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Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium

Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed.

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Abstract
This study was designed to investigate the possibility of cadmium (Cd) to induce oxidative stress and biochemical perturbations in Nile tilapia liver and gills and the role of Vitamin C (Vit. C) in alleviating its toxic effects. Nile tilapia fish were randomly divided into four groups of thirteen each, group one served as control without any treatment, group two exposed to Cd (5mg/liter water), group three supplemented with vitamin C (Vit.C) (500mg/kg diet), and group four exposed to Cd plus Vit. C. The exposure to Cd caused increase in Liver aminotransferases (AST and ALT), elevation in lipid peroxidation (LPO), activity of catalase (CAT) enzyme, and the activity of glutathione S-transferase (GST). The urea and creatinine levels were not affected. However, reduction in the activity of glutathione peroxidase (GPx) was observed. An increase in reduced glutathione (GSH) content was also observed and in gills there were no significant changes in LPO, antioxidant enzymes activity and GSH level. Vit.C supplementation in Cd-induced oxidative stress of Nile tilapia maintained Liver AST and ALT near normal level and modulated LPO, CAT, GST, GPx and GSH level in liver. It is concluded that Vit.C scavenges reactive oxygen species and render a protective effect against Cd toxicity.

Key words: Nile tilapia (Oreochromis niloticus); heavy metals; Toxicity; Biomarkers; Lipid peroxidation Abbreviations


 
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REFERENCES
Abdel-Tawwab, M., Shalaby, A. M. E., Ahmad, M. H. Khattab, Y. A. E. 2004. Effect of supplemental dietary L-ascorbic acid (Vitamin C) on mercury detoxication, physiological aspects and growth performance of Nile tilapia (Oreochromus niloticus L.). Annals of Agricult. Sci., 39, (2): 883-895.
Aebi. 1984. Catalase in vitro. Methods Enzymol. 105:121-6. [DOI via Crossref]   
Ahmad, S., 1995. Oxidative stress from environmental pollutants, Archives of Insect Biochem. Physiol. 29: 861-866. [DOI via Crossref]   
Almeida, J.A., Barreto, R.E., Novelli, E.L.B., Castro, F.J., Moron, S.E. 2009. Oxidative stress biomarkers and aggressive behavior in fish exposed to aquatic cadmium contamination, Neotrop. Ichthyol 7: 103-108. [DOI via Crossref]   
Asagba, S.O., Eriyamremu, G.E., Igberaese, M.E. 2008. Bioaccumulation of cadmium and its biochemical effect on selected tissues of the catfish (Clarias gariepinus), Fish. Phsiol Biochem.34: 61-69. [DOI via Crossref]   
Atli, G, Canli, M. 2007. Enzymatic responses to metal exposures in a freshwater fish Oreochromis niloticus, Comp. Biochem. Physiol. C: 145: 282-287.
Atli, G, Canli, M. 2008. Responses of metallothionein and reduced glutathione in a freshwater fish Oreochromis niloticus following metal exposures. Environ. Toxicol. Pharmacol. 25: 33-38. [DOI via Crossref]   
Atli, G., Canli, M. 2010. Response of antioxidant system of freshwater fish Oreochromis niloticus to acute and chronic metal (Cd, Cu, Cr, Zn, Fe), Ecotoxicol. Environ. Saf. 73: 1884-1889. [DOI via Crossref]   
Bagchi, D. Bagchi, M. Hassoun, EA. Stohs, SJ. 1996. Cadmium induced excretion of urinary lipid metabolites, DNA damage, glutathione depletion, and hepatic lipid peroxidation in Sprague Dawley rats. Biol. Trace Elem. Res. 52:143-154. [DOI via Crossref]   
Bagchi, D. Joshi, SS. Bagchi, M. Balmoori, J. Benner, EJ. Kuszynski, CA. Stohs, SJ. 2000. Cadmium and chromium-induced oxidative stress, DNA damage, and apoptotic cell death in cultured human chronic myelogenous leukemic K562 cells, promyelocytic leukemic HL-60 cells, and normal human peripheral blood mononuclear cells. J. Biochem. Mol. Toxicol. 14: 33-41. [DOI via Crossref]   
Basha, S. P., Rani, U. A. 2003. Cadmium- induced antioxidant defense mechanism in freshwater teleost Oreochromis mossambicus (Tilapia). Ecotoxicol. Environ. Saf. 56, 218-221. [DOI via Crossref]   
Berntssen, M.H.G., Lundebye, A.K., Hamre, K. 2000. Tissue lipid peroxidative responses in Atlantic salmon (Salmo salar L.) parr fed high levels of dietary copper and cadmium, Fish Physiol. Biochem. 23: 35-48. [DOI via Crossref]   
Brake, I. 1997. Immune status role of vitamins. Feed Mix. 5(1): 21-24.
Chan, HM., Cherian, MG. 1992. Protective roles of metallothionein and glutathione in hepatotoxicity of cadmium. Toxicology 72:281-290. [DOI via Crossref]   
Chatterjee, I. B. 1973. Evolution and biosynthesis of ascorbic acid. Science, 182: 1271-1272. [DOI via Crossref]   
Coa G, Booth SL, Sadowski JA, Prior RL. 1998. Increases in human lasma antioxidant capacity after consumption of controlled diets high in fruit and vegetables. Am. J. Clin. Nutr. 68: 1081- 87.
Cirillo, T., Amodio C. R., Fasano , E., Lucisano , A., Tafuri, S., Ferrante, M. C., et al. 2012. Cadmium Accumulation and Antioxidant Responses in Sparus aurata Exposed to Waterborne Cadmium. Arch Environ Contam. Toxicol. 62:118-126. [DOI via Crossref]   
Congleton, JL., La Voie, WJ. 2001. Comparison of blood chemistry values of samples collected from juvenile chinook salmon by three methods. J. Aqualt. Health 13:168-172.
Cuypers, A., Plusquin, M., Remans, T., Jozefczak, M, Keunen, E., Gielen, H. et al. 2010. Cadmium stress: An oxidative challenge. Biometals 23:927-940 [DOI via Crossref]   
Cope, W. G., Wiener, J., Steingraeber, M. T., Atchison, G. J. 1994. Cadmium, metal- binding proteins, and growth in bluegill (Lepomis macrochirus) exposed to contaminated sediments from the upper Mississippi River basin. Can. J. Fish Aquat. Sci, 51: 1356-1367. [DOI via Crossref]   
Durmaz, H., Sevgiler, Y., Üner, N. 2006. Tissue-specific antioxidative and neurotoxic responses to diazinon in Oreochromis niloticus, Pestic. Biochem. Phys. 84: 215-226. [DOI via Crossref]   
Eissa, BL, Ossana, NA, Ferrari, L, Salibia´n, A. 2010. Quantitative behavioral parameters as toxicity biomarkers: Fish responses to waterborne cadmium. Arch Environ Contam Toxicol 58:1032-1039. [DOI via Crossref]   
El-Demerdash, FN., Yousef, MI., Kedwany, FS., Baghdadi, HH. 2004. Cadmium- induced changes in lipid peroxidation, blood hematology, biochemical parameters and semen quality of male rats: protective role of vitamin E and β-carotene. Food Chem. Toxicol. 42: 1563-1571. [DOI via Crossref]   
Ercal, N., Gurer-Orhan, H., Aykin-Burns, N. 2001. Toxic metals and oxidative stress Part I: mechanisms involved in metal induced oxidative damage. Curr Topics Med Chem1:529-539. [DOI via Crossref]   
El-Sokkary, GH. 2008. Melatonin and vitamin C administration ameliorate diazepam- induced oxidative stress and cell proliferation in the liver of rats. Cell Prolif 41: 168-76. [DOI via Crossref]   
Evans, P., Halliwell, B. 2001. Micronutrients: oxidant/ antioxidant status.Br J Nutr 85: 567-74. [DOI via Crossref]   
Fìrat, O., Cogun, H.Y., Aslanyavrusub, S., Kargìnb, F. 2009. Antioxidant responses and metal accumulation in tissues of Nile tilapia Oreochromis niloticus under Zn, Cd and Zn + Cd exposures, J. Appl. Toxicol. 29; 295-301. [DOI via Crossref]   
Garcia-Santos, S., Fontaý’nhas-Fernandes, A., Wilson, J.M. 2006.Cadmium tolerance in the Nile tilapia (Oreochromis niloticus) following acute exposure: Assessment of some ionoregulatory parameters, Environ. Toxicol. 21: 33-46. [DOI via Crossref]   
Geoffroy, L., Frankart,C., Eullaffroy,P., 2004. Comparisonofdifferentphysiological parameter responsesin Lemna minor and Scenedesmus obliquus exposed to herbicide flumioxazin.Environ.Pollut.131,233-241. [DOI via Crossref]   
Grosell, M, McDonald, MD, Wood, CM, Walsh, PJ. 2004. Effects of prolonged copper exposure in the marine gulf toadfish (Opsanus beta) I: Hydromineral balance and plasma nitrogenous waste products. Aquat. Toxicol. 68:249-262. [DOI via Crossref]   
Giguère, A., Campbell, P.G.C., Hare, L., Cossu-Leguille, C., 2005. Metal bioaccumulation and oxidative stress in yellow perch (Perca flavescens) collected from eight lakes along a metal contamination gradient (Cd, Cu, Zn, Ni), Can. J. Fish Aquat. Sci. 62: 563-577. [DOI via Crossref]   
Ghazaly, K.S. 1994. Efficacy of ascorbic acid (vitamin C) on experimental copper intoxication in Tilapia zillii. Bull. Nat. Inst. Oceanogr. Fish. Egypt, 20(2): 249-257.
Hansen, B.H., Romma, S., Garmo, O.A., Olsvik, P.A., Andersen, R.A. 2006. Antioxidative stress proteins and their gene expression in brown trout (Salmo trutta) from three rivers with different heavy metal levels. Comp. Biochem. Physiol. 143, 263-274.
Hellawell, J. M., 1986. Biological Indicators of Freshwater Pollution and Environmental Management. Elsevier Applied Science Publishers, London. 546 pp. [DOI via Crossref]   
Heath, A.G., 1987. Water Pollution and Fish Physiology. CRC Press, Florida, USA, p. 245.
Hollis, L., McGeer, J.C., McDonald, D.G., Wood, C.M., 1999. Cadmium accumulation, gill Cd binding, acclimation, and physiological effects during long term sublethal Cd exposure in rainbow trout. Aquat. Toxicol. 46, 101-119. [DOI via Crossref]   
Kalay, M., Ay O., Canil. M. 1999. ‘Heavy metal concentration in fish tissues from the Northeast Mediterranean Sea’, Bull. Environ. Contam. Toxicol. 63, 671-673. [DOI via Crossref]   
Karmakar, R., Banerjee, A., Datta, S., Chatterjee, M., 1999. Influence of cadmium intoxication on hepatic lipid peroxidation, glutathione level, and glutathione S- transferase and gamma-glutamyl transpeptidase activities: Correlation with chromosome aberrations in bone marrow cells, J. Environ. Pathol. Tox. 18: 277-287.
Kime, D.E. 1995. The effects of pollution on reproduction in fish. Rev. Fish., 5; 52-96. [DOI via Crossref]   
Kohen, R., Nyska, A. 2002. Oxidation of biological systems: oxidative stress phenomena, antioxidants, redox reactions, and methods for their quantification. Toxicol. Pathol. 30:620-650. [DOI via Crossref]   
Halliwell, B. and Gutteridge, J.M.C. 1999. Free Radicals in Biology and Medicine, 3rd edn., Clarendon Press, Oxford.
Kang, SA., Jang, YJ., Park, H. 1998. In vivo dual effects of vitamin C on paraquat-induced lung damage: dependence on released metals from the damaged tissue. Free Radic. Res. 28: 93-107. [DOI via Crossref]   
Habig, W. H., Pabst, M. J., Jakoby, W. B. (1974) J. Biol. Chem. 249, 7130-7139.
Li, X., C.E. Cobb, K.E. Hill, R.F. Burk and J.M. 2001. Mitochondrial uptake and recycling of ascorbic acid. Arch. Bicohem. Biophys., 387, 143-153. [DOI via Crossref]   
Gupta P, Kar A. 1998. Role of ascorbic acid in cadmium-induced thyroid dysfunction and lipid peroxidation. J Appl Toxicol 18: 317- 20. [DOI via Crossref]   
Levesque, HM., Moon, TW., Campbell, PGC., Hontela, A. 2002. Seasonal variation in carbohydrate and lipid metabolism of yellow perch (Perca flavescens) chronically exposed to metals in the field. Aquat. Toxicol. 60:257-267. [DOI via Crossref]   
Livingstone, DR. 2001. Contaminant reactive oxygen species production and oxidative damage in aquatic organisms. Mar. Pollut. Bull. 42:656-666. [DOI via Crossref]   
Livingstone, DR. 2003. Oxidative stress in aquatic organisms in relation to pollution and aquaculture. Rev. Med. Vet. 154:427- 430.
Lionetto, M.G., Caricato, R., Giordano, M.E., Pascariello, M.F., Marinosci, L., Schettion,T. 2003. Integrated use of biomarkers (acetylcholinesterase and antioxidant enzymes activities) in Mytilus galloprovincialis and Mullus barbatus in an Italian coastal marine area. Mar. Pollut. Bull. 46, 324-330. [DOI via Crossref]   
Lin Y., Tang Z.S., Cao X.W., Gan X. 2011. Acute Toxicity of Cadmium on the Antioxidant Defense Systems and Lipid Peroxidation in the Juveniles of Genetically Improved Farmed (GIFT) Tilapia Oreochromis Niloticus. J. Environ. Sci. Health., A, 5 1043-1052
Lushchak, V., Lushchak, L.P., Mota, A.A., Hermes-Lima, M. 2001. Oxidative stress and antioxidant defences in goldfish Carassius auratus during anoxia and reoxygenation. Am J Physiol. Regul. Integr. Comp. Physiol. 280:100-107.
Maracine, M., Segner, H. 1998. Cytotoxicity of metals in isolated fish cells: Importance of the cellular glutathione status. Comp. Biochem. Physiol. 120A: 83-88. [DOI via Crossref]   
May, M.J., Vernoux, T., Leaver, C., Montagu, M.V., Inzé, D. 1998. Glutathione homeostasis in plants: Implications for environmental sensing and plant development, J Exp. Bot. 49: 649-669.
Mazon, A.F., Cerqueira, C.C.C., Fernandes, M.N., 2002. Gill cellular changes induced by copper exposure in the South American tropical freshwater fish, Prochilodus scrofa. Environ. Res. 88A, 52-63. [DOI via Crossref]   
Messaoudia, I., Barhoumib, S., Saida, K., Kerken, A. 2009. Study on the sensitivity to cadmium of marine fish Salaria basilisca (Pisces: Blennidae), J Environ. Sci. 21: 1620-1624. [DOI via Crossref]   
McFarland, V.A., Inouye, L.S., Lutz, C.H.,Jarvis, A.S., Clarke, J.U., McCant, D.D., 1999. Biomarkers of oxidative stress and genotoxicity in livers of field-collected brown bullhead, Ameiurus nebulosus. Arch. Environ. Contam. Toxicol. 37, 236-241. [DOI via Crossref]   
McGeer, J.C., Szebedinszky, C., McDonald, D.G.,Wood, C.M. 2000. Effects of chronic sublethal exposure to waterborne Cu, Cd or Zn in rainbow trout. 1: iono-regulatory disturbance and metabolic costs. Aquat. Toxicol. 50, 231-243. [DOI via Crossref]   
McDonald, MD, Grosell, M. 2006. Maintaining osmotic balance with an aglomerular kidney. Comp Biochem Physiol C 143:447- 458. [DOI via Crossref]   
Monteiro, S.M., Mancera, J.M., Fontaínhas- Fernandes, A. e Sousa, M. 2005. Copper induced alterations of biochemical parameters in the gill and plasma of Oreochromis niloticus. Comp. Biochem. Physiol., 141:375-383.
Nandi, D., R.C. Patra and D. Swarup. 2005. Effect of cysteine, methionine, ascorbic acid and thiamine on arsenic- induced oxidative stress and biochemical alteration in rats. Toxicol., 211, 26-35. [DOI via Crossref]   
Mekkawy, IAA., Mahmoud, UM., Wassif, ET., Naguib, M. 2010. Effects of cadmium on some haematological and biochemical characteristics of Oreochromis niloticus (Linnaeus, 1758) dietary supplemented with tomato paste and vitamin E. Fish Physiol Biochem 37:71-84. [DOI via Crossref]   
Nriagu, J. O., H. K. T. Wong, G. Lawson & P. Daniel. 1998. Saturation of ecosystems with toxic metals in Sudbury basin, Ontario, Canada. Sci. Total Environ., 233: 99-117. ӦNER, M., ATLI, G., CANLI, M. 2008.Changes in serum biochemical parameters of freshwater fish Oreochromis Niloticus following prolonged metal (Ag, Cd, Cr, Cu, Zn) exposures. ENVIRON TOXICOL CHEM, Vol. 27, No. 2, pp. 360-366.
Ohkawa, H, Ohishi, N, Yagi, K. 1979. Assay for lipid peroxidesbin animal tissues by Thiobarbituric acid reaction. Anal Biochem 95(2):351-8. [DOI via Crossref]   
O’Neill, MD, Wesp, HM, Mensinger, AF, Hanlon, RT. 1998. Initial baseline blood chemistry of the oyster toadfish, Opsanus tau. Biol Bull 195:228-229. [DOI via Crossref]   
Osman, HAM., Ibrahim, TB., Ali, AT., Derwa, HIM. 2009. Field application of humic acid against the effect of cadmium pollution on cultured Tilapia Oreochromis niloticus. World Appl Sci J 6:1569-1575.
Pandey, S., Parvez, S., Sayeed, I., Haques, R., Bin-Hafeez, B., Raisuddin, S. 2003. Biomarkers of oxidative stress: a comparative study of river Yamuna fish Wallago attu (BI. & Schn.). Sci. Total Environ. 309, 105-115. [DOI via Crossref]   
Part, P., Lock, RA. 1983. Diffusion of calcium, cadmium and mercury in a mucous solution from rainbow trout. Comp Biochem Physiol C Toxicol Pharmacol 76:259-263. [DOI via Crossref]   
Pelgrom, SMGJ, Lamers, LPM, Lock, RAC, Balm, PHM, WendelaarBonga, SE. 1995. Interactions between copper and cadmium modify metal organ distribution in mature tilapia, Oreochromis mossambicus. Environ Pollut 90: 415-423. [DOI via Crossref]   
Pratap, H.B., Wendelaar Bonga, S.E., 1990. Effects of water-borne cadmium on plasma cortisol and glucose in the cichlid fish, Oreochromis mossambicus. Comp. Biochem. Physiol. C 95, 313-317.
Rashed, M.N. 2001. Monitoring of environmental heavy metals in fish from Nasser Lake. Environ. Int. | Volume 27, Issue 1, Pages 27-33. [DOI via Crossref]   
Shalaby, AM. 1997. Biochemical and physiological studies on metal contamination in the common carp (Cyprinus carpio L), Zagazig University (Benha branch), p 268.
Sies, H. 1999. Glutathione and its role in cellular functions. Free Radic. Biol. Med. 27: 916-921. [DOI via Crossref]   
Singh, S. and Rana, S.V.S. 2007. Amelioration of arsenic toxicity by L- Ascorbic acid in laboratory rat. J. Environ. Biol. 28(2) 377-384.
U.S. Environmental Protection Agency, 2001, pp. Update of Ambient Water Quality Criteria for Cadmium. Washington, D. C., 1- 44.
Tolbert, B. M. 1979. Ascorbic acid metabolism and physiological function. Int. J. Eit. Nutr. Res., 19: 127-142.
Varanka, Z., Rojik, I., Varanka, I., Nemcsók, J., Ábrahám, M. 2001. Biochemical and morphological changes in carp (Cyprinus carpio L.) liver following exposure to copper sulfate and tannic acid. Comp. Biochem. Physiol. C 128:467-478.
Verthac, V. and Gabaudan, J. 1994. Influence of vitamin C on the immune system of salmonids. Aquacl. Res. 25(1):21-36 [DOI via Crossref]   
Viarengo, A. 1989. Heavy metals in marine invertebrates: mechanisms of regulation and toxicity at the cellular level. CRC Critical Reviews in Aquatic Sciences, 1, 295-3 17.
Vijayavel, K., Gomathi, D., Durgabhavani, K., Balasubramanian, M.P., 2006. Sublethal effect of naphthalene on lipid peroxidation and antioxidant status in the edible marine crab Scylla serrata. Mar. Pollut. Bull. 48: 429- 433. [DOI via Crossref]   
Wedderburn, J., McFadzen, I., Scager, R.C., Bessley, A., Heath, C., Hornsby, M., Lowe,D. 2000. Biomarkers: the application of a suite of techniques to determine environmental quality. Mar. Environ. Res. 50, 431-441. [DOI via Crossref]   
Wicklund-Glynn, A., Norrgren, L., Müssener, Å. 1994. Difference in uptake of inorganic mercury and cadmium in the gills of the zebrafish, Brachydanio rerio. Aquat. Toxicol. 30, 13-26. [DOI via Crossref]   
Xu, Z., Bai, S. 2007. Effects of waterborne Cd exposure on glutathione metabolism in Nile tilapia (Oreochromis niloticus) liver, ECOTOX ENVIRON SAFE 67: 89-94. [DOI via Crossref]   
Yang, J., Chen, HC. 2003. Effects of gallium on common carp (Cyprinus carpio): Acute test, serum biochemistry, and erythrocyte morphology. Chemosphere 53:877-882. [DOI via Crossref]   
Zikic, RV., Stajn, S., Pavlovic, Z., Ognjanovic, BI., Saicic, ZS. 2001. Activities of superoxide dismutase and catalase in erytrocyte and plasma transaminases of goldfish (Carassius auratus gibelio Bloch.) exposed to cadmium. Physiol Res 50:105- 111.
You, WC., Zhang, L., Gail, MA. et al. 2000. Gastric cancer: Helicobacter pylori, serum vitamin C, and other risk factors. J. Natl. Cancer Inst. 2: 1607-12. [DOI via Crossref]   
Zaidi SMK, Banu N. 2004. Antioxidant potentials of vitamins A, E and C in modulating oxidative stress in rat brain. Clin. Chem. Acta. 340: 22-33 [DOI via Crossref]   

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Pubmed Style

EL-Gazzar AM, Ashry KE, El-Sayed YS. Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium. AJVS. 2014; 40(1): 29-43. doi:10.5455/ajvs.48333



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EL-Gazzar AM, Ashry KE, El-Sayed YS. Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium. www.scopemed.org/?mno=48333 [Access: October 24, 2017]. doi:10.5455/ajvs.48333



AMA (American Medical Association) Style

EL-Gazzar AM, Ashry KE, El-Sayed YS. Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium. AJVS. 2014; 40(1): 29-43. doi:10.5455/ajvs.48333



Vancouver/ICMJE Style

EL-Gazzar AM, Ashry KE, El-Sayed YS. Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium. AJVS. (2014), [cited October 24, 2017]; 40(1): 29-43. doi:10.5455/ajvs.48333



Harvard Style

EL-Gazzar, A. M., Ashry, K. E. & El-Sayed, Y. S. (2014) Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium. AJVS, 40 (1), 29-43. doi:10.5455/ajvs.48333



Turabian Style

EL-Gazzar, Ahmed M., Khalid E. Ashry, and Yasser S. El-Sayed. 2014. Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium. Alexandria Journal of Veterinary Sciences, 40 (1), 29-43. doi:10.5455/ajvs.48333



Chicago Style

EL-Gazzar, Ahmed M., Khalid E. Ashry, and Yasser S. El-Sayed. "Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium." Alexandria Journal of Veterinary Sciences 40 (2014), 29-43. doi:10.5455/ajvs.48333



MLA (The Modern Language Association) Style

EL-Gazzar, Ahmed M., Khalid E. Ashry, and Yasser S. El-Sayed. "Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium." Alexandria Journal of Veterinary Sciences 40.1 (2014), 29-43. Print. doi:10.5455/ajvs.48333



APA (American Psychological Association) Style

EL-Gazzar, A. M., Ashry, K. E. & El-Sayed, Y. S. (2014) Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium. Alexandria Journal of Veterinary Sciences, 40 (1), 29-43. doi:10.5455/ajvs.48333



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