E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Research (Original Article) 


Molecular Detection and Haematological Evaluation of Trypanosomosis in Naturally Infected Sheep in Abeokuta, Nigeria

Ibironke K. Oyewusi, Michael I. Takeet, Joshua A. Oyewusi, Adewale O. Talabi, Ebenezer B. Otesile.

Abstract
An understanding of the susceptibility of different species and breeds of animals to trypanosomosis in sub-Sahara Africa would facilitate the formulation of effective control measures against the ravaging spread of African animal trypanosomosis. In this study, the prevalence of Trypanosoma species in apparently healthy Nigerian breeds of sheep was determined together with the evaluation of the Packed Cell Volume (PCV) as an indicator of the severity of infection. Blood samples of 243 sheep, including 97 West African Dwarf (WAD), 60 Yankassa, 62 Ouda, 19 Balami, and five Crossbred, were screened for trypanosomes by the Haematocrit Centrifugation Technique (HCT) and species-specific Polymerase Chain Reaction (PCR) technique. In none of the blood samples were trypanosomes detected by the HCT whereas 35.4% of the samples were positive by the PCR technique. The overall prevalence of trypanosomes by the PCR technique was T. vivax (23.05%), T. congolense Savannah (13.38%), T. congolense Forest (7.82%) and T. brucei brucei (4.53%) while the breed specific prevalence was WAD (25.8%), Yankassa (36.7%), Ouda (43.5%), Balami (47.4%) and Crossbreeds (60%). Generally, the mean PCV of infected sheep was slightly (p > 0.05) lower than that for the non-infected except for the WAD breed.
This study revealed that sub-clinical ovine trypanosomosis was endemic in the study area with Trypanosoma vivax being the most prevalent. The findings thus suggest that sheep may play a significant role as a reservoir of trypanosomes to other species of domestic animals and, as such, should be included in trypanosomosis control programmes.

Key words: Trypanosome species, Sheep, Polymerase Chain Reaction (PCR), Nigeria


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Ibironke K. Oyewusi
Articles by Michael I. Takeet
Articles by Joshua A. Oyewusi
Articles by Adewale O. Talabi
Articles by Ebenezer B. Otesile
on Google
on Google Scholar
Article Statistics
 Viewed: 184
Downloaded: 36
Cited: 0


How to Cite this Article
Pubmed Style

Oyewusi IK, Takeet MI, Oyewusi JA, Talabi AO, Otesile EB. Molecular Detection and Haematological Evaluation of Trypanosomosis in Naturally Infected Sheep in Abeokuta, Nigeria. AJVS. 2019; 63(1): 129-134. doi:10.5455/ajvs.67270


Web Style

Oyewusi IK, Takeet MI, Oyewusi JA, Talabi AO, Otesile EB. Molecular Detection and Haematological Evaluation of Trypanosomosis in Naturally Infected Sheep in Abeokuta, Nigeria. http://www.alexjvs.com/?mno=67270 [Access: November 17, 2019]. doi:10.5455/ajvs.67270


AMA (American Medical Association) Style

Oyewusi IK, Takeet MI, Oyewusi JA, Talabi AO, Otesile EB. Molecular Detection and Haematological Evaluation of Trypanosomosis in Naturally Infected Sheep in Abeokuta, Nigeria. AJVS. 2019; 63(1): 129-134. doi:10.5455/ajvs.67270



Vancouver/ICMJE Style

Oyewusi IK, Takeet MI, Oyewusi JA, Talabi AO, Otesile EB. Molecular Detection and Haematological Evaluation of Trypanosomosis in Naturally Infected Sheep in Abeokuta, Nigeria. AJVS. (2019), [cited November 17, 2019]; 63(1): 129-134. doi:10.5455/ajvs.67270



Harvard Style

Oyewusi, I. K., Takeet, . M. I., Oyewusi, . J. A., Talabi, . A. O. & Otesile, . E. B. (2019) Molecular Detection and Haematological Evaluation of Trypanosomosis in Naturally Infected Sheep in Abeokuta, Nigeria. AJVS, 63 (1), 129-134. doi:10.5455/ajvs.67270



Turabian Style

Oyewusi, Ibironke K., Michael I. Takeet, Joshua A. Oyewusi, Adewale O. Talabi, and Ebenezer B. Otesile. 2019. Molecular Detection and Haematological Evaluation of Trypanosomosis in Naturally Infected Sheep in Abeokuta, Nigeria. Alexandria Journal of Veterinary Sciences, 63 (1), 129-134. doi:10.5455/ajvs.67270



Chicago Style

Oyewusi, Ibironke K., Michael I. Takeet, Joshua A. Oyewusi, Adewale O. Talabi, and Ebenezer B. Otesile. "Molecular Detection and Haematological Evaluation of Trypanosomosis in Naturally Infected Sheep in Abeokuta, Nigeria." Alexandria Journal of Veterinary Sciences 63 (2019), 129-134. doi:10.5455/ajvs.67270



MLA (The Modern Language Association) Style

Oyewusi, Ibironke K., Michael I. Takeet, Joshua A. Oyewusi, Adewale O. Talabi, and Ebenezer B. Otesile. "Molecular Detection and Haematological Evaluation of Trypanosomosis in Naturally Infected Sheep in Abeokuta, Nigeria." Alexandria Journal of Veterinary Sciences 63.1 (2019), 129-134. Print. doi:10.5455/ajvs.67270



APA (American Psychological Association) Style

Oyewusi, I. K., Takeet, . M. I., Oyewusi, . J. A., Talabi, . A. O. & Otesile, . E. B. (2019) Molecular Detection and Haematological Evaluation of Trypanosomosis in Naturally Infected Sheep in Abeokuta, Nigeria. Alexandria Journal of Veterinary Sciences, 63 (1), 129-134. doi:10.5455/ajvs.67270





Most Viewed Articles
  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract & References

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract & References » doi: 10.5455/ajvs.45443

  • Evaluation of a Rapid Immunochromatographic Test for Detection of Brucella Abortus Antibodies in Egyptian Cattle Sera and Milk
    Tharwat M. Elshemey, Amir H. Abd-Elrahman
    AJVS. 2014; 40(1): 24-28
    » Abstract & References » doi: 10.5455/ajvs.47307

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Surgical and Histological Evaluation of the Effectiveness of Propolis on Wound Healing
    Ramadan El Sayed Abd Elwahed, Mahmoud Hussein El Kammar, Islam Reda Edrees
    AJVS. 2013; 39(1): 52-63
    » Abstract & References

  • Effect of Lead Toxicity on Mineral Metabolism and Immunological Factors in Rats
    Nabil M Taha, Mahdy S. Korshom, abd El-Wahab M Mandour, Mohamed A Lebdah, Eman S aladham
    AJVS. 2013; 39(1): 64-73
    » Abstract & References

  • Antigenic Variations of Infectious Bronchitis Virus from Broiler flocks in Al Behera Governorate
    Sarah E. AlBeltagi, Helmy A. Torky, Mahmoud E. Seddeek
    AJVS. 2014; 40(1): 44-51
    » Abstract & References » doi: 10.5455/ajvs.46652

  • Improving Production Traits for El-salam and Mandarah Chicken Strains by Crossing I- Estimation of Crossbreeding Effects for Growth Production Traits
    Ayman El-Sayed Taha, Fawzy Ali Abd El-Ghany
    AJVS. 2013; 39(1): 18-30
    » Abstract & References

  • Most Downloaded
  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract & References » doi: 10.5455/ajvs.47290

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Effect of Sex, Age and Time of the Day on Vital Parameters of Apparently Healthy West African Dwarf Goats in Abeokuta, Nigeria
    Bello, S.A., Akintunde, O. G., Sonibare, A. O., Otesile, E. B.
    AJVS. 2016; 49(2): 18-23
    » Abstract & References » doi: 10.5455/ajvs.209718

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract & References » doi: 10.5455/ajvs.45443

  • Evaluation of a Rapid Immunochromatographic Test for Detection of Brucella Abortus Antibodies in Egyptian Cattle Sera and Milk
    Tharwat M. Elshemey, Amir H. Abd-Elrahman
    AJVS. 2014; 40(1): 24-28
    » Abstract & References » doi: 10.5455/ajvs.47307

  • Detection of Some Pathogenic Organisms From Dairy Farm Milk
    Ahlam A. El-Leboudy, Amr A. Amer and Sarah Abd El Mohsen
    AJVS. 2015; 44(1): 111-118
    » Abstract & References » doi: 10.5455/ajvs.47762

  • Aerobic Bacteria Associated with Omphalitis of Chicks [English]
    Khalil, S. A. and Einas, El-Shamy
    AJVS. 2012; 37(1): 69-77
    » Abstract

  • Most Cited Articles
  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333
    Cited : 5 times [Click to see citing articles]

  • Detection of Molecular Characterization of Salmonella enterica isolated from Chicken Meat and Its Products by Using Multiplex PCR
    Heba S. Amin and Abd El-Rahman A. Abd El-Rahman
    AJVS. 2015; 46(1): 155-160
    » Abstract & References » doi: 10.5455/ajvs.196302
    Cited : 5 times [Click to see citing articles]

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232
    Cited : 5 times [Click to see citing articles]

  • Antifibrotic Effect of Curcumin on Thioacetamide Induced Liver Fibrosis
    Suzan E. Abdou, Nabil M. Taha, Abd El-Wahab A. Mandour, Mohamed A. Lebda, Hanaa R. El Hofi, Amany MSE El-Morshedy
    AJVS. 2015; 45(1): 43-50
    » Abstract & References » doi: 10.5455/ajvs.179458
    Cited : 4 times [Click to see citing articles]

  • The Growth Promoting Effect of Beta-glucan in Comparison with Sodium Butyrate in Broiler Chicks
    Abd El-Salam F. El-Sawy, Zeynab Kh. El-Maddawy, Hawary S. Ibrahiem and El-Sadeq Bo-Ghazel
    AJVS. 2015; 44(1): 23-37
    » Abstract & References » doi: 10.5455/ajvs.163992
    Cited : 4 times [Click to see citing articles]

  • Adverse Effects of Ivermectin in comparison with Rafoxanide on Male Rats
    Abd El-Salam F. El-Sawy, Zeynab Kh. El-Maddawy and Soaad A. Seed
    AJVS. 2015; 47(1): 119-128
    » Abstract & References » doi: 10.5455/ajvs.198997
    Cited : 4 times [Click to see citing articles]

  • Effectiveness of prebiotic as an alternative to the antimicrobial growth promoter on growth performance, blood constituents, intestinal healthiness and immunity of broilers.
    Mohamed S. Helal, Fatma M. Youssef, Mohamed K. Moursi, Waleed F. Khalil, Mohamed M. Abdel-Daim
    AJVS. 2015; 45(1): 13-25
    » Abstract & References » doi: 10.5455/ajvs.179869
    Cited : 4 times [Click to see citing articles]

  • Enumeration and Characterization of Aeromonas spp. Isolated from Milk and Some Dairy Products in Sharkia Governorate, Egypt
    Nagwa I. ElBalat, Salah F. Abd-ElAal, Madeha A. Ayoub, Magdy S. Elsayed
    AJVS. 2014; 40(1): 52-64
    » Abstract & References » doi: 10.5455/ajvs.49073
    Cited : 3 times [Click to see citing articles]

  • The Prevalence of Some Parasitic Zoonoses in Different Slaughtered Animal Species at Abattoir in the Mid-Delta of Egypt; with Special Reference to its Economic Implications
    Walid Elmonir, Walid Mousa, Khaled Sultan
    AJVS. 2015; 47(1): 97-103
    » Abstract & References » doi: 10.5455/ajvs.204290
    Cited : 3 times [Click to see citing articles]

  • Effect of CIDR Application Duration (7-10-14 Days) on Circulating Estrogen and Progesterone during Breeding and Non- Breeding Season in She-Camels
    Fekry M. Hussein, Kamal K. Metwelly, Mona A. Mahmoud and Mohamed H. Ragab
    AJVS. 2015; 44(1): 125-129
    » Abstract & References » doi: 10.5455/ajvs.1224
    Cited : 3 times [Click to see citing articles]