E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Research (Original Article) 


Isolation, Identification and Pathogenicity Characterization of Edwardsiella tarda Isolated From Oreochromis niloticus Fish Farms in Kafr-Elshiekh, Egypt

Eman Nagy, Amr Fadel, Fatma Abd Al-Moghny, Madiha S. Ibrahim.

Abstract
Edwardsiella septicemia is a systemic bacterial disease caused by Edwardsiella tarda that is considered one of the most important bacterial microorganisms causing severe economic losses due to morbidity and mortality among wide fish species in many countries. In this study Edwardsiella tarda (E. tarda) was isolated and identified from Oreochromis niloticus fish farms using traditional and molecular PCR techniques using E. tarda specific primers. Fifty-six isolates of E. tarda were isolated from 300 fish samples during spring, summer and autumn. While in winter it couldn’t be isolated from 100 samples. The summer season showed the highest prevalence with 28% then the spring season with 18% finally autamn10%. Experimental infection using the isolated E. tarda was performed to determine the pathogenicity in O. niloticus through mortality rate, clinical sings, post mortem lesions and histopathological changes that were observed in the liver, spleen, kidney and gills of the infected fish. The result also showed that there is no significant difference between in vivo and in vitro effect of antibiotic on E. tarda. Norfloxacin showed the highest protection in vivo with 90% survival while Ampicillin showed no protection. These results indicated that the antibiotic of choice for treatment of E. tarda was Norfloxacin.

Key words: Edwardsiella tarda, Oreochromis niloticus, antibiotics, histopathology


 
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Pubmed Style

Nagy E, Fadel A, Al-Moghny FA, Ibrahim MS. Isolation, Identification and Pathogenicity Characterization of Edwardsiella tarda Isolated From Oreochromis niloticus Fish Farms in Kafr-Elshiekh, Egypt. AJVS. 2018; 57(1): 171-179. doi:10.5455/ajvs.294237


Web Style

Nagy E, Fadel A, Al-Moghny FA, Ibrahim MS. Isolation, Identification and Pathogenicity Characterization of Edwardsiella tarda Isolated From Oreochromis niloticus Fish Farms in Kafr-Elshiekh, Egypt. http://www.alexjvs.com/?mno=294237 [Access: September 19, 2018]. doi:10.5455/ajvs.294237


AMA (American Medical Association) Style

Nagy E, Fadel A, Al-Moghny FA, Ibrahim MS. Isolation, Identification and Pathogenicity Characterization of Edwardsiella tarda Isolated From Oreochromis niloticus Fish Farms in Kafr-Elshiekh, Egypt. AJVS. 2018; 57(1): 171-179. doi:10.5455/ajvs.294237



Vancouver/ICMJE Style

Nagy E, Fadel A, Al-Moghny FA, Ibrahim MS. Isolation, Identification and Pathogenicity Characterization of Edwardsiella tarda Isolated From Oreochromis niloticus Fish Farms in Kafr-Elshiekh, Egypt. AJVS. (2018), [cited September 19, 2018]; 57(1): 171-179. doi:10.5455/ajvs.294237



Harvard Style

Nagy, E., Fadel, A., Al-Moghny, F. A. & Ibrahim, M. S. (2018) Isolation, Identification and Pathogenicity Characterization of Edwardsiella tarda Isolated From Oreochromis niloticus Fish Farms in Kafr-Elshiekh, Egypt. AJVS, 57 (1), 171-179. doi:10.5455/ajvs.294237



Turabian Style

Nagy, Eman, Amr Fadel, Fatma Abd Al-Moghny, and Madiha S. Ibrahim. 2018. Isolation, Identification and Pathogenicity Characterization of Edwardsiella tarda Isolated From Oreochromis niloticus Fish Farms in Kafr-Elshiekh, Egypt. Alexandria Journal of Veterinary Sciences, 57 (1), 171-179. doi:10.5455/ajvs.294237



Chicago Style

Nagy, Eman, Amr Fadel, Fatma Abd Al-Moghny, and Madiha S. Ibrahim. "Isolation, Identification and Pathogenicity Characterization of Edwardsiella tarda Isolated From Oreochromis niloticus Fish Farms in Kafr-Elshiekh, Egypt." Alexandria Journal of Veterinary Sciences 57 (2018), 171-179. doi:10.5455/ajvs.294237



MLA (The Modern Language Association) Style

Nagy, Eman, Amr Fadel, Fatma Abd Al-Moghny, and Madiha S. Ibrahim. "Isolation, Identification and Pathogenicity Characterization of Edwardsiella tarda Isolated From Oreochromis niloticus Fish Farms in Kafr-Elshiekh, Egypt." Alexandria Journal of Veterinary Sciences 57.1 (2018), 171-179. Print. doi:10.5455/ajvs.294237



APA (American Psychological Association) Style

Nagy, E., Fadel, A., Al-Moghny, F. A. & Ibrahim, M. S. (2018) Isolation, Identification and Pathogenicity Characterization of Edwardsiella tarda Isolated From Oreochromis niloticus Fish Farms in Kafr-Elshiekh, Egypt. Alexandria Journal of Veterinary Sciences, 57 (1), 171-179. doi:10.5455/ajvs.294237





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