E-ISSN 2536-9520 | ISSN 1110-2047
 

Original Research (Original Article) 


Prevelence of Methicillin–Resistant Staphylococcus Aureus (MRSA)in Layer Chickens with Reference to its Ability to Form Biofilm and Enterotoxins

Aml Ahmed Bakheet, Samah Fekry Darwish.

Abstract
This descriptive study was done on 100 samples obtained from layer chicken with symptoms of arthritis and decrease egg production in Assuit Governorate Egypt. A total of 37 Staphylococcal isolates were recovered, of which 21(56.7%) identified as coagulase positive Staphylococcus aureus (SA) and 16 (43.2%) were identified as coagulase negative staphylococci (CoNS). Sensitivity test of S. aureus isolates was performed against 10 common used chemotherapeutic agents, the results showed that all tested isolates were resistant to Amoxicillin , cefoxitin, and methicillin, while all were sensitive to vancomycin and Tylosin. For phenotypic analysis of biofilm formation, Congo Red Agar (CRA) test of 21 coagulase positive S. aureus isolates revealed that 76.2 % of these isolates were positive for biofilm production with variay degrees. A total of 12 representative coagulase positive staphylococci isolates were screened by multiplex PCR targeted the 16S rRNA, nuc and mecA genes to discriminate strains and to detect methicillin resistant strains(MRSA) , another PCR assay for presence of biofilm related genes, icaA, icaD, and PCR assay to detect prevalence of enterotoxin genes (SEs) . The 12 isolates were confirmed as S. aureus .Among the 12 S. aureus strains, 11 were found carrying mecA gene and only one isolate was mecA negative, biofilm related genes were icaD gene detected in 8 of11 S.aureus strains(72.7%), while icaA gene was found in one strain only(9%)and one strain was positive for both icaA and icaD genes. All the examined 11 mecA positive S.aureus strains harbourd enterotoxins genes distributed as follow:
Sea found in (81.8%), seb (9%) and seb, sec in (9%) .Our results indicated that poultry S. aureus isolates are likely to be enterotoxigenic and biofilm producing ,and this may constitute a risk for poultry and might pose a public health hazard.

Key words: Methicillin–Resistant Staphylococcus aureus (MRSA), Enterotoxins, layer chicken, Biofilm , PCR


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Aml Ahmed Bakheet
Articles by Samah Fekry Darwish
on Google
on Google Scholar
Article Statistics
 Viewed: 645
Downloaded: 29


How to Cite this Article
Pubmed Style

Bakheet AA, Darwish SF. Prevelence of Methicillin–Resistant Staphylococcus Aureus (MRSA)in Layer Chickens with Reference to its Ability to Form Biofilm and Enterotoxins. AJVS. 2019; 63(2): 23-32. doi:10.5455/ajvs.69499


Web Style

Bakheet AA, Darwish SF. Prevelence of Methicillin–Resistant Staphylococcus Aureus (MRSA)in Layer Chickens with Reference to its Ability to Form Biofilm and Enterotoxins. http://www.alexjvs.com/?mno=69499 [Access: February 08, 2020]. doi:10.5455/ajvs.69499


AMA (American Medical Association) Style

Bakheet AA, Darwish SF. Prevelence of Methicillin–Resistant Staphylococcus Aureus (MRSA)in Layer Chickens with Reference to its Ability to Form Biofilm and Enterotoxins. AJVS. 2019; 63(2): 23-32. doi:10.5455/ajvs.69499



Vancouver/ICMJE Style

Bakheet AA, Darwish SF. Prevelence of Methicillin–Resistant Staphylococcus Aureus (MRSA)in Layer Chickens with Reference to its Ability to Form Biofilm and Enterotoxins. AJVS. (2019), [cited February 08, 2020]; 63(2): 23-32. doi:10.5455/ajvs.69499



Harvard Style

Bakheet, A. A. & Darwish, . S. F. (2019) Prevelence of Methicillin–Resistant Staphylococcus Aureus (MRSA)in Layer Chickens with Reference to its Ability to Form Biofilm and Enterotoxins. AJVS, 63 (2), 23-32. doi:10.5455/ajvs.69499



Turabian Style

Bakheet, Aml Ahmed, and Samah Fekry Darwish. 2019. Prevelence of Methicillin–Resistant Staphylococcus Aureus (MRSA)in Layer Chickens with Reference to its Ability to Form Biofilm and Enterotoxins. Alexandria Journal of Veterinary Sciences, 63 (2), 23-32. doi:10.5455/ajvs.69499



Chicago Style

Bakheet, Aml Ahmed, and Samah Fekry Darwish. "Prevelence of Methicillin–Resistant Staphylococcus Aureus (MRSA)in Layer Chickens with Reference to its Ability to Form Biofilm and Enterotoxins." Alexandria Journal of Veterinary Sciences 63 (2019), 23-32. doi:10.5455/ajvs.69499



MLA (The Modern Language Association) Style

Bakheet, Aml Ahmed, and Samah Fekry Darwish. "Prevelence of Methicillin–Resistant Staphylococcus Aureus (MRSA)in Layer Chickens with Reference to its Ability to Form Biofilm and Enterotoxins." Alexandria Journal of Veterinary Sciences 63.2 (2019), 23-32. Print. doi:10.5455/ajvs.69499



APA (American Psychological Association) Style

Bakheet, A. A. & Darwish, . S. F. (2019) Prevelence of Methicillin–Resistant Staphylococcus Aureus (MRSA)in Layer Chickens with Reference to its Ability to Form Biofilm and Enterotoxins. Alexandria Journal of Veterinary Sciences, 63 (2), 23-32. doi:10.5455/ajvs.69499





Most Viewed Articles
  • Assessment of The Efficiency of Some Chemical Disinfectants Used in poultry Farms Against Coccidiosis
    Hamed Abdel tawab Samaha, Yasser Nasr Haggag, Mohammed Al sayed Nossair, Heba Mohammed Habib
    AJVS. 2013; 39(1): 82-90
    » Abstract & References

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Molecular Characterization of Listeria Species Isolated from Frozen Fish
    Gaber S. Abdellrazeq, Ayman M. Kamar, Samya M. El-Houshy
    AJVS. 2014; 40(1): 1-15
    » Abstract & References » doi: 10.5455/ajvs.45443

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Most Downloaded
  • Comparative Study of Cellular and Humoral Immune Response to BHV-1 Vaccines in Cattle and Buffalo Calves
    Fayrouz S. Naim; Samy A. Khalil and Helmy A. Torky
    AJVS. 2016; 51(2): 367-373
    » Abstract

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232

  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333

  • Using of Polypropylene Mesh for Hernioplasty in Calves
    Mostafa M. kassam, Mahmoud H. Elkammer, Ahmed S. Korittum, Ali A. Abdel-Wahed
    AJVS. 2014; 40(1): 112-117
    » Abstract & References » doi: 10.5455/ajvs.47290

  • Influence of Combined Administration of Turmeric and Black seed on Selected Biochemical Parameters of Diabetic Rats
    Sabry M. El-Bahr, Nabil M. Taha, Mahdy A. Korshom, Abd EL-Wahab A. Mandour, Mohamed A. Lebda
    AJVS. 2014; 41(1): 19-27
    » Abstract & References » doi: 10.5455/ajvs.154650

  • Most Cited Articles
  • Physiological and Oxidative Stress Biomarkers in the Freshwater Nile Tilapia, Oreochromis niloticus L., exposed to sublethal doses of cadmium
    Ahmed M. EL-Gazzar, Khalid E. Ashry, Yasser S. El-Sayed
    AJVS. 2014; 40(1): 29-43
    » Abstract & References » doi: 10.5455/ajvs.48333
    Cited : 8 times [Click to see citing articles]

  • Growth Performance, Blood Parameters, Immune response and Carcass Traits of Broiler Chicks Fed on Graded Levels of Wheat Instead of Corn without or With Enzyme Supplementation
    Mohamed I. El-Katcha, Mosaad A. Soltan, Hany F. El-Kanwy, EL-syaed R. kawarie
    AJVS. 2014; 40(1): 95-111
    » Abstract & References » doi: 10.5455/ajvs.48232
    Cited : 7 times [Click to see citing articles]

  • Detection of Molecular Characterization of Salmonella enterica isolated from Chicken Meat and Its Products by Using Multiplex PCR
    Heba S. Amin and Abd El-Rahman A. Abd El-Rahman
    AJVS. 2015; 46(1): 155-160
    » Abstract & References » doi: 10.5455/ajvs.196302
    Cited : 5 times [Click to see citing articles]

  • Antifibrotic Effect of Curcumin on Thioacetamide Induced Liver Fibrosis
    Suzan E. Abdou, Nabil M. Taha, Abd El-Wahab A. Mandour, Mohamed A. Lebda, Hanaa R. El Hofi, Amany MSE El-Morshedy
    AJVS. 2015; 45(1): 43-50
    » Abstract & References » doi: 10.5455/ajvs.179458
    Cited : 4 times [Click to see citing articles]

  • Adverse Effects of Ivermectin in comparison with Rafoxanide on Male Rats
    Abd El-Salam F. El-Sawy, Zeynab Kh. El-Maddawy and Soaad A. Seed
    AJVS. 2015; 47(1): 119-128
    » Abstract & References » doi: 10.5455/ajvs.198997
    Cited : 4 times [Click to see citing articles]