E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Research (Original Article) 


Immunological Assay of Ukraine Viral Hemorrhagic Vaccine in Rabbits

Disouky M. Mourad, Hanan S. El-Samahy.

Abstract
This study aimed to establish immune level and protection of Ukraine vaccine against viral rabbit hemorrhagic disease (VRHD) and determine Histopathological changes among different affected ages. 10 random vaccinated adult breeding rabbits and 10 random unvaccinated weaned rabbit (5 from mother vaccinated at mid-pregnancy &5 from mother vaccinated at labor) were selected from a total of 200 rabbits (128 females, 40 males and 32 weaned) which vaccinated with Ukraine VRHDV (viral rabbit hemorrhagic disease vaccine) as seasonal vaccination routine work at 18/12/2017, blood samples were collected for obtaining their sera to determine HI titer at 16, 23, 30, 37 and 70 days post vaccination (dpv) in adult breeding rabbits, while collected from weaned rabbits at 45, 60 old age. This rabbit farm as a whole observed for natural outbreak through one year. Adult breeding rabbits showed HI titer of 24023.851, 38077.974, 48047.703, 96095.406 and 800143.108 at 16, 23, 30, 37 and 70 dpv respectively while weaned rabbits from mother vaccinated at mid-pregnancy showed HI titer of 44061.968 & 20020.656 at 45 & 60 old age respectively also weaned rabbits from mother vaccinated at labor showed HI titer of 29067.132 & 14010.328 at 45 & 60 old age respectively. 1st Natural outbreak of RHD (rabbit hemorrhagic disease) occurred 5 months' post vaccination affecting non vaccinated weaned rabbits only, while 2nd Natural outbreak affecting both vaccinated and non-vaccinated rabbits at 9 months' post vaccination with severe mortalities. Histopathology of livers, kidneys, lungs and hearts were applied exhibiting hemorrhage, edema and inflammatory cells infiltrations in all organs with coagulative necrosis of liver and renal tubules accompanied by myocardial degeneration, these lesions varied in its severity and spreading according to affected ages. Finally, Ukraine VRHDV protect against RHD viral infection for a limited period which required to be determined in other studies.

Key words: Rabbit, viral hemorrhagic vaccine, immunity, histopathology.


 
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How to Cite this Article
Pubmed Style

Mourad DM, El-Samahy HS. Immunological Assay of Ukraine Viral Hemorrhagic Vaccine in Rabbits. AJVS. 2019; 63(1): 103-109. doi:10.5455/ajvs.62490


Web Style

Mourad DM, El-Samahy HS. Immunological Assay of Ukraine Viral Hemorrhagic Vaccine in Rabbits. http://www.alexjvs.com/?mno=62490 [Access: February 08, 2020]. doi:10.5455/ajvs.62490


AMA (American Medical Association) Style

Mourad DM, El-Samahy HS. Immunological Assay of Ukraine Viral Hemorrhagic Vaccine in Rabbits. AJVS. 2019; 63(1): 103-109. doi:10.5455/ajvs.62490



Vancouver/ICMJE Style

Mourad DM, El-Samahy HS. Immunological Assay of Ukraine Viral Hemorrhagic Vaccine in Rabbits. AJVS. (2019), [cited February 08, 2020]; 63(1): 103-109. doi:10.5455/ajvs.62490



Harvard Style

Mourad, D. M. & El-Samahy, . H. S. (2019) Immunological Assay of Ukraine Viral Hemorrhagic Vaccine in Rabbits. AJVS, 63 (1), 103-109. doi:10.5455/ajvs.62490



Turabian Style

Mourad, Disouky M., and Hanan S. El-Samahy. 2019. Immunological Assay of Ukraine Viral Hemorrhagic Vaccine in Rabbits. Alexandria Journal of Veterinary Sciences, 63 (1), 103-109. doi:10.5455/ajvs.62490



Chicago Style

Mourad, Disouky M., and Hanan S. El-Samahy. "Immunological Assay of Ukraine Viral Hemorrhagic Vaccine in Rabbits." Alexandria Journal of Veterinary Sciences 63 (2019), 103-109. doi:10.5455/ajvs.62490



MLA (The Modern Language Association) Style

Mourad, Disouky M., and Hanan S. El-Samahy. "Immunological Assay of Ukraine Viral Hemorrhagic Vaccine in Rabbits." Alexandria Journal of Veterinary Sciences 63.1 (2019), 103-109. Print. doi:10.5455/ajvs.62490



APA (American Psychological Association) Style

Mourad, D. M. & El-Samahy, . H. S. (2019) Immunological Assay of Ukraine Viral Hemorrhagic Vaccine in Rabbits. Alexandria Journal of Veterinary Sciences, 63 (1), 103-109. doi:10.5455/ajvs.62490





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