Effects of Neem Leaves Aqueous Extract on Organ Histology, Haematological Parameters and Biochemical Indices in Catfish

Ubiogoro E. Oniovosa; Oluwasanmi O. Aina; Selim A. Alarape; Olusegun E. Babalola; Olanike K. Adeyemo

doi:10.5455/ajvs.256015

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Original Article

AJVS. 2017; 54(1): 17-24

doi: 10.5455/ajvs.256015

Effects of Neem Leaves Aqueous Extract on Organ Histology, Haematological Parameters and Biochemical Indices in Catfish

Ubiogoro E. Oniovosa, Oluwasanmi O. Aina, Selim A. Alarape, Olusegun E. Babalola, Olanike K. Adeyemo.

Abstract
Aqueous extract of neem leaves have been used on fish-farms to control of fish parasites and fish fry predators such as dragon-fly larvae. Generally, neem extract is considered of low toxicity towards non-target aquatic life. Therefore, the effects of Neem leaf (Azadirachta indica) aqueous extract on catfish were investigated using the histology of intestine, gill and liver, haematological and biochemical parameters as indices. Ninety (90) post-juvenile African catfish (Clarias gariepinus) weighing 42.40 ± 2.50g were used. Experimental groups in triplicate were exposed bi-weekly to 3.5% and 7% LC50 of neem leaf aqueous extract infused in commercial floating feed for four weeks while the control group was exposed to untreated feed. At the end of 4 weeks, blood samples were collected for biochemical and haematological analysis and organs (intestine, gills and liver) were harvested.
The tissues from all the groups showed no visible pathological changes. The haematological indices showed that the packed cell volume (PCV), haemoglobin (Hb) and red blood cell counts (RBC) were higher in the control than the treated groups. The mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin concentration (MCHC) and mean corpuscular volume (MCV) were not significantly affected by the treatment. The white blood cell counts (WBC) were significantly (p<0.05) lowered by the treatment and across the concentrations. The biochemical indices were also significantly affected. The use of Neem leaf aqueous extract in fish industry should be encouraged because of its efficiency in enhancing immunity against disease and suppression of the growth of specific pathogenic organism.

Key words: Neem Leaf Extract, Clarias gariepinus, Haematological Parameters, Biochemical indices, Tissues

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How to Cite this Article

Pubmed Style

Oniovosa UE, Aina OO, Alarape SA, Babalola OE, Adeyemo OK. Effects of Neem Leaves Aqueous Extract on Organ Histology, Haematological Parameters and Biochemical Indices in Catfish. AJVS. 2017; 54(1): 17-24. doi:10.5455/ajvs.256015

Web Style

Oniovosa UE, Aina OO, Alarape SA, Babalola OE, Adeyemo OK. Effects of Neem Leaves Aqueous Extract on Organ Histology, Haematological Parameters and Biochemical Indices in Catfish. https://www.alexjvs.com/?mno=256015 [Access: May 04, 2025]. doi:10.5455/ajvs.256015

AMA (American Medical Association) Style

Oniovosa UE, Aina OO, Alarape SA, Babalola OE, Adeyemo OK. Effects of Neem Leaves Aqueous Extract on Organ Histology, Haematological Parameters and Biochemical Indices in Catfish. AJVS. 2017; 54(1): 17-24. doi:10.5455/ajvs.256015

Vancouver/ICMJE Style

Oniovosa UE, Aina OO, Alarape SA, Babalola OE, Adeyemo OK. Effects of Neem Leaves Aqueous Extract on Organ Histology, Haematological Parameters and Biochemical Indices in Catfish. AJVS. (2017), [cited May 04, 2025]; 54(1): 17-24. doi:10.5455/ajvs.256015

Harvard Style

Oniovosa, U. E., Aina, . O. O., Alarape, . S. A., Babalola, . O. E. & Adeyemo, . O. K. (2017) Effects of Neem Leaves Aqueous Extract on Organ Histology, Haematological Parameters and Biochemical Indices in Catfish. AJVS, 54 (1), 17-24. doi:10.5455/ajvs.256015

Turabian Style

Oniovosa, Ubiogoro E., Oluwasanmi O. Aina, Selim A. Alarape, Olusegun E. Babalola, and Olanike K. Adeyemo. 2017. Effects of Neem Leaves Aqueous Extract on Organ Histology, Haematological Parameters and Biochemical Indices in Catfish. Alexandria Journal of Veterinary Sciences, 54 (1), 17-24. doi:10.5455/ajvs.256015

Chicago Style

Oniovosa, Ubiogoro E., Oluwasanmi O. Aina, Selim A. Alarape, Olusegun E. Babalola, and Olanike K. Adeyemo. "Effects of Neem Leaves Aqueous Extract on Organ Histology, Haematological Parameters and Biochemical Indices in Catfish." Alexandria Journal of Veterinary Sciences 54 (2017), 17-24. doi:10.5455/ajvs.256015

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