Molecular Characterization of Vibrio Harveyi  in Diseased Shrimp

Helmy A. Torky; Gaber S. Abdellrazeq; Mona M. Hussein; Nourhan H. Ghanem

doi:10.5455/ajvs.206029

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Original Article
Online Published: 07 Jan 2017

AJVS. 2016; 51(2): 358-366

doi: 10.5455/ajvs.206029

Molecular Characterization of Vibrio Harveyi in Diseased Shrimp

Helmy A. Torky, Gaber S. Abdellrazeq, Mona M. Hussein, Nourhan H. Ghanem.

Abstract
The objective of this study was to characterize Vibrio harveyi phenotypically and by molecular methods. A total number of 420 shrimp post larvae samples were collected, 280 samples of diseased cultured shrimp and 140 samples of apparently healthy marine shrimp. The samples were subjected to bacteriological examination. Thirty three (11.7%) of cultured shrimp samples and 4 (2.8%) of marine shrimp samples were phenotypically positive for V. harveyi. Species - specific gene (toxRgene) and two virulence associated genes (vhh -Vibrio harveyi haemolysin, and partial hly- haemolysin gene) were investigated using conventional PCR.
This study proved that using of partial haemolysin gene in molecular characterization of V. harveyi is the fastest and most accurate method to identify all isolates of V. harveyi (highly pathogenic, moderately pathogenic and non-pathogenic strains) due to the presence of a single copy of haemolysin gene encoded in all isolates, although it is not in the same locus in the genome.

Key words: Molecular Characterization, Vibrio harveyi, Diseased Shrimp.

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Pubmed Style

Torky HA, Abdellrazeq GS, Hussein MM, Ghanem NH. Molecular Characterization of Vibrio Harveyi in Diseased Shrimp. AJVS. 2016; 51(2): 358-366. doi:10.5455/ajvs.206029

Web Style

Torky HA, Abdellrazeq GS, Hussein MM, Ghanem NH. Molecular Characterization of Vibrio Harveyi in Diseased Shrimp. https://www.alexjvs.com/?mno=255141 [Access: May 04, 2025]. doi:10.5455/ajvs.206029

AMA (American Medical Association) Style

Torky HA, Abdellrazeq GS, Hussein MM, Ghanem NH. Molecular Characterization of Vibrio Harveyi in Diseased Shrimp. AJVS. 2016; 51(2): 358-366. doi:10.5455/ajvs.206029

Vancouver/ICMJE Style

Torky HA, Abdellrazeq GS, Hussein MM, Ghanem NH. Molecular Characterization of Vibrio Harveyi in Diseased Shrimp. AJVS. (2016), [cited May 04, 2025]; 51(2): 358-366. doi:10.5455/ajvs.206029

Harvard Style

Torky, H. A., Abdellrazeq, . G. S., Hussein, . M. M. & Ghanem, . N. H. (2016) Molecular Characterization of Vibrio Harveyi in Diseased Shrimp. AJVS, 51 (2), 358-366. doi:10.5455/ajvs.206029

Turabian Style

Torky, Helmy A., Gaber S. Abdellrazeq, Mona M. Hussein, and Nourhan H. Ghanem. 2016. Molecular Characterization of Vibrio Harveyi in Diseased Shrimp. Alexandria Journal of Veterinary Sciences, 51 (2), 358-366. doi:10.5455/ajvs.206029

Chicago Style

Torky, Helmy A., Gaber S. Abdellrazeq, Mona M. Hussein, and Nourhan H. Ghanem. "Molecular Characterization of Vibrio Harveyi in Diseased Shrimp." Alexandria Journal of Veterinary Sciences 51 (2016), 358-366. doi:10.5455/ajvs.206029

MLA (The Modern Language Association) Style

APA (American Psychological Association) Style

Torky, H. A., Abdellrazeq, . G. S., Hussein, . M. M. & Ghanem, . N. H. (2016) Molecular Characterization of Vibrio Harveyi in Diseased Shrimp. Alexandria Journal of Veterinary Sciences, 51 (2), 358-366. doi:10.5455/ajvs.206029

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