E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Research (Original Article) Open Access


Molecular Identification of Beta-Lactamase-Producing Salmonella Enteritidis from Broiler Chickens

May K. Zaghloul, Emad M. Al-Ebshahy, Samy A. Khaliel, Hany F. Ellakany.

Abstract
The present study was aimed to investigate the prevalence of multidrug resistant S. enteritidis in broiler chicken flocks (n=15)suffering from diarrhea during the period of 2015 in El-Beheira governorate. A total of 210 tissue swabs (liver, intestine, spleen and gall bladder) were aseptically collected, processed and plated onto XLD agar medium. Only five (2.4%) isolates were identified as Salmonella using conventional biochemical methods and PCR assay based on invA gene. Out of 5 Salmonella isolates, 4 (80%) were identified as S. enteritidis using PCR assay based on sefA gene.Antimicrobial susceptibility testing revealed that all isolates of S. enteritidis were multi-resistant to two or more antimicrobial agents. Resistance tosulfisoxazole was common across all isolates. Furthermore, two (50%) penicillin-resistant isolates were tested positive for blaTEM gene using PCR assay. In addition, β-lactamase activity of these isolates was assessed on penicillin-sensitive strain of S. aureus (ATCC29213). Sensitivity of S. aureus to penicillin was significantly decreased in the presence of beta-lactamase-producing S.enteritidis in comparison with control group. The current study necessitates further exploration of genetic elements related to antibiotic resistance and underlines the need to track the evolution of β-lactamases in S. entritidis isolates in Egypt.

Key words: Key words:Salmonella enteritidis, β-lactamase, PCR, Egypt.


 
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Pubmed Style

Zaghloul MK, Al-Ebshahy EM, Khaliel SA, Ellakany HF. Molecular Identification of Beta-Lactamase-Producing Salmonella Enteritidis from Broiler Chickens. AJVS. 2017; 55(1): 204-209. doi:10.5455/ajvs.246419


Web Style

Zaghloul MK, Al-Ebshahy EM, Khaliel SA, Ellakany HF. Molecular Identification of Beta-Lactamase-Producing Salmonella Enteritidis from Broiler Chickens. http://www.alexjvs.com//?mno=246419 [Access: December 18, 2017]. doi:10.5455/ajvs.246419


AMA (American Medical Association) Style

Zaghloul MK, Al-Ebshahy EM, Khaliel SA, Ellakany HF. Molecular Identification of Beta-Lactamase-Producing Salmonella Enteritidis from Broiler Chickens. AJVS. 2017; 55(1): 204-209. doi:10.5455/ajvs.246419



Vancouver/ICMJE Style

Zaghloul MK, Al-Ebshahy EM, Khaliel SA, Ellakany HF. Molecular Identification of Beta-Lactamase-Producing Salmonella Enteritidis from Broiler Chickens. AJVS. (2017), [cited December 18, 2017]; 55(1): 204-209. doi:10.5455/ajvs.246419



Harvard Style

Zaghloul, M. K., Al-Ebshahy, E. M., Khaliel, S. A. & Ellakany, H. F. (2017) Molecular Identification of Beta-Lactamase-Producing Salmonella Enteritidis from Broiler Chickens. AJVS, 55 (1), 204-209. doi:10.5455/ajvs.246419



Turabian Style

Zaghloul, May K., Emad M. Al-Ebshahy, Samy A. Khaliel, and Hany F. Ellakany. 2017. Molecular Identification of Beta-Lactamase-Producing Salmonella Enteritidis from Broiler Chickens. Alexandria Journal of Veterinary Sciences, 55 (1), 204-209. doi:10.5455/ajvs.246419



Chicago Style

Zaghloul, May K., Emad M. Al-Ebshahy, Samy A. Khaliel, and Hany F. Ellakany. "Molecular Identification of Beta-Lactamase-Producing Salmonella Enteritidis from Broiler Chickens." Alexandria Journal of Veterinary Sciences 55 (2017), 204-209. doi:10.5455/ajvs.246419



MLA (The Modern Language Association) Style

Zaghloul, May K., Emad M. Al-Ebshahy, Samy A. Khaliel, and Hany F. Ellakany. "Molecular Identification of Beta-Lactamase-Producing Salmonella Enteritidis from Broiler Chickens." Alexandria Journal of Veterinary Sciences 55.1 (2017), 204-209. Print. doi:10.5455/ajvs.246419



APA (American Psychological Association) Style

Zaghloul, M. K., Al-Ebshahy, E. M., Khaliel, S. A. & Ellakany, H. F. (2017) Molecular Identification of Beta-Lactamase-Producing Salmonella Enteritidis from Broiler Chickens. Alexandria Journal of Veterinary Sciences, 55 (1), 204-209. doi:10.5455/ajvs.246419





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