E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Research (Original Article) 


Isolation of Some Food Poisoning Microorganisms From Raw Milk With Special Reference to Verocytotoxigenic E. Coli

Samar A. Swelam, Abbas A. Amin, Amer A. Amer, Hussin S. Abo El-Makarem.

Abstract
Milk as it comes from a normal udder of a healthy animal contains a negligible number of harmful organisms but may be contaminated from variable sources. Forty random samples of raw milk were collected from supermarkets in Alexandria governorate for isolation and identification of some pathogenic microorganisms using conventional methods and advanced techniques. incidence of E. coli, Staph.aureus, listeria monocytogens and salmonella were 80, 45, 5 and 0%, respectively by using plating techniques. These results were confirmed by conventional biochemical identifications but by using vitek 2 compact system (automated biochemical identifications), the incidenceswere 37.5, 2.5, 0 and 0%, respectively.The incidence of Shiga toxin-producing E. coli isolated from the examined raw milk samples (4/10%) and could be identify virulence genes intofour different serotypes including O26:H11 (EHEC), O86 (EHEC), O119:H6 (ETEC) and O125:H21 (EHEC). By using multiplex PCR, it was found that O26:H11 had shigatoxin 1 gene, shigatoxin 2 gene and intimin gene; while O86 and O119:H6 serotypes had only shigatoxin1 gene. O125:H21 serotype had both shigatoxin 1 and shigatoxin 2 genes. It could be concluded that vitek 2 compact system is considered as sensitive and accurate method for identification of some food poisoning microorganisms in comparing with conventional biochemical identifications.

Key words: E. coli, Staph.aureus, listeria monocytogenes, raw milk, viteck 2 compact


 
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Pubmed Style

Swelam SA, Amin AA, Amer AA, El-Makarem HSA. Isolation of Some Food Poisoning Microorganisms From Raw Milk With Special Reference to Verocytotoxigenic E. Coli. AJVS. 2017; 54(2): 99-105. doi:10.5455/ajvs.267275


Web Style

Swelam SA, Amin AA, Amer AA, El-Makarem HSA. Isolation of Some Food Poisoning Microorganisms From Raw Milk With Special Reference to Verocytotoxigenic E. Coli. http://www.alexjvs.com/?mno=267275 [Access: July 21, 2018]. doi:10.5455/ajvs.267275


AMA (American Medical Association) Style

Swelam SA, Amin AA, Amer AA, El-Makarem HSA. Isolation of Some Food Poisoning Microorganisms From Raw Milk With Special Reference to Verocytotoxigenic E. Coli. AJVS. 2017; 54(2): 99-105. doi:10.5455/ajvs.267275



Vancouver/ICMJE Style

Swelam SA, Amin AA, Amer AA, El-Makarem HSA. Isolation of Some Food Poisoning Microorganisms From Raw Milk With Special Reference to Verocytotoxigenic E. Coli. AJVS. (2017), [cited July 21, 2018]; 54(2): 99-105. doi:10.5455/ajvs.267275



Harvard Style

Swelam, S. A., Amin, A. A., Amer, A. A. & El-Makarem, H. S. A. (2017) Isolation of Some Food Poisoning Microorganisms From Raw Milk With Special Reference to Verocytotoxigenic E. Coli. AJVS, 54 (2), 99-105. doi:10.5455/ajvs.267275



Turabian Style

Swelam, Samar A., Abbas A. Amin, Amer A. Amer, and Hussin S. Abo El-Makarem. 2017. Isolation of Some Food Poisoning Microorganisms From Raw Milk With Special Reference to Verocytotoxigenic E. Coli. Alexandria Journal of Veterinary Sciences, 54 (2), 99-105. doi:10.5455/ajvs.267275



Chicago Style

Swelam, Samar A., Abbas A. Amin, Amer A. Amer, and Hussin S. Abo El-Makarem. "Isolation of Some Food Poisoning Microorganisms From Raw Milk With Special Reference to Verocytotoxigenic E. Coli." Alexandria Journal of Veterinary Sciences 54 (2017), 99-105. doi:10.5455/ajvs.267275



MLA (The Modern Language Association) Style

Swelam, Samar A., Abbas A. Amin, Amer A. Amer, and Hussin S. Abo El-Makarem. "Isolation of Some Food Poisoning Microorganisms From Raw Milk With Special Reference to Verocytotoxigenic E. Coli." Alexandria Journal of Veterinary Sciences 54.2 (2017), 99-105. Print. doi:10.5455/ajvs.267275



APA (American Psychological Association) Style

Swelam, S. A., Amin, A. A., Amer, A. A. & El-Makarem, H. S. A. (2017) Isolation of Some Food Poisoning Microorganisms From Raw Milk With Special Reference to Verocytotoxigenic E. Coli. Alexandria Journal of Veterinary Sciences, 54 (2), 99-105. doi:10.5455/ajvs.267275





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