incidence of Bacillus cereus in receiving raw milk, curd, whey, fresh cheese samples were 6.66, 13.33 and 13.33%, respectively for traditional Damietta cheese plant. Bacillus cereus is found in only 6.66 % in receiving raw milk, pasteurized milk and fresh cheese samples for traditional Kareish cheese plant. Hbl, Nhe and Cytk genes were detected in Bacillus cereus isolated from curd and Damietta cheese. Nhe gene was detected in Bacillus cereus isolated from raw milk, pasteurized milk and Kareish cheese While, Ces gene was detected only in Bacillus cereus isolated from fresh Kareish cheese samples. The prevalence of Eneterotoxigenic virulent B. cereus poses public health threats to consumers. HACCP should be implemented for all steps of manufacture in order to produce a safe and good quality dairy product.

Key words: Bacillus cereus, Damietta cheese, Kareish cheese, virulent genes,


 
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How to Cite this Article
Pubmed Style

Michel D, Amin A, Amer A, El-Makarem HSA, Ashry S. Enterotoxigenic Profiles of Bacillus Cereus Isolated from Manufacturing Steps of Traditional Soft Cheese Plant. AJVS. 2020; 66(1): 45-51. doi:10.5455/ajvs.113229


Web Style

Michel D, Amin A, Amer A, El-Makarem HSA, Ashry S. Enterotoxigenic Profiles of Bacillus Cereus Isolated from Manufacturing Steps of Traditional Soft Cheese Plant. http://www.alexjvs.com/?mno=113229 [Access: July 22, 2020]. doi:10.5455/ajvs.113229


AMA (American Medical Association) Style

Michel D, Amin A, Amer A, El-Makarem HSA, Ashry S. Enterotoxigenic Profiles of Bacillus Cereus Isolated from Manufacturing Steps of Traditional Soft Cheese Plant. AJVS. 2020; 66(1): 45-51. doi:10.5455/ajvs.113229



Vancouver/ICMJE Style

Michel D, Amin A, Amer A, El-Makarem HSA, Ashry S. Enterotoxigenic Profiles of Bacillus Cereus Isolated from Manufacturing Steps of Traditional Soft Cheese Plant. AJVS. (2020), [cited July 22, 2020]; 66(1): 45-51. doi:10.5455/ajvs.113229



Harvard Style

Michel, D., Amin, . A., Amer, . A., El-Makarem, . H. S. A. & Ashry, . S. (2020) Enterotoxigenic Profiles of Bacillus Cereus Isolated from Manufacturing Steps of Traditional Soft Cheese Plant. AJVS, 66 (1), 45-51. doi:10.5455/ajvs.113229



Turabian Style

Michel, Dina, Abbas Amin, Amr Amer, Hussien S. Abo El-Makarem, and Sally Ashry. 2020. Enterotoxigenic Profiles of Bacillus Cereus Isolated from Manufacturing Steps of Traditional Soft Cheese Plant. Alexandria Journal of Veterinary Sciences, 66 (1), 45-51. doi:10.5455/ajvs.113229



Chicago Style

Michel, Dina, Abbas Amin, Amr Amer, Hussien S. Abo El-Makarem, and Sally Ashry. "Enterotoxigenic Profiles of Bacillus Cereus Isolated from Manufacturing Steps of Traditional Soft Cheese Plant." Alexandria Journal of Veterinary Sciences 66 (2020), 45-51. doi:10.5455/ajvs.113229



MLA (The Modern Language Association) Style

Michel, Dina, Abbas Amin, Amr Amer, Hussien S. Abo El-Makarem, and Sally Ashry. "Enterotoxigenic Profiles of Bacillus Cereus Isolated from Manufacturing Steps of Traditional Soft Cheese Plant." Alexandria Journal of Veterinary Sciences 66.1 (2020), 45-51. Print. doi:10.5455/ajvs.113229



APA (American Psychological Association) Style

Michel, D., Amin, . A., Amer, . A., El-Makarem, . H. S. A. & Ashry, . S. (2020) Enterotoxigenic Profiles of Bacillus Cereus Isolated from Manufacturing Steps of Traditional Soft Cheese Plant. Alexandria Journal of Veterinary Sciences, 66 (1), 45-51. doi:10.5455/ajvs.113229





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