E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Research (Original Article) 


Tropical theileriosis, epidemiology and Molecular diagnosis in cattle in New Valley Province, Egypt

Sotohy Ahmed, Osama Abd El-Hakim, Ahmed Maher, Yasser Elnaker.

Abstract
In this study during one year 294 blood samples, collected from cattle showing clinical signs arousing suspicion of theileriosis and apparently healthy neighboring animal (related one) for diagnosis of theileriosis using stained blood film and molecular diagnosis (Semi nested PCR). the result of blood film revealed that 91 out of 294 examined cattle (30.95%) were positive for the piroplasmic form (intra erythrocytic stage) of T. annulate infection, the highest prevalence of infection by theileriosis was (39.50 %) in Summer, and the lowest rate was (13.95 %) in Winter. and the highest prevalence of infection was 39.30 %, in age group1(1day 6months) and the lowest rate was 21.40%. in group 5 (> 4 Years). The Tbs-S/Tbs-A primer set was used for PCR amplification of Theileria sp. And the Ta-S/Tbs-A specific primer set was used in semi-nested PCR technique for detection of Theileria annulata. The semi-nested PCR accurately revealed (60%) positive samples The results indicated that Giemsa staining method, having 50% sensitivity, is not suitable for determination of Theileria annulata in carrier cattle, while semi-nested PCR technique can be used as a gold standard method for the mentioned aim and also can be used for screening of Theileria annulata carrier cattle in all regions. The phylogenic analysis of DNA of theileriosis revealed two sequences deposited in the GenBank database under accession numbers MK332380 and MK332382.

Key words: ; Cattle, theileria annulata, Seminested PCR, tropical theileriosis


 
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How to Cite this Article
Pubmed Style

Ahmed S, El-Hakim OA, Maher A, Elnaker Y. Tropical theileriosis, epidemiology and Molecular diagnosis in cattle in New Valley Province, Egypt. AJVS. 2019; 63(1): 31-41. doi:10.5455/ajvs.58560


Web Style

Ahmed S, El-Hakim OA, Maher A, Elnaker Y. Tropical theileriosis, epidemiology and Molecular diagnosis in cattle in New Valley Province, Egypt. http://www.alexjvs.com/?mno=58560 [Access: November 17, 2019]. doi:10.5455/ajvs.58560


AMA (American Medical Association) Style

Ahmed S, El-Hakim OA, Maher A, Elnaker Y. Tropical theileriosis, epidemiology and Molecular diagnosis in cattle in New Valley Province, Egypt. AJVS. 2019; 63(1): 31-41. doi:10.5455/ajvs.58560



Vancouver/ICMJE Style

Ahmed S, El-Hakim OA, Maher A, Elnaker Y. Tropical theileriosis, epidemiology and Molecular diagnosis in cattle in New Valley Province, Egypt. AJVS. (2019), [cited November 17, 2019]; 63(1): 31-41. doi:10.5455/ajvs.58560



Harvard Style

Ahmed, S., El-Hakim, . O. A., Maher, . A. & Elnaker, . Y. (2019) Tropical theileriosis, epidemiology and Molecular diagnosis in cattle in New Valley Province, Egypt. AJVS, 63 (1), 31-41. doi:10.5455/ajvs.58560



Turabian Style

Ahmed, Sotohy, Osama Abd El-Hakim, Ahmed Maher, and Yasser Elnaker. 2019. Tropical theileriosis, epidemiology and Molecular diagnosis in cattle in New Valley Province, Egypt. Alexandria Journal of Veterinary Sciences, 63 (1), 31-41. doi:10.5455/ajvs.58560



Chicago Style

Ahmed, Sotohy, Osama Abd El-Hakim, Ahmed Maher, and Yasser Elnaker. "Tropical theileriosis, epidemiology and Molecular diagnosis in cattle in New Valley Province, Egypt." Alexandria Journal of Veterinary Sciences 63 (2019), 31-41. doi:10.5455/ajvs.58560



MLA (The Modern Language Association) Style

Ahmed, Sotohy, Osama Abd El-Hakim, Ahmed Maher, and Yasser Elnaker. "Tropical theileriosis, epidemiology and Molecular diagnosis in cattle in New Valley Province, Egypt." Alexandria Journal of Veterinary Sciences 63.1 (2019), 31-41. Print. doi:10.5455/ajvs.58560



APA (American Psychological Association) Style

Ahmed, S., El-Hakim, . O. A., Maher, . A. & Elnaker, . Y. (2019) Tropical theileriosis, epidemiology and Molecular diagnosis in cattle in New Valley Province, Egypt. Alexandria Journal of Veterinary Sciences, 63 (1), 31-41. doi:10.5455/ajvs.58560





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