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Molecular Characterization of Yersinia Enterocolitica Isolated From Chicken Meat Samples

Safaa M. Shabana, Samy A. Khalil, Abd-El-Halem M. Hegazy.

Cited by (1)

Abstract
The present study was carried out for identification and molecular analysis of Yersinia enterocolitica isolated from fresh raw chicken meat samples. A total of 114 chicken meat samples were collected randomly from different chicken shops in the Kafr El- Sheikh city and tested by culturing and PCR methods. Twenty isolates out of 114 fresh raw chicken meat samples (17.5%) were found to be positive for Yersinia enterocolitica. Out of 57 chicken breast muscle samples 12 were positive (10.5% ) and in the same time only 8 positive samples detected in 57 chicken thigh muscle samples (7%) for isolation of Yersinia enterocolitica. A PCR-based assay was developed to detect the prevalence of Yersinia enterocolitica in chicken meat samples and to evaluate plasmid- and chromosome-borne virulence genes. The result of PCR examination revealed that, only 8 isolates out of 12 randomly selected isolates (66.6%) were positive as Yersinia enterocolitica by the specific gene of Yersinia enterocolitica (16S ribosomal RNA ). The results of detection of the virulence genes (ail, ystA and virF) were 75%, 50% and 37.5% respectively. These virulence genes were not detected in 2 examined isolates. The yadA gene was not detected in any of the 8 examined isolates by multiplex PCR technique. This study highlighted the importance of chicken meat as potential sources of pathogenic Yersinia enterocolitica.

Key words: Yersinia enterocolitica, chicken meat, PCR techniques, virulence genes


 
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This Article Cited By the following articles

Detection of Yersinia enterocolitica in Retail Chicken Meat, Mashhad, Iran
Journal of Pathogens 2018; 2018(): 1.

1
 
How to Cite this Article
Pubmed Style

Shabana SM, Khalil SA, Hegazy AM. Molecular Characterization of Yersinia Enterocolitica Isolated From Chicken Meat Samples. AJVS. 2015; 46(1): 124-129. doi:10.5455/ajvs.184190


Web Style

Shabana SM, Khalil SA, Hegazy AM. Molecular Characterization of Yersinia Enterocolitica Isolated From Chicken Meat Samples. http://www.alexjvs.com/?mno=184190 [Access: October 15, 2018]. doi:10.5455/ajvs.184190


AMA (American Medical Association) Style

Shabana SM, Khalil SA, Hegazy AM. Molecular Characterization of Yersinia Enterocolitica Isolated From Chicken Meat Samples. AJVS. 2015; 46(1): 124-129. doi:10.5455/ajvs.184190



Vancouver/ICMJE Style

Shabana SM, Khalil SA, Hegazy AM. Molecular Characterization of Yersinia Enterocolitica Isolated From Chicken Meat Samples. AJVS. (2015), [cited October 15, 2018]; 46(1): 124-129. doi:10.5455/ajvs.184190



Harvard Style

Shabana, S. M., Khalil, S. A. & Hegazy, A. M. (2015) Molecular Characterization of Yersinia Enterocolitica Isolated From Chicken Meat Samples. AJVS, 46 (1), 124-129. doi:10.5455/ajvs.184190



Turabian Style

Shabana, Safaa M., Samy A. Khalil, and Abd-El-Halem M. Hegazy. 2015. Molecular Characterization of Yersinia Enterocolitica Isolated From Chicken Meat Samples. Alexandria Journal of Veterinary Sciences, 46 (1), 124-129. doi:10.5455/ajvs.184190



Chicago Style

Shabana, Safaa M., Samy A. Khalil, and Abd-El-Halem M. Hegazy. "Molecular Characterization of Yersinia Enterocolitica Isolated From Chicken Meat Samples." Alexandria Journal of Veterinary Sciences 46 (2015), 124-129. doi:10.5455/ajvs.184190



MLA (The Modern Language Association) Style

Shabana, Safaa M., Samy A. Khalil, and Abd-El-Halem M. Hegazy. "Molecular Characterization of Yersinia Enterocolitica Isolated From Chicken Meat Samples." Alexandria Journal of Veterinary Sciences 46.1 (2015), 124-129. Print. doi:10.5455/ajvs.184190



APA (American Psychological Association) Style

Shabana, S. M., Khalil, S. A. & Hegazy, A. M. (2015) Molecular Characterization of Yersinia Enterocolitica Isolated From Chicken Meat Samples. Alexandria Journal of Veterinary Sciences, 46 (1), 124-129. doi:10.5455/ajvs.184190





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