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Original Research (Original Article) Open Access


Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate

Sarah E. AlBeltagi, Helmy A. Torky, Mahmoud E. Seddeek.

Abstract
Seventeen avian infectious bronchitis virus (IBV) isolates were isolated from broiler chickens showing respiratory and renal lesions. The isolated strains were characterized by real time reverse transcriptase polymerase chain reaction used for N gene, and then RT-PCR and sequence analysis of the hypervariable region 3 of the S1 spike glycoprotein gene of six isolates. Six isolates showed 87.15% to 89.71 % and 87.27% to 90.82% amino acid sequence identity and 87.61% to 89.19 % and 87.91% to 89.72% nucleotide sequence identity to the Egyptian variant 1 and the IS/885 strains, respectively. The six isolates formed a distinct phylogenetic group with the Ck/Eg/BSU-2/2011 and Ck/Eg/ BSU-3/2011 (Var 2). Amino acid and nucleotide identities between the six Egyptian isolates and variant 2 (Ck/Eg/BSU-2/2011 and Ck/Eg/BSU-3/2011) ranged from 97.27% to 100% and 97.88% to 99.38%, respectively. The results indicate that the six isolates IBV/CK/Beh/101/013/S1,IBV/CK/Beh/204/013/S1,IBV/CK/Beh/105/013/S1,IBV/CK/Beh/1011/013/S1,IBV/CK/Beh/1017/013/S1,IBV/CK/Beh/2020/013/S1 can be considered a variant 2 as Ck/Eg/BSU-2/2011 and Ck/Eg/BSU-3/2011. This study demonstrates a constant evolution of IBV in Egypt that necessitates continuous monitoring to control the spread of infections, and the development and use of vaccines based on indigenous viruses.

Key words: Antigenic variations, Infectious Bronchitis virus, RT-PCR, Sequencing.


 
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How to Cite this Article
Pubmed Style

AlBeltagi SE, Torky HA, Seddeek ME. Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate. AJVS. 2014; 40(1): 44-51. doi:10.5455/ajvs.46652


Web Style

AlBeltagi SE, Torky HA, Seddeek ME. Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate. http://www.alexjvs.com//?mno=46652 [Access: December 18, 2017]. doi:10.5455/ajvs.46652


AMA (American Medical Association) Style

AlBeltagi SE, Torky HA, Seddeek ME. Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate. AJVS. 2014; 40(1): 44-51. doi:10.5455/ajvs.46652



Vancouver/ICMJE Style

AlBeltagi SE, Torky HA, Seddeek ME. Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate. AJVS. (2014), [cited December 18, 2017]; 40(1): 44-51. doi:10.5455/ajvs.46652



Harvard Style

AlBeltagi, S. E., Torky, H. A. & Seddeek, M. E. (2014) Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate. AJVS, 40 (1), 44-51. doi:10.5455/ajvs.46652



Turabian Style

AlBeltagi, Sarah E., Helmy A. Torky, and Mahmoud E. Seddeek. 2014. Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate. Alexandria Journal of Veterinary Sciences, 40 (1), 44-51. doi:10.5455/ajvs.46652



Chicago Style

AlBeltagi, Sarah E., Helmy A. Torky, and Mahmoud E. Seddeek. "Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate." Alexandria Journal of Veterinary Sciences 40 (2014), 44-51. doi:10.5455/ajvs.46652



MLA (The Modern Language Association) Style

AlBeltagi, Sarah E., Helmy A. Torky, and Mahmoud E. Seddeek. "Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate." Alexandria Journal of Veterinary Sciences 40.1 (2014), 44-51. Print. doi:10.5455/ajvs.46652



APA (American Psychological Association) Style

AlBeltagi, S. E., Torky, H. A. & Seddeek, M. E. (2014) Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate. Alexandria Journal of Veterinary Sciences, 40 (1), 44-51. doi:10.5455/ajvs.46652





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